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Asap1 (NM_001044245) Rat Tagged ORF Clone
CAT＃: RR201663
- TrueORF®
Asap1 (Myc-DDK-tagged ORF) - Rat ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (Asap1), (10 ug)
"NM_001044245" in other vectors (3)
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CNY 18,430.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ddef1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201663 representing NM_001044245
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGATCTTCAGCCTCCCGGCTCTCCAGTTTTTCCTCAAGAGATTCACTATGGAATCGGATGCCGGACC AGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC CTCCAGAAAGTGAAGAAATCTGTGAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAGGAAA ACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTGGGCACTGC TTTTGTCAAGTTTTCTACACTTACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCAGGGTCTGAGC CACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACCTGAAGGGGGTCAAAGGAGATCTCAAGA AGCCATTTGACAAAGCCTGGAAAGATTATGAGATGAAGTTTACAAAAATCGAGAAGGAGAAAAGAGAGCA CGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGAGCTGAGATTGCAGAAGAAATGGAAAAGGAA CGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTTAATGAAATCAAGACCAAAAAGGGTG TGGACCTGCTGCAGAACCTCATAAAGTATTATCATGCACAATGCAATTTCTTTCAGGATGGCTTGAAAAC AGCTGATAAACTGAAACAGTACATTGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGAT GAAGAAAAGAAACAGTTAACTGCACTCCGAGACCTAATAAAATCCTCTCTGCAGCTTGATCCGAAAGAAG TAGGTGGTTTATATGTTCCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGCTACAGTATGCA TCAGCTCCAGGGCAACAAGGAGTATGGCAGTGAAAAGAAGGGCTTTCTGCTGAAGAAGAGTGACGGGATC CGGAAAGTGTGGCAAAGAAGGAAGTGTGCTGTCAAGAATGGGATACTGACCATTTCACATGCAACTTCCA ACAGGCAGCCTGCTAAGTTAAACCTCCTCACCTGCCAGGTGAAACCGAATGCTGAGGACAAGAAGTCTTT TGACCTGATATCACATAATCGAACATATCACTTTCAGGCAGAAGACGAGCAGGACTATGTAACGTGGATA TCAGTACTGACGAATAGCAAAGAAGAGGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCACAGGGGAGA ATAGCCTGGAGGATCTGACCAAAGCCATCATTGAGGATGTACAGCGACTCCCTGGGAATGACATCTGCTG TGACTGTGGCTCATCAGAACCCACATGGCTTTCCACCAACTTGGGTATTTTGACCTGTATAGAATGTTCC GGAATCCATAGGGAAATGGGGGTTCATATTTCTCGCATTCAGTCTTTGGAACTAGACAAATTAGGAACTT CTGAACTCTTGCTGGCCAAGAATGTAGGAAACAATAGTTTTAATGATATTATGGAAGCAAATTTACCCAG CCCTTCACCAAAGCCCACCCCTTCAAGTGATATGACTGTACGGAAAGAGTACATCACTGCAAAGTATGTT GACCACCGGTTCTCCAGGAAGACCTGTGCCTCTTCCTCAGCAAAGCTGAATGAATTGCTTGAGGCCATCA AGTCCAGGGACTTACTTGCACTAATCCAAGTCTATGCAGAGGGAGTGGAGTTAATGGAGCCACTGCTAGA ACCTGGGCAGGAGCTTGGGGAGACAGCTCTTCACCTTGCTGTCAGGACCGCAGACCAGACGTCTCTCCAT CTGGTGGATTTCCTTGTCCAAAACTGTGGGAACCTGGATAAGCAGACGTCCGTGGGGAATACGGTGTTGC ACTATTGCAGCATGTACGGCAAGCCCGAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCACCGTGGACAT TGTTAACCAAAATGGAGAAACTGCCTTGGATATAGCAAAGAGACTCAAAGCTACGCAGTGCGAAGATCTG CTTTCCCAAGCTAAGTCTGGGAAGTTCAACCCGCATGTCCATGTAGAGTACGAGTGGAATCTTCGACAGG ACGAGATGGATGAGAGCGATGACGACCTGGATGACAAACCAAGCCCCATCAAAAAGGAGCGCTCACCCAG ACCACAGAGCTTCTGCCACTCATCCAGCATCTCACCACAGGACAAGCTGGCACTGCCAGGGTTCAGCACT CCACGGGACAAACAGCGGCTCTCCTACGGAGCCTTCACCAACCAGATCTTCGTCTCTACGAGCACAGATT TGCCCACATCACCCACCAGTGAGGCTCCCCCTTTACCGCCTAGGAATGCCGGGAAAGGTCCAACTGGCCC ACCTTCAACACTCCCTCTAGGCACCCAGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAGAGGTCTCCT CCCCCACCACCAGGACACAAGAGAACCCTGTCTGACCCTCCCAGCCCACTACCTCATGGGCCCCCAAACA