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Top1 (NM_022615) Rat Tagged ORF Clone
CAT＃: RR206407
- TrueORF®
Top1 (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) I (Top1), (10 ug)
"NM_022615" in other vectors (3)
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CNY 9,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ab2-086 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206407 representing NM_022615
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGACCATCTCCACAACGACTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAAC ACAAAGATAAACACAAAGATCGAGAACACCGGCATAAGGAGCACAAGAAGGATAAGGATAAGGACCGGGA AAAGTCTAAACATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAAGAGAAAACCAAA CACAAAGATGGAAGCTCAGACAAACATAAAGACAAACATAAAGACAGAGACAAGGAAAAACGAAAGGAGG AAAAGATTAGAGCTGCTGGGGATGCAAAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACG AATTAAAGACGAGCCTGAAGATGATGGCTATTTTGCTCCTCCAAAAGAGGATATAAAGCCACTGAAGAGG CCTCGGGATGAAGATGATGCTGATTATAAGCCTAAGAAAATTAAGACAGAAGATATCAAGAAGGAGAAGA AACGAAAACTAGAAGAAGAAGAGGATGGCAAACTTAAAAAGCCCAAGAATAAAGATAAAGATAAAAAGGT TGCTGAGCCAGATAATAAGAAGAAGAAGGCGAAAAAGGAAGAGGAGCAGAAGTGGAAATGGTGGGAAGAA GAACGCTATCCAGAAGGCATCAAGTGGAAATTCCTAGAACATAAAGGGCCTGTTTTCGCTCCACCGTATG AGCCTCTGCCAGAGGGTGTCAAGTTTTACTATGATGGTAAAGTTATGAAGCTGAGTCCCAAAGCAGAAGA AGTAGCTACATTCTTTGCAAAAATGCTTGACCATGAATATACTACTAAGGAAATATTCAGGAAAAATTTC TTTAAAGACTGGAGAAAGGAAATGACTAATGATGAAAAAAATACGATTACCAACCTAAGCAAATGCGACT TTACACAGATGAGCCAGTACTTCAAAGCTCAATCAGAAGCTCGGAAACAGATGAGCAAGGAAGAAAAATT GAAAATCAAAGAAGAAAATGAAAAATTATTGAAAGAATATGGCTTTTGTGTAATGGATAATCATAGAGAG CGGATTGCCAACTTCAAGATAGAGCCTCCAGGGCTCTTCCGAGGCCGTGGCAACCACCCCAAGATGGGTA TGCTGAAGAGAAGGATCATGCCTGAGGACATCATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCC TCCTCCTGGACATAAATGGAAGGAAGTTCGACATGATAACAAGGTTACTTGGCTGGTCTCCTGGACAGAG AATATCCAAGGTTCTATCAAATATATCATGCTGAATCCCAGTTCACGAATCAAGGGTGAGAAAGACTGGC AGAAGTATGAGACTGCTCGGCGACTGAAGAAGTGTGTGGACAAGATCCGAAATCAGTATCGGGAAGACTG GAAGTCCAAAGAAATGAAAGTTCGGCAGAGAGCTGTAGCACTGTACTTCATTGATAAGCTTGCTCTGAGA GCAGGCAATGAGAAGGAAGAAGGAGAGACAGCAGACACTGTGGGTTGCTGTTCACTTAGAGTGGAACACA TCAATCTACACCCAGAATTGGATGGTCAGGAGTACGTGGTGGAGTTTGACTTTCCTGGGAAGGACTCAAT CAGATACTACAACAAAGTCCCAGTTGAGAAACGAGTTTTTAAAAACTTACAACTATTTATGGAGAACAAG CAGCCTGAGGATGATCTTTTTGATCGACTTAATACTGGTATTCTAAATAAGCATCTTCAAGATCTCATGG AGGGCTTGACTGCCAAGGTGTTCCGTACCTACAATGCCTCCATCACGCTGCAGCAGCAGCTTAAAGAGCT CACAGCCCCTGATGAGAATGTACCAGCAAAGATTCTATCTTATAACCGTGCCAATCGCGCTGTTGCAATT CTTTGTAACCACCAGAGGGCGCCACCAAAGACCTTTGAGAAGTCAATGATGAACTTGCAGTCTAAGATTG ATGCCAAGAAAGATCAGCTAGCAGATGCTCGAAAGGACCTGAAAAGCGCTAAGGCTGATGCCAAAGTCAT GAAGGATGCAAAGACCAAGAAGGTAGTAGAGTCAAAAAAGAAGGCTGTACAGAGATTAGAAGAGCAGCTG ATGAAGCTGGAGGTTCAAGCCACAGACCGAGAGGAGAACAAACAAATTGCCTTGGGGACCTCCAAACTCA ATTACCTGGACCCTAGGATCACAGTGGCTTGGTGCAAAAAATGGGGGGTCCCAATCGAGAAGATTTACAA CAAAACCCAGAGAGAGAAGTTTGCTTGGGCCATTGATATGACTGATGAGGACTACGAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206407 representing NM_022615
Red=Cloning site Green=Tags(s) MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDKDREKSKHSNSEHKDSEKKHKEKEKTK HKDGSSDKHKDKHKDRDKEKRKEEKIRAAGDAKIKKEKENGFSSPPRIKDEPEDDGYFAPPKEDIKPLKR PRDEDDADYKPKKIKTEDIKKEKKRKLEEEEDGKLKKPKNKDKDKKVAEPDNKKKKAKKEEEQKWKWWEE ERYPEGIKWKFLEHKGPVFAPPYEPLPEGVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNF FKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRE RIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTE NIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALR AGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENK QPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAI LCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARKDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQL MKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022615 |
| ORF Size | 2301 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022615.1, NP_072137.1 |
| RefSeq Size | 2304 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 64550 |
| UniProt ID | Q9WUL0 |
| MW | 90.8 kDa |
| Gene Summary | human homolog catalyzes the transient breakage and rejoining of DNA; plays a role in nucleotide excision repair [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206407 | Top1 (untagged ORF) - Rat topoisomerase (DNA) I (Top1), (10 ug) |
CNY 9,880.00 |
|
| RR206407L3 | Lenti ORF clone of Top1 (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) I (Top1), (10 ug) |
CNY 11,690.00 |
|
| RR206407L4 | Lenti ORF clone of Top1 (mGFP-tagged ORF) - Rat topoisomerase (DNA) I (Top1), (10 ug) |
CNY 12,830.00 |
