Ptprc (NM_001109887) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD45; L-CA; Lca; RT7; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200489 representing NM_001109887 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTGTGGCTCAAACTTCTGGCCTTCAGCTTGGCCCTTCTCGGCCCAGAAGTCTTTGTCACAGGGC AAGGAACAACCGACGATGGTTTTTCATCCACGCTGATGCCTCACCTGACCCCACAGCCTGACTCACAGAC CCCCTCTGCCAGAGGAGCTGACACTCAGACACTCAGCAGCCAAGCTGACCTCACCACACTCACGGCTGCT CCCAGCGGCGAGACTGACCCACCAGGTGCCAACCTCACAACCCCTGCACCATCCACTCTGGGCTTTGCAA GCAATACCACCACAAGCACAGAAATAGCTACCCCTCAAACGAAGCCATCATGTGATGAAAAATTTGGGAA CGTTACTGTGCGTTACATCTATGATGACAGTAGTAAGAATTTTAACGCAAACCTAGAAGGTGACAAAAAA CCTAAGTGTGAATATACGGATTGTGAAAAAGAGTTAAAAAATCTACCAGAATGCTCACAGAAAAACGTCA CTCTCTCCAATGGCTCATGTACTCCAGATAAAATTATAAATTTAGATGTACCACCAGGGACTCACAACTT TAACTTAACAAACTGCACACCAGACATAGAAGCTAATACCTCAATTTGTTTGGAGTGGAAAATAAAAAAC AAATTTACCTGCGACATTCAAAAGATTTCATACAATTTCCGTTGTACACCAGAGATGAAAACATTTGCTT TGGACAAACACGGAACACTTTGGTTACACAACCTTACAGTCCGAACAAATTACACATGTGCTGCGGAAGT CCTCTACAATAACGTAATACTTTTAAAACAAGACAGAAGGGTGCAGACTGATTTTGGGACTCCAGAAATG CTTCCCCATGTTCAATGTAAGAATTCAACTAACAGCACAACATTAGTCTCCTGGGCTGAGCCAGCATCTA AACACCATGGATACATTTTATGCTATAAAAAGACCCCTTCAGAAAAATGTGAAAATTTGGCTAATGATGT GAACAGTTTTGAGGTGAAAAACCTGAGGCCTTATACAGAGTACACAGTGTCTCTATTTGCCTATGTTATT GGGAGGGTGCAACGAAATGGCCCTGCTAAGGATTGCAACTTTCGGACAAAAGCAGCTCGTCCAGGCAAAG TCAATGGTATGAAAACCTCCCGGGCGTCAGACAATAGTATAAATGTCACGTGTAATTCTCCTTATGAAAT TAATGGCCCTGAAGCGCGTTACATTTTGGAAGTCAAAAGTGGAGGTTCTTTAGTTAAAACTTTCAACCAA TCCACATGTAAATTTGTTGTAGACAATCTCTATTATTCAACTGACTATGAGTTTCTGGTCTATTTTTACA ATGGAGAGTACCTGGGAGACCCAGAAATAAAACCTCAATCAACATCTTATAATTCTAAAGCACTGATTAT ATTCCTGGTGTTTCTGATTATTGTGACATCAATAGCCCTGCTTGTTGTTTTGTATAAAATCTATGATCTG CGTAAGAAAAGATCCAGCAATTTAGATGAACAGCAGGAACTCGTTGAAAGGGATGAGGAGAAGCAGCTGA TAAATGTGGACCCAATTCATTCTGACCTTTTGTTGGAAACATACAAAAGAAAGATTGCCGATGAGGGTAG ACTGTTCCTGGCTGAATTTCAGAGCATTCCACGGGTATTCAGCAAGTTTCCCATCAAAGATGCCCGAAAG TCCCAAAACCAGAACAAAAACCGTTATGTGGACATTCTCCCCTATGATTACAACCGTGTGGAGCTCTCTG AAATAAATGGAGACGCAGGGTCCACCTACATAAATGCCAGCTACATTGATGGCTTCAAGGAACCCAGGAA ATACATTGCTGCACAAGGGCCCCGGGATGAGACAGTTGATGACTTCTGGAAGATGATCTGGGAGCAGAAG GCCACAGTTATTGTCATGGTCACACGATGTGAAGAAGGAAACAGGAACAAGTGTGCAGAATATTGGCCAT GCATGGAGGAAGGCACTCGGACTTTCAGAGATGTTGTCGTGACGATCAATGACCACAAACGATGTCCTGA TTACATTATCCAGAAGCTGAGCATTGCCCACAAAAAAGAAAAAGCAACTGGAAGAGAAGTGACTCATATT CAATTCACCAGTTGGCCAGACCATGGGGTTCCTGAAGACCCTCACCTGCTCCTGAAACTTCGACGGAGAG TTAATGCTTTTAGCAACTTCTTCAGTGGACCCATTGTGGTGCACTGCAGTGCTGGCGTTGGGCGTACAGG CACCTACATTGGAATTGATGCCATGCTCGAAAGCCTAGAAGCAGAGGGCAAAGTGGATGTCTATGGCTAT GTTGTCAACCTAAGGCGACAGAGATGTCTGATGGTGCAAGTGGAGGCCCAGTACATCCTGATCCATCAGG CCTTAGTGGAGTACAATCAATTTGGGGAAACAGAAGTGAACTTGTCTGAGTTACATTCATGTCTACAGAA TCTGAAGAAGAGAGATCCACCCAGTGACCCGTCTCCTTTGGAGGCTGAGTACCAGAGACTTCCTTCATAC