Synm (NM_001134858) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dmn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201120 representing NM_001134858 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCTGGCGGCTGCAGACCAGCTCCGAAAAGGCCGAGCTCCAGGAGCTCAATGCCCGGCTTTATG AGTACGTGTGCCGGGTGCGAGAGCTGGAGCGCGAGAACCTGCTGCTGGAGGAGGAGCTGCGCAGCCGGCT GAGCCGGGAGGACCGCTGGGCCGAAGATCAAGCGCTCTACGCCGAGGAGGCGCGCAGCTTGCGACAGCAG CTCGACGAACTGAGCTGGTCCACGGCGCTGGCCGAGGGCGAGCGCGACGCGCTGCGGAGGGAACTGCTGG ACCTGCAGCGGGAAGGTTTGGAAGCCAGCACTGCCCGCGGCCGCCTGGATGCCGAGCTAGAAGCGCAGCG GCGGGAGCTGGAGGAGGCGCTGGGCGCGCGCGCCGCCCTCGAGGCGCTGCTGGGCCGGCTGGAGGCAGAG CGACGAGACCTGGATGCGGCCCACGAGCGCCAAGTGCGAGAGCTACGCGCCCGTGCCAGCAGCCTCACCA TGCACTTCCGAGCCCGCGCCACCGGCCCCGCCGCGCCCCCGCCGCGCCTGCGGGACGTGCACGACAGCTA CGCCCTGCTAGTGGCCGAGTCGTGGAGGGAGAGCATGCAGCTGTACGAGGACGAGGTGCGCGAGCTGGAG CAGGCGCTGCGCCGCGGTCAAGAGAGCCGGCTCCAGGCGGAGGACGAGGCGCGGCTGTGCGCGCAGGAGG CGGACGCGCTGCGGAACCAGGCGCTGGAGTTGGAGCAGCTGCGTGCGAGGCTGGAGGATGAGCTGCTTCG GATGCGCGAGGAGTACGGGATGCAGGCGGAGGAGCGGCAGAGAGTGATCGACAGCCTGGAGGATGAGAAG GATGCCCTTACCTTGGCCATGGCTGACAGGCTGCGGGAGTACCAGGAGCTCCTACAGGTGAAGACGGGCC TCAGCTTGGAGGTAGCTACCTACAGGGCCTTGTTGGAAGGAGAAAGCAATCCGGAGATATTGATCTGGAC CGAGAACATTGAAAACGTGCCACGAGAGTCCAGAAACACATCCTATCGCTATACCGACTCAGTATTACAG AGGAAGAATGAGAAGAATCTCTTTCCAAGGCGAAAAACACCTTGGGCAGCTGTAAATCACAGCTCGACTT TGTATTCCAACGGGCCAGGGCACCTTGACTCACAGACAACCACAGCTGTTGGAAATGCTGCCCGAAGGGG CTTCCTGACCTCCTGCTATTCTTCCTCAGCTACAACCTCACAGCAGAAGCCCCTGGAGAAAACTGTCAGC AGTCAAGCCAGTTTAAGACCAGTCATACCAACCCATGGCTTTCTAAGAAACACTGATGCTCAAGTGAAAA CATTCTCCCATGGACCCAAAGCTGAAGGCATGGGGGACACCCTCACACGGCGGGCCACAGAGTCCGTTAT TACCAGAGAGTCATACAGAGGTCACCAGGGCAACGTGGCGGCAGGTGCTGTGAACAGCACTCAGTCAAAT GAGAGGACTGTCATTCTGGGAAAGAAATTAGAAACGCAGGCCACTAAGGAGCAAGAGAGGGACAGGTCAG GGGTGGTCAAATTAAAGCCAGAGGAGAAAATGTTTGATTCTAAAGAGAAGGCTTCGGAGGAAAGAAACTT GAGGTGGGAAGAGCTGACCAAGTTAGATGCAAGAGAGAGGGAAAGCCAGCATTTAAGGAACGAGGCCAAA GGGAAGGAAACACTGACGGGGAGAAGTAGAAAAGAAAGAGAAGTGCCCATCAGTCTAGAAGTATCTCGGG GCAGCAAATCAGAGGTGTCCACAATACACTTGCAGTCATCCGGAAGGAAGGATTCTGCCAACAGTAGGGA AAGAGAGGTAAAGACCAAAGAGACAAAGTTCCACTTGGACGGCAAGGACACTGCTGGCTCTCTGCAGAGT GACTCTACGACAGAGACCATAGCAGAAAACATCGTCACCACCATCCTTAAGCAGTTTACCCAGTCTCCAG GTGCTGAAGAGAATGTCAGTTCTTTGCCAGACACAAAGGTCACCTACCTGGACCGGAAAGAGTTTCCTGG TGACAGGAAAACCAAGACGGAAATCGTCATGGAGTCGAAGCTGACAGAAGACGTCGATGTTTCTGATGAA