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Hsd17b1 (NM_012851) Rat Tagged ORF Clone
CAT＃: RR201524
- TrueORF®
Hsd17b1 (Myc-DDK-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10 ug)
"NM_012851" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 17BHD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201524 representing NM_012851
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCACGGTAGTGCTCATTACCGGTTGCTCTTCTGGAATTGGCCTGCACTTGGCTGTTCGTCTGG CTTCCGACCGTTCCCAGAGCTTCAAAGTTTATGCCACATTGCGGGATCTGAAGTCACAGGGACCATTGTT GGAGGCAGCCAGGGCTCAAGGATGCCCTCCTGGCTCCCTGGAGATACTGGAATTGGATGTCAGAGACTCG GAATCTGTGGCTGCTGCCCGGGCATGCGTGACGGAGGGTCGAGTGGATGTACTGGTCTGTAATGCTGGAC GCGGCTTGTTCGGGCCGCTGGAAGCGCATGAGTTGAACGCTGTGGGTGCTGTACTGGATGTGAATGTTCT TGGGACCATCCGGATGCTTCAGGCCTTTCTGCCAGACATGAAAAGGCGCCACTCCGGGCGTGTGCTGGTG ACTGCGAGTGTGGGAGGTTTGATGGGGCTGCCATTCCACGAAGTGTACTGTGCCAGCAAGTTTGCGCTCG AAGGTCTGTGCGAGAGTCTGGCGATCCTGCTGCCGCTCTTTGGAGTCCACGTGAGCCTCATCGAGTGCGG GGCAGTGCACACAGCCTTCCATGAAAAGCTGGAGGGCGGCCCGGGCGGGGCTCTGGAGCGAGCAGATGCC CAGACCCGCCACCTCTTTGCCCACTATCAGCGTGGTTATGAGCAAGCCCTGAGCGAGGCGCAGGACCCAG AGGAGGTGACGGAGCTCTTCTTGACTGCGATGCGAGCCCCGCAGCCTGCGCTGCGCTACTTCTCCACAAA CCGCTTCCTGCCGCTGGCCCGAATGCGCACAGAGGACCCCAGTGGCAGCAGCTACGTCGAAGCCATGCAC CGCGAGGCCTTCTCTGATCTGCAGGTCCAGGAGGGTGCCAAGGCTGGGGCCCAAGTTTCAGGGGATCCAG ACACGCCCCCCAGAGCCCTCATTTGTCTCCCCGAGTGTGCAATCCCTAGGGTGACAGCTGAATTGGGATG GTCTGCATCAGATAAACCGGGTCAGAACAAATCATGTTACCAGCAAAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201524 representing NM_012851
Red=Cloning site Green=Tags(s) MDSTVVLITGCSSGIGLHLAVRLASDRSQSFKVYATLRDLKSQGPLLEAARAQGCPPGSLEILELDVRDS ESVAAARACVTEGRVDVLVCNAGRGLFGPLEAHELNAVGAVLDVNVLGTIRMLQAFLPDMKRRHSGRVLV TASVGGLMGLPFHEVYCASKFALEGLCESLAILLPLFGVHVSLIECGAVHTAFHEKLEGGPGGALERADA QTRHLFAHYQRGYEQALSEAQDPEEVTELFLTAMRAPQPALRYFSTNRFLPLARMRTEDPSGSSYVEAMH REAFSDLQVQEGAKAGAQVSGDPDTPPRALICLPECAIPRVTAELGWSASDKPGQNKSCYQQKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012851 |
| ORF Size | 1032 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012851.2, NP_036983.1 |
| RefSeq Size | 1431 bp |
| RefSeq ORF | 1035 bp |
| Locus ID | 25322 |
| UniProt ID | P51657 |
| MW | 37 kDa |
| Gene Summary | catalyzes the conversion between 17 beta-hydroxy- and 17-ketosteroids; plays a role in estradiol biosynthesis [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201524 | Hsd17b1 (untagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10 ug) |
CNY 5,856.00 |
|
| RR201524L3 | Lenti ORF clone of Hsd17b1 (Myc-DDK-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10 ug) |
CNY 6,080.00 |
|
| RR201524L4 | Lenti ORF clone of Hsd17b1 (mGFP-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10 ug) |
CNY 6,650.00 |
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