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Ide (NM_013159) Rat Tagged ORF Clone
CAT＃: RR202674
- TrueORF®
Ide (Myc-DDK-tagged ORF) - Rat insulin degrading enzyme (Ide), (10 ug)
"NM_013159" in other vectors (3)
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CNY 16,440.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | INSDEGM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202674 representing NM_013159
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAACGGGCTCGTGTGGCTGCTGCACCCCGCGCTGCCCAGCACCTTGCACTCCATCCTCGGCGCTC GCCCGCCTCCCGTGAAGCGACTGTGTGGATTCCCAAAACAAATTTACAGCACAATGAATAATCCGGCCAT CCAGAGAATAGAAGACCATATTGTCAAGTCTCCTGAAGACAAACGGGAATATCGTGGACTAGAACTGGCC AATGGTATCAAAGTGCTTCTCATCAGTGATCCCACCACGGACAAGTCATCAGCAGCACTCGACGTGCACA TAGGGTCACTGTCAGACCCTCCAAATATTCCTGGCTTAAGCCATTTTTGTGAGCATATGCTGTTTTTGGG AACAAAGAAATATCCTAAGGAAAATGAATACAGCCAGTTTCTCAGTGAACATGCTGGGAGTTCAAATGCA TTTACCAGCGGAGAACACACCAATTATTATTTCGATGTTTCCCATGAACACTTGGAAGGAGCCCTGGACA GGTTTGCCCAGTTTTTCCTGTGCCCCTTGTTTGATGCAAGTTGTAAGGACAGAGAGGTGAACGCTGTCGA TTCAGAGCATGAAAAGAATGTGATGAATGATGCCTGGAGACTCTTCCAGCTGGAAAAGGCTACAGGGAAT CCCAAGCACCCCTTCAGCAAATTCGGGACAGGAAACAAATATACTCTAGAGACTCGACCCAACCAAGAAG GCATCGATGTAAGGGAAGAACTCTTGAAATTTCACTCTACGTATTATTCGTCCAATCTGATGGCGATTTG TGTTTTAGGTCGAGAATCTTTAGATGACCTGACTAATCTGGTGGTGAAGTTATTTTCTGAAGTAGAGAAT AAAAATGTCCCTTTGCCTGAATTTCCTGAGCACCCTTTCCAAGAAGAACATCTTAAACAACTTTATAAAA TAGTACCCATTAAGGATATTAGGAATCTTTATGTGACATTTCCCATACCAGACCTTCAACAGTACTACAA ATCCAATCCCGGTCATTATCTCGGTCATCTGATCGGGCATGAAGGTCCTGGAAGCCTGTTGTCAGAGCTC AAGTCAAAGGGCTGGGTAAACACCCTGGTTGGGGGACAGAAGGAAGGAGCCCGAGGTTTTATGTTTTTTA TCATTAATGTGGACTTAACTGAAGAAGGGTTATTACATGTTGAAGATATAATTTTGCACATGTTTCAATA CATTCAGAAGCTACGTGCTGAAGGACCTCAAGAATGGGTTTTCCAAGAGTGCAAGGACTTGAACGCTGTC GCTTTCAGGTTTAAAGATAAAGAGAGGCCACGAGGCTACACATCTAAGATTGCAGGGAAATTGCACTATT ATCCCCTAAATGGAGTGCTCACAGCTGAATATTTACTGGAAGAATTTAGACCCGACTTGATAGACATGGT TCTTGATAAACTCAGACCAGAAAATGTCCGGGTGGCAATAGTTTCTAAATCATTTGAAGGGAAAACTGAC CGCACAGAGCAGTGGTATGGAACCCAGTACAAGCAAGAAGCTATCCCAGAGGACGTCATTCAGAAATGGC AAAATGCTGACCTGAATGGGAAATTTAAACTTCCAACAAAGAATGAATTCATTCCTACAAATTTTGAGAT TTTGGCATTAGAAAAAGATGCAACACCATACCCTGCTCTTATTAAGGATACAGCCATGAGTAAGCTGTGG TTCAAACAAGATGATAAATTTTTCTTGCCGAAAGCTTGTCTCAACTTTGAATTTTTCAGCCCGTTTGCTT ATGTGGACCCCTTGCACTGTAACATGGCCTATTTGTACCTTGAACTCCTCAAAGACTCACTCAACGAGTA TGCATATGCAGCAGAGCTAGCAGGCCTGAGCTATGATCTCCAAAACACCATCTATGGGATGTATCTCTCA GTGAAAGGTTACAATGACAAACAGCCAATTTTGCTAAAGAAGATCACCGAGAAAATGGCTACTTTTGAGA TTGATAAAAAAAGATTTGAAATTATCAAAGAGGCGTACATGCGATCTCTTAATAATTTCCGGGCTGAGCA GCCTCACCAGCACGCCATGTACTACCTCCGTCTGCTGATGACTGAAGTGGCCTGGACCAAAGATGAGTTA AAAGAAGCCCTCGATGATGTGACCCTCCCCCGTCTTAAGGCCTTCATACCTCAGCTGCTGTCCCGGCTGC