Rptor (NM_001134499) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1311784
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206228 representing NM_001134499 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCGGAGATGCTGCAGTCGCCTCTTATGGGACTCGGGGAGGAAGATGAGGCAGACCTTACAGATT GGAACTTGCCTTTGGCGTTTATGAAAAAGAGACATTGTGAGAAAATTGAAGGCTCCAAATCTTTAGCACA GAGCTGGAGAATGAAGGATCGGATGAAGACCGTCAGTGTGGCCTTGGTCCTGTGCCTGAATGTGGGTGTG GATCCTCCAGATGTGGTGAAGACCACACCTTGTGCTCGATTGGAATGCTGGATTGATCCTCTGTCTATGG GTCCTCAGAAAGCTCTGGAAACCATCGGTGCAAATCTACAGAAGCAGTATGAGAACTGGCAGCCAAGGGC TCGGTACAAGCAGAGCCTTGACCCCACTGTGGATGAAGTCAAGAAACTTTGCACATCTCTGCGTCGGAAC GCCAAGGAGGAACGGGTCCTCTTCCACTATAATGGCCATGGGGTGCCCAGGCCTACGGTGAATGGAGAGG TCTGGGTCTTCAACAAGAACTACACTCAGTACATCCCACTGTCCATATATGACCTGCAGACGTGGATGGG CAGCCCATCCATCTTTGTCTACGACTGCTCCAATGCTGGCCTCATCGTCAAGTCCTTCAAACAGTTCGCA CTGCAGAGGGAGCAGGAACTAGAGGTAGCTGCAATTAACCCAAACCATCCACTTGCCCAGATGCCCTTGC CTCCCTCAATGAAAAACTGCATCCAGCTGGCAGCCTGTGAGGCCCACGAGCTCCTGCCGATGATCCCTGA CCTCCCAGCCGACCTGTTCACATCCTGCCTCACCACTCCCATCAAGATTGCTTTGCGCTGGTTTTGCATG CAGAAGTGTGTTAGTCTGGTGCCTGGAGTCACACTGGATTTGATAGAAAAGATCCCTGGCCGGCTGAATG ACCGGAGAACCCCTCTGGGTGAACTGAACTGGATCTTCACAGCCATCACAGACACCATCGCCTGGAATGT GCTCCCTCGGGATCTCTTTCAAAAGCTTTTCCGACAGGACCTGCTGGTGGCAAGTTTGTTTAGAAATTTT TTATTGGCAGAAAGAATCATGAGGTCGTATAACTGCACCCCAGTAAGCAGCCCACGACTTCCCCCAACAT ACATGCATGCCATGTGGCAGGCCTGGGACCTTGCTGTGGACATTTGCCTCTCTCAACTGCCAACCATCAT CGAAGAGGGAACTGCCTTCAGGCACAGCCCATTCTTTGCTGAGCAGCTGACGGCATTCCAGGTGTGGCTC ACCATGGGTGTGGAAAACAGGAGCCCCCCCGAGCAGCTGCCCATCGTCCTACAGGTGCTGCTAAGCCAGG TGCACCGGCTGAGAGCCTTGGACTTGCTGGGACGATTTTTGGACTTGGGCCCTTGGGCGGTGAGCTTGGC CCTGTCGGTGGGCATCTTCCCCTATGTACTGAAGCTGCTTCAGAGCTCGGCCCGAGAGCTGCGGCCACTC CTTGTCTTCATCTGGGCCAAAATCCTCGCTGTGGACAGTTCATGCCAGGCTGACCTCGTGAAGGACAACG GTCACAAGTACTTCCTGTCCGTCTTGGCAGACCCATACATGCCAGCTGAACACAGGACCATGACAGCCTT CATTCTTGCTGTAATCGTCAACAGCTACACAACAGGGCAAGAAGCCTGCCTGCAGGGGAACCTGATTGCC ATCTGTTTGGAGCAGCTCAGTGACCCACACCCCTTGCTACGCCAGTGGGTGGCCATTTGCCTGGGAAGGA TCTGGCAGAACTTTGATTCTGCAAGGTGGTGTGGGGTGAGAGACAGCGCCCACGAAAAACTCTATAGCCT CCTCTCCGACCCCATCCCTGAGGTACGATGTGCAGCTGTGTTTGCCCTTGGTACCTTTGTGGGAAACTCT GCTGAGAGGACAGACCACTCTACTACCATTGACCACAATGTGGCCATGATGCTGGCTCAGCTGATTAATG ATGGAAGCCCCATGGTCCGGAAGGAGCTGGTGGTGGCTCTGAGTCACCTTGTAGTCCAATATGAGAGCAA TTTCTGCACTGTGGCCCTGCAGTTCATGGAAGAGGAAAAGAACTACCCCTTGCCTTCTCCAGCAGCCACA GAGGGAGGGAGCTTGACTCCAGTTCGAGACAGCCCGTGCACCCCCAGACTCCGGTCAGTGAGCTCCTATG GGAACATCCGAGCCGTCACCACAGCTAGGAACCTGAACAAATCTTTGCAGAACCTGAGCTTGACGGAAGA ATCGGGCAGCTCAGTGGCCTTCTCTCCCGGGAACCTCAGTACAAGTAGTAGTGCCAGCAGCACCTTGGGA AGCCCTGAGAACGAGGAGTATATCCTGTCCTTCGAGACCATCGACAAGATGCGGCGTGTGAGCTCCTATT CAGCGCTCAACTCCCTCATAGGAGTCTCCTTTAATAGTGTCTACACTCAGATTTGGAGAGTCTTACTGCA TTTGGCCGCTGATCCCTACCCAGATGTTTCTGACTTAGCCATGAAAGTCCTCAACAGCATTGCTTACAAG GCCACAGTCAACGCCCGGCCCCAGCGAATCCTCGACACATCCTCTCTCACACAGTCGGCCCCAGCCAGCC CAACCAACAAGGGCATGCACATCCACCAGGTGGGAGGCTCCCCGCCAGCATCTAGCACCAGCAGCTGCAG CCTGACCAATGATGTGGCCAAGCAGACGGTCAGCCGTGACCTGCCCTCCAGCCGCCCGGGTACTGCCGGC