AGGAGCTGGAGGACACAGCACATTGGAAATCAAGAAGAAAATAAAAAGAAAAACAGGAGTTCTAACGTTG TTCCATATGACTTTAACAGAGTGCCACTTAAGCATGAACTAGAGATGAGCAAAGAGAGCGAGGCTGAATC CGACGAATCTTCAGATGAGGACAGTGACTCGGAAGAAACCAGCAAATACATTAATGCGTCATTTGTGATG AGTTACTGGAAACCAGAAATGATGATTGCTGCCCAGGGTCCACTAAAAGAGACTATTGGTGACTTTTGGC AGATGATATTCCAAAGAAAAGTCAAGGTTATTGTGATGTTGACAGAGTTAATGAGTGGAGACCAGGAAGT CTGTGCTCAATACTGGGGAGAAGGAAAGCAGACTTATGGAGACATGGAAGTAATGTTGAAAGACACGAAC AAATCCTCAGCCTATATTCTGCGAGCATTTGAACTGAGACATTCCAAGAGAAAGGAGCCTAGAACTGTGT ACCAGTACCAGTGTACCACATGGAAAGGGGAGGAGCTCCCTGCAGAACCCAAAGATCTAGTGACTCTGAT TCAGAACATCAAACAGAAGCTTCCCAAGAGTGGCTCAGAAGGGATGAAGTACCACAAGCATGCATCAATC CTAGTCCACTGCAGGGATGGATCCCAGCAGACGGGGTTGTTCTGTGCTCTGTTCAATCTCCTGGAAAGTG CAGAAACAGAAGATGTGGTTGATGTTTTTCAAGTGGTAAAGTCTCTACGCAAAGCACGGCCGGGGATGGT GGGCAGCTTTGAGCAATACCAGTTCCTCTATGACATCATGGCCAGCATCTATCCCACCCAGAATGGACAA GTGAAGAAAGCAAACAGCCAAGACAAAATTGAATTTCATAACGAAGTGGACGGAGCCAAGCAGGACGCAA ACTGTGTTCAGCCAGCTGATCCTCTGAACAAAGCCCAGGAAGACAGCAAAGAAGTTGGAGCTTCAGAGCC TGCAAGTGGTTCTGAGGAGCCAGAACATTCTGCAAATGGTCCCATGAGCCCAGCTCTAACCCCGAGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200489 representing NM_001109887 Red=Cloning site Green=Tags(s) MYLWLKLLAFSLALLGPEVFVTGQGTTDDGFSSTLMPHLTPQPDSQTPSARGADTQTLSSQADLTTLTAA PSGETDPPGANLTTPAPSTLGFASNTTTSTEIATPQTKPSCDEKFGNVTVRYIYDDSSKNFNANLEGDKK PKCEYTDCEKELKNLPECSQKNVTLSNGSCTPDKIINLDVPPGTHNFNLTNCTPDIEANTSICLEWKIKN KFTCDIQKISYNFRCTPEMKTFALDKHGTLWLHNLTVRTNYTCAAEVLYNNVILLKQDRRVQTDFGTPEM LPHVQCKNSTNSTTLVSWAEPASKHHGYILCYKKTPSEKCENLANDVNSFEVKNLRPYTEYTVSLFAYVI GRVQRNGPAKDCNFRTKAARPGKVNGMKTSRASDNSINVTCNSPYEINGPEARYILEVKSGGSLVKTFNQ STCKFVVDNLYYSTDYEFLVYFYNGEYLGDPEIKPQSTSYNSKALIIFLVFLIIVTSIALLVVLYKIYDL RKKRSSNLDEQQELVERDEEKQLINVDPIHSDLLLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKDARK SQNQNKNRYVDILPYDYNRVELSEINGDAGSTYINASYIDGFKEPRKYIAAQGPRDETVDDFWKMIWEQK ATVIVMVTRCEEGNRNKCAEYWPCMEEGTRTFRDVVVTINDHKRCPDYIIQKLSIAHKKEKATGREVTHI QFTSWPDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLESLEAEGKVDVYGY VVNLRRQRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHSCLQNLKKRDPPSDPSPLEAEYQRLPSY RSWRTQHIGNQEENKKKNRSSNVVPYDFNRVPLKHELEMSKESEAESDESSDEDSDSEETSKYINASFVM SYWKPEMMIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELMSGDQEVCAQYWGEGKQTYGDMEVMLKDTN KSSAYILRAFELRHSKRKEPRTVYQYQCTTWKGEELPAEPKDLVTLIQNIKQKLPKSGSEGMKYHKHASI LVHCRDGSQQTGLFCALFNLLESAETEDVVDVFQVVKSLRKARPGMVGSFEQYQFLYDIMASIYPTQNGQ VKKANSQDKIEFHNEVDGAKQDANCVQPADPLNKAQEDSKEVGASEPASGSEEPEHSANGPMSPALTPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001109887
ORF Size	3570 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001109887.2
RefSeq Size	4818 bp
RefSeq ORF	3573 bp
Locus ID	24699
UniProt ID	P04157
MW	134.7 kDa
Gene Summary	member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

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