Slit3 (NM_031321) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Megf5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201491 representing NM_031321 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGGGCGGACCGGGGCCGGCGCCGCTGTGCGCGCCCGCCTGGCGCTGGCCTTGGCGCTGGCGA GCATCCTGAGCGGACCCCCCGCTGCTGCCTGCCCCACCAAGTGTACCTGCTCCGCCGCTAGCGTGGACTG CCACGGGCTGGGCCTGCGCGCCGTTCCCCGGGGCATCCCCCGCAACGCTGAGCGCCTTGACCTGGACAGA AATAACATCACCAGGATCACCAAAATGGACTTCACCGGCTTGAAGAATCTCCGAGTCTTGCATCTGGAAG ACAATCAGGTCAGCGTCATCGAGAGAGGCGCCTTCCAGGATCTGAAGCAGCTGGAGCGATTACGTCTGAA CAAGAACAAGCTCCAGGTCCTTCCAGAATTACTTTTCCAGAGCACACCGAAGCTCACCAGACTAGATCTG AGCGAAAACCAGATCCAGGGCATCCCGAGGAAGGCGTTCAGGGGCGTCACGGGCGTGAAGAACCTGCAAC TGGACAATAACCACATCAGCTGCATTGAAGATGGAGCCTTCCGAGCGCTGCGCGATTTGGAGATCCTCAC CCTTAACAACAACAACATCAGCCGTATCCTGGTCACCAGCTTCAACCACATGCCGAAGATCCGGACCCTG CGTCTCCACTCCAACCACCTGTACTGTGATTGTCACTTGGCCTGGCTCTCAGACTGGCTGCGACAGCGGC GGACCATTGGCCAGTTCACCCTCTGCATGGCGCCCGTGCACCTGAGAGGCTTCAGTGTAGCAGACGTGCA GAAGAAGGAGTATGTGTGTCCAGGTCCCCACTCAGAGGCTCCAGCCTGCAATGCCAACTCCCTCTCCTGC CCTTCTGCCTGCTCATGCAGTAATAACATTGTAGACTGCCGTGGGAAGGGACTGACTGAGATCCCTGCCA ACCTGCCGGAGGGCATCGTGGAAATACGCCTAGAACAGAACTCCATCAAATCCATTCCTGCAGGAGCTTT CATCCAGTACAAAAAACTGAAGCGAATAGACATCAGCAAGAATCAGATATCGGACATTGCTCCAGATGCC TTCCAGGGCCTGAAATCACTCACGTCGCTGGTGCTCTATGGGAACAAGATCACAGAGATTCCCAAAGGAC TGTTTGACGGGCTGGTGTCCCTGCAGCTGCTCCTCCTCAATGCCAACAAGATCAACTGCCTGCGGGTAAA CACCTTCCAGGACCTACAGAACCTCAATCTGCTCTCTCTGTATGACAACAAGTTGCAGACTATCAGCAAA GGGCTCTTTGCTCCGCTGCAGTCCATCCAGACCCTCCACTTAGCTCAAAACCCGTTTGTTTGCGACTGCC ACTTGAAGTGGTTGGCCGACTACCTCCAGGACAACCCCATTGAGACGAGCGGGGCCCGCTGCAGCAGCCC ACGCCGGCTGGCCAACAAGCGCATCAGCCAGATCAAAAGCAAGAAGTTCCGCTGCTCAGGCTCGGAGGAT TATCGCAACAGATTCAGCAGCGAGTGCTTCATGGACCTAGTGTGCCCCGAGAAGTGCCGTTGTGAGGGCA CCATTGTGGACTGCTCCAACCAGAAGCTCTCCCGCATCCCGAGCCACCTCCCTGAATATACCACTGACCT GCGACTGAACGACAATGACATCGCTGTGCTGGAGGCCACTGGGATCTTCAAGAAGTTGCCCAACCTGAGG AAAATAAACTTGAGCAATAATAGGATCAAGGAGGTGCGGGAGGGTGCGTTTGATGGAGCGGCAGGCGTGC AGGAACTGATGCTGACGGGGAACCAGCTGGAGACCATGCACGGACGCATGTTCCGGGGCCTCAGCGGCCT GAAGACACTGATGTTAAGGAGCAACCTGATCAGCTGCGTAAACAATGACACCTTCGCTGGCCTAAGCTCT GTGAGACTGCTGTCCCTCTATGACAATCGGATCACCACCATCAGCCCTGGAGCCTTCACCACGCTCGTCT CCCTGTCCACCATAAACCTCTTGTCTAACCCTTTCAACTGCAACTGTCACATGGCCTGGCTCGGCCGGTG GCTGAGGAAACGCCGCATCGTAAGCGGGAACCCCAGATGTCAGAAGCCCTTTTTCCTCAAGGAGATTCCT ATCCAAGATGTGGCCATCCAGGACTTCACCTGTGAAGGCAATGAAGAGAACAGCTGTCAGCTGAGTCCAC GCTGCCCCGAGCAGTGTACCTGTGTGGAGACAGTGGTGCGATGCAGCAACAGGGGTCTCCACACCCTCCC CAAGGGCATGCCAAAGGACGTGACTGAACTGTACCTGGAAGGAAATCATTTAACGGCGGTGCCCAAAGAA TTGTCCACCTTCCGACAGCTGACACTAATTGACCTGAGCAACAACAGCATCAGTATGCTGACCAATCACA CCTTCAGCAACATGTCCCACCTCTCCACACTGATCCTGAGCTACAACCGGCTGAGATGCATCCCGGTCCA TGCCTTCAACGGGCTAAGGTCACTCCGAGTGCTAACCCTCCATGGCAATGACATTTCCAGTGTTCCTGAA GGCTCCTTCAATGATCTGACGTCCCTCTCCCACCTGGCCCTGGGAATCAACCCTCTCCACTGTGACTGCA GTCTGCGTTGGTTATCAGAGTGGATAAAGGCTGGGTACAAGGAGCCTGGCATTGCCAGATGCAGTAGCCC CGAGTCCATGGCAGACAGACTCCTGCTCACCACCCCCACCCACAGGTTCCAGTGCAAAGGGCCAGTGGAC ATTAACATCGTGGCCAAGTGCAACGCCTGCCTCTCTAGCCCATGCAAGAACAACGGCACTTGCAGCCAGG ATCCGGTGGAGCAGTACCGCTGTACCTGCCCTTACAGCTACAAGGGCAAGGACTGCACCGTGCCCATCAA CACCTGCGTCCAGAACCCCTGTCAGCACGGAGGCACCTGTCACCTCAGTGAGAGCCACAGAGATGGGTTC AGCTGCTCCTGCCCCCTGGGCTTTGAGGGACAGCGGTGTGAGATCAACCCAGATGACTGTGAGGATAACG ACTGTGAAAACAGCGCCACCTGTGTGGACGGGATCAACAACTACGCATGCGTGTGCCCGCCGAACTACAC AGGGGAGCTGTGTGATGAGGTGATTGACTACTGCGTGCCCGAGATGAACCTCTGTCAGCACGAGGCCAAG