ATATTGAAGCCCTTCTCCATGGCAACATAACAAAGCAGGCTGCCTTAGGAGTTATGCAGATGGTAGAAGA CACCCTTATTGAGCATGCTCACACCAAACCTCTCCTTCCAAGTCAGCTAGTCCGGTACAGAGAAGTTCAG CTCCCCGACCGAGGATGGTTTGTTTACCAGCGGAGGAACGAAGTCCACAATAACTGTGGCATTGAGATTT ACTACCAGACAGACATGCAGAGCACCTCGGAGAACATGTTCCTGGAGCTCTTCTGCCAGATTATCTCTGA GCCTTGCTTCAACACTCTGCGTACCAAGGAGCAGCTTGGCTATATTGTCTTCAGTGGACCTCGTCGGGCC AACGGCATCCAGGGCTTGCGATTCATCATCCAGTCAGAAAAACCACCTCACTACCTGGAAAGCAGAGTGG AAGCCTTCTTGATCACCATGGAAAAGGCCATAGAGGACATGACAGAGGAGGCTTTCCAAAAACACATTCA GGCGTTAGCGATTCGCCGACTCGACAAACCAAAGAAACTCTCTGCAGAGTGCGCGAAGTACTGGGGGGAG ATCATCTCCCAGCAGTACAATTATGACAGAGATAACATAGAGGTTGCATATTTAAAGACACTCAGCAAGG ATGATATCATCAAATTCTACAAGGAAATGTTGGCTGTGGACGCACCAAGGAGACATAAAGTATCCGTCCA CGTTCTTGCCAGGGAAATGGATTCTTGTCCTGTGGTTGGAGAGTTCCCCTCTCAGAATGATATAAACCTT TCCGAAGCGCCACCCTTGCCACAACCTGAGGTGATTCATAACATGACTGAATTCAAGCGCGGCCTGCCGC TGTTCCCCCTTGTGAAGCCACACATTAACTTCATGGCGGCAAAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202674 representing NM_013159
Red=Cloning site Green=Tags(s) MRNGLVWLLHPALPSTLHSILGARPPPVKRLCGFPKQIYSTMNNPAIQRIEDHIVKSPEDKREYRGLELA NGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNA FTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGN PKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVEN KNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSEL KSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAV AFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTD RTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILALEKDATPYPALIKDTAMSKLW FKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLS VKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDEL KEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQ LPDRGWFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRA NGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGE IISQQYNYDRDNIEVAYLKTLSKDDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINL SEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013159 |
| ORF Size | 3057 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013159.1, NP_037291.1 |
| RefSeq Size | 4276 bp |
| RefSeq ORF | 3060 bp |
| Locus ID | 25700 |
| UniProt ID | P35559 |
| MW | 117.7 kDa |
| Gene Summary | enzyme involved in the degradation of bioactive peptides [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202674 | Ide (untagged ORF) - Rat insulin degrading enzyme (Ide), (10 ug) |
CNY 16,440.00 |
|
| RR202674L3 | Lenti ORF clone of Ide (Myc-DDK-tagged ORF) - Rat insulin degrading enzyme (Ide), (10 ug) |
CNY 18,050.00 |
|
| RR202674L4 | Lenti ORF clone of Ide (mGFP-tagged ORF) - Rat insulin degrading enzyme (Ide), (10 ug) |
CNY 19,760.00 |