CCCACAGGGGCACAGTACACCCCTCACTCCCACCAGTTCCCTCGTACACGAAAGATGTTTGACAAAGGCC CTGATCAGACCACAGACGATGCAGATGATGCTGCCGGACACAAGAGCTTCATTTGTGCCACTATGCAGAC GGGGTTCTGTGACTGGAGTGCCCGCTACTTTGCCCAGCCGGTCATGAAGATCCCTGAAGAGCATGACCTA GAGAGTCAGATCCGCAAGGAGCGTGAGTGGCGGTTCCTGAGGAACACTCGAGTCAGGAAGCAGGCACAGC AGGTCATCCAGAAGGGCATCACCAGACTGGATGATCAGATATTTCTGAACAGGAACCCCGGCGTTCCCTC TGTGGTCAAATTCCACCCCTTTACACCATGCATAGCTGTTGCTGACAAGGACAGCATCTGTTTTTGGGAC TGGGAGAAAGGAGAGAAACTGGACTACTTCCACAATGGTAACCCTCGGTACACCAGAGTCACTGCTATGG AGTACCTGAATGGGCAGGACTGCTCCTTGCTGCTGACAGCCACAGACGATGGAGCCATCAGGGTCTGGAA AAATTTTGCTGATTTGGAAAAGAATCCAGAGATGGTGACTGCGTGGCAAGGGCTCTCAGACATGCTTCCA ACAACACGAGGAGCTGGGATGGTGGTGGACTGGGAACAAGAAACTGGCCTCCTCATGAGCTCAGGGGACG TGCGCATTGTCCGGATCTGGGATACCGACCGGGAGACGAAGGTCCAGGACATCCCCACGGGGGCCGACAG CTGTGTGACAAGTTTATCTTGTGATTCGCACCGCTCACTCATTGTAGCTGGCCTGGGTGATGGCTCGATC CGTGTCTACGACAGGAGGATGGCACTCAGTGAATGCCGGGTCATGACTTACCGAGAGCACACAGCATGGG TGGTAAAGGCCTACCTGCAGAAGCACCCTGAGGGCCACATCGTGAGTGTGAGTGTCAATGGAGATGTGCG ATTCTTCGATCCTCGGATGCCTGAGTCTGTGAATGTAATGCAGATTGTGAAGGGGCTGACAGCCCTCGAC ATCCACCCCCAGGCAAACCTGATCGCCTGTGGCTCCATGAACCAGTTCACAGCCATCTACAACGGCAATG GGGAGCTGATCAACAACATCAAATACTACGATGGCTTCATGGGCCAGCGAGTCGGGGCCATCAGCTGCCT GGCTTTCCACCCACACTGGCCTCATCTGGCCGTCGGAAGCAATGACTACTACATTTCTGTATATTCGGTG GAGAAGCGTGTCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206228 representing NM_001134499 Red=Cloning site Green=Tags(s) MESEMLQSPLMGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGV DPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRN AKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFA LQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEAHELLPMIPDLPADLFTSCLTTPIKIALRWFCM QKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNF LLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL TMGVENRSPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPL LVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYTTGQEACLQGNLIA ICLEQLSDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLYSLLSDPIPEVRCAAVFALGTFVGNS AERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPAAT EGGSLTPVRDSPCTPRLRSVSSYGNIRAVTTARNLNKSLQNLSLTEESGSSVAFSPGNLSTSSSASSTLG SPENEEYILSFETIDKMRRVSSYSALNSLIGVSFNSVYTQIWRVLLHLAADPYPDVSDLAMKVLNSIAYK ATVNARPQRILDTSSLTQSAPASPTNKGMHIHQVGGSPPASSTSSCSLTNDVAKQTVSRDLPSSRPGTAG PTGAQYTPHSHQFPRTRKMFDKGPDQTTDDADDAAGHKSFICATMQTGFCDWSARYFAQPVMKIPEEHDL ESQIRKEREWRFLRNTRVRKQAQQVIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCIAVADKDSICFWD WEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLP TTRGAGMVVDWEQETGLLMSSGDVRIVRIWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSI RVYDRRMALSECRVMTYREHTAWVVKAYLQKHPEGHIVSVSVNGDVRFFDPRMPESVNVMQIVKGLTALD IHPQANLIACGSMNQFTAIYNGNGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSV EKRVR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001134499
ORF Size	4005 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001134499.2, NP_001127971.2
RefSeq Size	6699 bp
RefSeq ORF	4008 bp
Locus ID	287871
MW	149.5 kDa

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