AAGGCGCAATTCCTTGGGGTAACGATGTGGGCCCATCATCTTCAAGTAAAACAGCAAACAAGTTTGAGGG ACTATCTCAGCAAGCAAGCACCAGTTCTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCTAAACTACCT CAGAAAGTGGCACTAAGGAAGACAGAGACCAGCCATCATCTTTCCCTCGACAGAGCTAACATCCCACCTG AGACTTTTCAGAAATCATCACAGTTGTCGGAGTTACCCCAAAAGCCACCACTTGGAGACCTGCCCCCAAA GCCTATGGAACTGGCCCCCAAGCCCCAAATTGGAGAGTTGCCGCCTAAGCCTGGAGAGCTGCCCCCTAAG CCCCAATTAGGTGACCTGCCCCCCAAGCCACAGCTCTCAGACTTACCTCCCAAGCCACAGATGAAGGACC TTCCCCCCAAGCCACAGCTGGGGGACCTGCTGGCAAAGTCTCAGGCTAGCGATTTGTCGGCCAAGGTGCA GCCACCCTCAGAGGTCACACAGAGGTCACACACTGGGGATCTGTCTCCAAATGTGCAGTCCAGAGATGCC ATCCAGAAGCAAGCATCTGAGGACTCCAACGACCTCACACCCACGCTGCCAGAGACACCCGTACCACTGC CCAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGTGTGAAGACCATTTATGACTGCCAGGCAGACAA TGACGATGAGCTCACATTTATTGAGGGTGAAGTGATCATTGTCACTGGGGAAGAGGACCAGGAGTGGTGG ATTGGGCATATCGAAGGACAGCCTGAAAGGAAGGGTGTCTTTCCAGTGTCCTTTGTTCACATCCTGTCTG AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201663 representing NM_001044245
Red=Cloning site Green=Tags(s) MRSSASRLSSFSSRDSLWNRMPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTA LQKVKKSVKAIYNSGQDHVQNEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLS HNVIFTLDSLLKGDLKGVKGDLKKPFDKAWKDYEMKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKE RRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQD EEKKQLTALRDLIKSSLQLDPKEVGGLYVPSRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGI RKVWQRRKCAVKNGILTISHATSNRQPAKLNLLTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYVTWI SVLTNSKEEALTMAFRGEQSTGENSLEDLTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECS GIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYV DHRFSRKTCASSSAKLNELLEAIKSRDLLALIQVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLH LVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKPECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDL LSQAKSGKFNPHVHVEYEWNLRQDEMDESDDDLDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFST PRDKQRLSYGAFTNQIFVSTSTDLPTSPTSEAPPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRSP PPPPGHKRTLSDPPSPLPHGPPNKGAIPWGNDVGPSSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLP QKVALRKTETSHHLSLDRANIPPETFQKSSQLSELPQKPPLGDLPPKPMELAPKPQIGELPPKPGELPPK PQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGDLLAKSQASDLSAKVQPPSEVTQRSHTGDLSPNVQSRDA IQKQASEDSNDLTPTLPETPVPLPRKINTGKNKVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWW IGHIEGQPERKGVFPVSFVHILSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001044245 |
| ORF Size | 3432 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001044245.1, NP_001037710.1 |
| RefSeq Size | 3435 bp |
| RefSeq ORF | 3435 bp |
| Locus ID | 314961 |
| UniProt ID | Q1AAU6 |
| MW | 127.1 kDa |
| Gene Summary | Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201663 | Asap1 (untagged ORF) - Rat ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (Asap1), (10 ug) |
CNY 18,430.00 |
|
| RR201663L3 | Lenti ORF clone of Asap1 (Myc-DDK-tagged ORF) - Rat ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (Asap1), (10 ug) |
CNY 19,950.00 |
|
| RR201663L4 | Lenti ORF clone of Asap1 (mGFP-tagged ORF) - Rat ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (Asap1), (10 ug) |
CNY 21,950.00 |
