United States
Germany
Japan
United Kingdom
China
Mef2a (NM_001014035) Rat Tagged ORF Clone
CAT＃: RR204477
- TrueORF®
Mef2a (Myc-DDK-tagged ORF) - Rat myocyte enhancer factor 2a (Mef2a), (10 ug)
"NM_001014035" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 4,024.00
CNY 6,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204477 representing NM_001014035
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGAAAGAAAATACAAATTACACGCATAATGGATGAGAGGAACCGACAGGTGACTTTTACAAAGC GAAAGTTCGGATTGATGAAGAAAGCCTATGAACTCAGTGTGCTCTGTGACTGTGAGATAGCACTCATCAT CTTCAACAGCTCTAACAAGTTGTTTCAGTACGCCAGCACTGACATGGACAAAGTCCTTCTCAAGTACACC GAGTACAACGAGCCTCATGAAAGCAGGACGAACTCGGATATTGTGGAGGCTCTGAACAAGAAGGAACACA GAGGCTGCGACAGCCCAGACCCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTGGCTTGCCGCCTCAGAACTTC TCAATGTCTGTCACAGTCCCTGTGACCAGCCCTAATGCTTTGTCGTACACTAACCCAGGGAGTTCACTCG TGTCACCGTCTTTGGCAGCCAGCTCAACATTAGCAGAGTCCAGCATGCTGTCTCCACCCCCAGCCACGCT ACATAGAAATGTGTCTCCAGGAGCTCCGCAGAGACCACCAAGCACTGGCAGTGCAGGTGGGATGTTGAGC ACTACAGACCTCACGGTACCAAATGGAGCTGGAAATAGTCCTGTGGGGAATGGATTTGTAAACTCAAGGG CCTCTCCAAATTTGATTGGAAATACTGGTGCGAATAGTGTAGGCAAAGTCATGCCTACAAAGTCCCCCCC GCCACCAGGTGGGGGCAGTGTTGGAATGAACAGTCGGAAACCAGATCTACGGGTTGTCATTCCCCCTTCA AGCAAGGGCATGATGCCCCCACTTAATGCCCAAAGGATAAGTAGTTCTCAAGCCACACAACCTCTTGCCA CGCCCGTGGTGTCTGTGACAACTCCAAGCTTGCCTCCGCAGGGACTAGTGTACTCGGCCATGCCGACAGC CTACAACACTGATTACTCATTGACCAGCGCTGACCTGTCCGCCCTGCAAGGCTTTACTTCCCCTGGAATG CTGTCTCTGGGGCAGGCTTCAGCCTGGCAGCAGCACCATCTAGGACAAGCGGCCCTCAGCTCTCTAGTGG CTGGAGGGCAGTTATCTCAGGGTTCAAATTTGTCCATTAACACCAACCAGAACATCAACATTAAGTCAGA GCCAATTTCACCTCCCCGTGATAGAATGACCCCATCAGGCTTCCAGCAGCAGCAGCAGCAGCAGCCACAG CAGCAACCACCGCCACAACCTCCACAGCCCCAGCCCCAACCCCGGCAGGAAATGGGGCGCTCCCCTGTGG ACAGTCTGAGCAGCTCTAGCAGCTCCTACGATGGCAGTGACCGGGAGGATCCACGGGGCGACTTCCATTC TCCAATTGTGCTTGGCCGACCTCCAAATGCTGAGGACAGAGAAAGCCCCTCTGTAAAACGCATGAGGATG GACACATGGGTGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204477 representing NM_001014035
Red=Cloning site Green=Tags(s) MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYT EYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNF SMSVTVPVTSPNALSYTNPGSSLVSPSLAASSTLAESSMLSPPPATLHRNVSPGAPQRPPSTGSAGGMLS TTDLTVPNGAGNSPVGNGFVNSRASPNLIGNTGANSVGKVMPTKSPPPPGGGSVGMNSRKPDLRVVIPPS SKGMMPPLNAQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFTSPGM LSLGQASAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQQQPQ QQPPPQPPQPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNAEDRESPSVKRMRM DTWVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014035 |
| ORF Size | 1485 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014035.1, NP_001014057.1 |
| RefSeq Size | 3237 bp |
| RefSeq ORF | 1488 bp |
| Locus ID | 309957 |
| UniProt ID | Q2MJT0 |
| MW | 53.3 kDa |
| Gene Summary | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204477 | Mef2a (untagged ORF) - Rat myocyte enhancer factor 2a (Mef2a), (10 ug) |
CNY 6,370.00 |
|
| RR204477L3 | Lenti ORF clone of Mef2a (Myc-DDK-tagged ORF) - Rat myocyte enhancer factor 2a (Mef2a), (10 ug) |
CNY 8,270.00 |
|
| RR204477L4 | Lenti ORF clone of Mef2a (mGFP-tagged ORF) - Rat myocyte enhancer factor 2a (Mef2a), (10 ug) |
CNY 9,030.00 |