GCTGGCCTGGACTACCTTCTAAGCAAGGATGTCAAGGAAGTAGGACTGAAAGGGAAGTCAACAGAGACAG TGATTGGAGAGATGATCAACCTCGGTCTAAAAGGAAGGGAGGGGAGAGCCAAAGTCGTAAATGTGGAGAT TGTGGAAGAGCCCATGAGCTACGTAGGTGGTGGGAAAGTAGAGTTTTCTACCCCCTTCCAGGTGGAAGAA GTCGATGATGTGTCTCCCAGCCCCAAGGGGTTCATTGAGGAAGAGGAGGGCGAAGGAGAAACCCACATGG CATTCTCAATGCATCAACGTCAGAGGACCAACCAGCCCCAGGTGAACGTCCCTCATGTGGAAGAAGTGAC GGAGGCGGGCGACTCGGAGGGGGAGCAGAGTTATTTCGTGTCTACCCCAGATGAGTACCCTGGGGGACAT GACAGAGAAGATGATGGCTCTGTGTATGGGCAGATCCACATTGAGGAAGAGTCCACCATCCGGTACTCAT GGCAAGACGAGATTGCACAGGGGACTTGGAGAAGGAAAGCGAGAGGTGACACGGGGGAAGAGAAGCACAT GAAGGTCCTGGAGGTTCCATCGGCTTCCCTGGGTGGTGCCTTTGGATCTGCTCACGTGAAGGAAGAAGCT AGTGGTGAACTCCACGCTGAACCCACAATCATTGAGAAAGAGATCAAAATACCGCATGAATTCCACACCT CCATAAAGGGCGTCTTCTCCAGTGAGCCTCGGCATCAGTTGGTGGAGGTCATTGGGCAGCTGGAGGAAAC GTTGCCTGAGCGCATGAAGGAAGAGCTGTCTGCCCTGACCAGACAGAGTCAAGGAGAGCCAGGGAGTGTC TCAGTGGATGTAAAGAAAGTCCAGAGCTCTGCTGGCGGTTCTGTGACCTTAATGGCTGAGGTCAACCTCT CGCAGACTGTAGATGCTGATCAGTTAGACCTGGAGGAGTTGAGCAGGGACGAAGCTGGGGAAATAGAGAA GGCTGTGGAGTCAGTGGTAAGAGAGAGCTTGACCAAGCGCTCCAGCCCAGTGCCTAGAAGCCCAGACAGG GAAGATGGAGAGGAGGCGCCTGCCGGTGGCTTTCTCTTCAAGCGCTGGGCCACCAGGGAGCTGTATAGCC CGTCTGGTGAGAAGGATGATACTGACCGGGTCTTTCACAGCTCGGATCAGCGTGTTACCCAGGGCCCAGT GTCAGCCACCGTGGAAGTGACCAGCCCAACGGGTTTTGTGCAGTCTCATGTGCTGGAGGATGTAAGCCAG TCTGTAAGGCACGTTAAACTAGGCCCCACTGAGATGTGGAGGACGGAACAGGTGTCCTACGGAGGACCCA CCTCACCGGTGGTGGAGGTAAGTGAAGACTTCAGCGAGGCAGTCAGCTCCGAGGGAGCCAGCCGCTCCGT GAGGCACGTTACACTGGGTCCCCGTCAAAACCGAGTGTCCACAGAAGTCATTTTCCGAGGCTCTGTGCCT ACCGGGCAGGAGACAGGAGACACAGGAGAGCCTGACCCTGCAGCACTGTCTGTGGAAGCTGACATCTCAG GGAGTGGCAGGATGCCCGGCTCTCAGCAGGCTCACAATGAAAAGGAAACTCCTTTTCCGGGTCCTATTTC TGGGGCAGCCCAGGTTGGTAGTAACTTTTCAACTGAAGGGTCAGTGGGTACCCAGACTTTTGTTAGGCAT CTCCAGTTAGGCTCCAGAGAAGGGTTCAGAGAAGAAATCCAGTTCATAGCTCCCATCCCAGACAGGGCAG AGTGGGGAGAAGAGGATTCTGGGTACACCAGAGTGTCTCTAGGGAGGAGCACCTCCATCCAGCACATTGA CATCGTGCCTCAGAGGTACCAGGCTTCCAAGCAGGTGGCCCCACAGACATTGGGATTTAGGGACTCAGAA GGCGTAGTCCTGGTGGAGAGGTCAGCAGGTATGACCCAGGCCACTCACAATTTCACCTCAGATAGAGAAA TCCTCAAGAAGGAAGAGAACACATTCCAGAGGGTCATTTCTGGGTCCCCTCCAGACAGTGTGGGCGACAC AGGAGCAGAGGTGACAGAAAACATCAGCAGATCCTTCAGGCACATTCAAATAGGTCCTACAGAGGAGGAC CCCTCTGAATACATTGTCACCAGTGGACCCTTGTCTAAAACATTTGTTCTTGACAGTTCCGTGGCATCCC CTGGGCCGGGTGGGGGAGCAGACGGCAGTAGCACACCATGTCACATTGCACTGGGGCCCAAAGAAACTTC ATTTACCTTTCAGATGGGTGTAAGTGACACGAGAGCCATCCGCAGCTGGACCCGAGACACAGGGTCAGAA