TGCATCTCCCTGGACAAAGGATTCAGGTGCGAATGTGTCCCTGGCTACAGTGGAAAGCTGTGCGAGACAG ACAACGATGACTGTGTGGCTCACAAGTGTCGCCACGGAGCCCAGTGTGTGGACGCGGTCAATGGCTACAC GTGCATCTGCCCCCAGGGCTTCAGCGGGCTCTTCTGTGAGCACCCCCCACCCATGGTTCTGCTACAGACC AGCCCCTGTGACCAGTACGAGTGCCAGAATGGGGCGCAGTGCATCGTGGTACAGCAGGAGCCCACTTGCC GCTGTCCCCCAGGCTTCGCTGGGCCCAGGTGTGAGAAGCTCATCACTGTGAATTTCGTGGGCAAGGACTC CTATGTGGAACTGGCCTCTGCCAAGGTCCGGCCCCAGGCCAACATCTCCCTGCAGGTGGCCACTGACAAG GACAACGGCATCCTTCTTTACAAGGGAGACAACGACCCCCTGGCACTGGAGCTGTACCAGGGTCACGTGA GGCTGGTGTATGACAGCCTGAGCTCCCCTCCGACCACGGTGTACAGTGTGGAGACGGTGAATGATGGCCA GTTTCACAGTGTGGAGCTGGTGATGCTAAACCAGACCCTGAACCTGGTGGTAGACAAAGGAGCCCCCAAG AGCCTGGGGAAGCTCCAGAAGCAGCCAGCAGTGGGCATCAACAGTCCCCTCTATCTTGGAGGCATCCCCA CGTCTACAGGCCTCTCAGCCTTACGCCAGGGTGCAGACAGGCCGCTGGGCGGCTTCCACGGCTGTATACA CGAAGTGCGCATCAACAACGAGTTGCAGGATTTCAAAGCCCTCCCACCCCAGTCCCTGGGGGTCTCTCCC GGCTGCAAATCCTGCACTGTGTGTCGTCACGGCCTGTGTCGTTCCGTGGAGAAGGACAGCGTAGTGTGTG AGTGCCATCCGGGATGGACTGGTCCACTGTGTGATCAGGAAGCCCAGGACCCCTGCCTTGGTCACAGCTG CAGCCATGGGACATGCGTGGCGACTGGCAACTCATATGTGTGCAAGTGTGCCGAGGGCTATGAGGGACCT TTGTGTGACCAGAAGAATGACTCTGCCAATGCCTGCTCAGCCTTCAAGTGCCACCACGGGCAGTGCCACA TCTCAGATCGAGGGGAGCCCTATTGCCTGTGCCAGCCTGGCTTCAGTGGCAATCACTGTGAGCAAGAGAA TCCATGTTTGGGAGAGATAGTCCGTGAAGCCATCCGCCGCCAGAAAGATTATGCCTCCTGTGCCACAGCA TCCAAGGTGCCCATCATGGTATGCCGCGGGGGCTGCGGGAGCCAATGCTGCCAGCCGATTCGGAGCAAGC GGCGGAAATACGTCTTCCAGTGCACTGACGGCTCCTCGTTCGTGGAAGAGGTGGAGAGACACTTGGAATG TGGCTGTCGTGAGTGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201491 representing NM_031321 Red=Cloning site Green=Tags(s) MAPGRTGAGAAVRARLALALALASILSGPPAAACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDR NNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDL SENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTL RLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSC PSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDA FQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISK GLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSED YRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLR KINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSS VRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIP IQDVAIQDFTCEGNEENSCQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVPKE LSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPE GSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGPVD INIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGF SCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAK CISLDKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPPPMVLLQT SPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDK DNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPK SLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGADRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSP GCKSCTVCRHGLCRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSHGTCVATGNSYVCKCAEGYEGP LCDQKNDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQENPCLGEIVREAIRRQKDYASCATA SKVPIMVCRGGCGSQCCQPIRSKRRKYVFQCTDGSSFVEEVERHLECGCRECS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031321
ORF Size	4569 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031321.1, NP_112611.1
RefSeq Size	5210 bp
RefSeq ORF	4572 bp
Locus ID	83467
UniProt ID	O88280
MW	167.8 kDa
Gene Summary	localized to mitochondria; has less conservation with Drosophila slit than family members slit1 and slit2; may play a role in neurogenesis [RGD, Feb 2006]

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