GTGGAAGCTCACAGTGTGTCTCACCACGGTGGCTGGAGAGTTGCTCACAGTAGGGATGAGCAATCAGCCA GCACCAGCTCTCAGGCCTCTGCTGGGGATGCACACCAGGCCCGTGGAGAGAAAGGCACGGAGCAGGCTGG ATTTGACAAGACGGTGCAGCTGCAAAGAATGGTAGACCAAAGGTCTGTGGCTTCAGATGAGAAGAAAGTG GCCCTTCTCTATCTAGACAATGAGGAGGAAGAGGAGGGCGAGGGATGGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201120 representing NM_001134858 Red=Cloning site Green=Tags(s) MLSWRLQTSSEKAELQELNARLYEYVCRVRELERENLLLEEELRSRLSREDRWAEDQALYAEEARSLRQQ LDELSWSTALAEGERDALRRELLDLQREGLEASTARGRLDAELEAQRRELEEALGARAALEALLGRLEAE RRDLDAAHERQVRELRARASSLTMHFRARATGPAAPPPRLRDVHDSYALLVAESWRESMQLYEDEVRELE QALRRGQESRLQAEDEARLCAQEADALRNQALELEQLRARLEDELLRMREEYGMQAEERQRVIDSLEDEK DALTLAMADRLREYQELLQVKTGLSLEVATYRALLEGESNPEILIWTENIENVPRESRNTSYRYTDSVLQ RKNEKNLFPRRKTPWAAVNHSSTLYSNGPGHLDSQTTTAVGNAARRGFLTSCYSSSATTSQQKPLEKTVS SQASLRPVIPTHGFLRNTDAQVKTFSHGPKAEGMGDTLTRRATESVITRESYRGHQGNVAAGAVNSTQSN ERTVILGKKLETQATKEQERDRSGVVKLKPEEKMFDSKEKASEERNLRWEELTKLDARERESQHLRNEAK GKETLTGRSRKEREVPISLEVSRGSKSEVSTIHLQSSGRKDSANSREREVKTKETKFHLDGKDTAGSLQS DSTTETIAENIVTTILKQFTQSPGAEENVSSLPDTKVTYLDRKEFPGDRKTKTEIVMESKLTEDVDVSDE AGLDYLLSKDVKEVGLKGKSTETVIGEMINLGLKGREGRAKVVNVEIVEEPMSYVGGGKVEFSTPFQVEE VDDVSPSPKGFIEEEEGEGETHMAFSMHQRQRTNQPQVNVPHVEEVTEAGDSEGEQSYFVSTPDEYPGGH DREDDGSVYGQIHIEEESTIRYSWQDEIAQGTWRRKARGDTGEEKHMKVLEVPSASLGGAFGSAHVKEEA SGELHAEPTIIEKEIKIPHEFHTSIKGVFSSEPRHQLVEVIGQLEETLPERMKEELSALTRQSQGEPGSV SVDVKKVQSSAGGSVTLMAEVNLSQTVDADQLDLEELSRDEAGEIEKAVESVVRESLTKRSSPVPRSPDR EDGEEAPAGGFLFKRWATRELYSPSGEKDDTDRVFHSSDQRVTQGPVSATVEVTSPTGFVQSHVLEDVSQ SVRHVKLGPTEMWRTEQVSYGGPTSPVVEVSEDFSEAVSSEGASRSVRHVTLGPRQNRVSTEVIFRGSVP TGQETGDTGEPDPAALSVEADISGSGRMPGSQQAHNEKETPFPGPISGAAQVGSNFSTEGSVGTQTFVRH LQLGSREGFREEIQFIAPIPDRAEWGEEDSGYTRVSLGRSTSIQHIDIVPQRYQASKQVAPQTLGFRDSE GVVLVERSAGMTQATHNFTSDREILKKEENTFQRVISGSPPDSVGDTGAEVTENISRSFRHIQIGPTEED PSEYIVTSGPLSKTFVLDSSVASPGPGGGADGSSTPCHIALGPKETSFTFQMGVSDTRAIRSWTRDTGSE VEAHSVSHHGGWRVAHSRDEQSASTSSQASAGDAHQARGEKGTEQAGFDKTVQLQRMVDQRSVASDEKKV ALLYLDNEEEEEGEGWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134858
ORF Size	4671 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134858.3, NP_001128330.1
RefSeq Size	7432 bp
RefSeq ORF	4674 bp
Locus ID	308709
MW	172.9 kDa
Gene Summary	human homolog is an intermediate filament protein; involved in connecting desmin the extracellular matrix [RGD, Feb 2006]

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