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Ddx1 (NM_053414) Rat Tagged ORF Clone
CAT＃: RR207166
- TrueORF®
Ddx1 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1), (10 ug)
"NM_053414" in other vectors (3)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207166 representing NM_053414
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTTCTCCGAAATGGGTGTTATGCCCGAGATTGCACAAGCCGTGGAGGAGATGGATTGGCTTC TACCCACTGATATCCAGGCAGAATCTATCCCATTGATCCTAGGAGGAGGTGATGTACTTATGGCTGCAGA AACGGGAAGTGGAAAAACTGGTGCATTTAGTATTCCAGTTATCCAGATAGTGTATGAGACTCTGAAAGAC CAACAGGAAGGAAAGAAAGGGAAAGCAACTATTAAAACTGGTGCTTCTGTGCTCAACAAGTGGCAGATGA ACCCATATGATAGAGGGTCTGCTTTTGCAATTGGGTCAGATGGTCTGTGTTGTCAGAGCAGAGAGGTGAA GGAGTGGCATGGGTGCAGAGCGACTAGAGGACTGCTGAGAGGGAAGCACTACTACGAAGTATCCTGTCAT GACCAAGGGCTGTGCAGAGTTGGGTGGTCCACCATGCAGGCTTCCTTAGACCTGGGTACTGACAAGTTTG GATTTGGCTTCGGTGGAACAGGAAAGAAATCTCATAATAAACAATTTGATAATTATGGAGAGGAATTCAC TATGCATGACACCATTGGATGTTACTTAGACATCGATAAAGGGCACGTGAAGTTCTCTAAGAATGGAAAA GACCTTGGTCTGGCATTTGAAATACCTGCACACATAAAAAACCAAGCCCTCTTCCCTGCCTGTGTTTTGA AGAATGCTGAATTGAAATTTAACTTTGGTGAAGAAGAATTTAAGTTTCCACCAAAAGATGGTTTTGTTGC TCTTTCCAAGGCCCCTGATAGTTACGTCGTCAAATCGCAGCACACAGGTAATGCACAGGTGTCACAAACA AAATTTCTCCCTAATGCTCCAAAAGCTCTTATTGTGGAACCCTCCAGAGAGTTAGCTGAGCAAACCTTGA ACAATGTGAAACAGTTTAAGAAGTACATCGATAATCCTAAATTGCGGGAGCTTCTCATAATTGGTGGCGT TGCAGCTCGGGATCAGCTCTCCGTTCTGGATAATGGGGTTGATATTGTCGTCGGTACTCCAGGAAGATTA GATGATTTGGTGTCAACTGGAAAGCTCAACTTGTCTCAAGTTAGATTCCTGGTCCTGGATGAAGCAGATG GGCTTCTCTCTCAAGGTTATTCTGACTTTATTAACAGGATGCACAACCAGATTCCTCAGATTACTTCTGA TGGGAAAAGACTTCAGGTGATTGTTTGCTCTGCTACTCTGCATTCTTTTGATGTAAAGAAACTATCTGAG AAGATAATGCATTTTCCCACGTGGGTGGATTTGAAAGGAGAAGATTCTGTTCCTGATACTGTGCATCATG TCGTTGTTCCTGTGAACCCAAAAACTGACAGGCTCTGGGAAAGGCTGGGGAAGAACCACATTCGAACCGA TGATGTCCATGCAAAGGATAACACAAGACCTGGTGCCAACAGTCCAGAGATGTGGTCTGAAGCTATTAAA ATACTGAAGGGGGAGTATGCTGTCCGAGCAATCAAGGAGCACAAGATGGATCAAGCAATTATCTTCTGTA GAACGAAAATTGACTGTGACAACTTGGAGCAGTACTTTATGCAGCAAGGAGGAGGACCTGATAAAAAAGG ACACCAATTCTCATGTGTATGTCTTCATGGTGACAGAAAGCCTCACGAGAGAAAGCAGAACTTGGAACGA TTTAAGAAAGGAGACGTAAGATTCCTGATTTGCACAGACGTAGCTGCCAGAGGAATTGACATCCACGGTG TTCCTTATGTTATTAATGTCACCCTGCCTGATGAAAAGCAGAATTATGTGCACCGGATTGGCAGAGTGGG GCGAGCTGAAAGGATGGGCCTGGCTATTTCCCTGGTGGCAACAGAGAAAGAAAAGGTTTGGTATCACGTA TGCAGCAACCGTGGGAAGGGATGCTATAACACTAGACTCAAGGAAGATGGCGGCTGTACTATCTGGTACA ATGAAATGCAGCTACTTTCTGAGATAGAAGAACACCTGAACTGTACCATTTCCCAGGTTGAGCCAGATAT AAAGGTTCCAGTGGATGAGTTTGATGGGAAAGTTACATATGGTCAGAAAAGAGCTGCTGGCGGTGGGAAC TATAAAGGCCATGTGGACATCCTGGCACCTACCGTTCAAGAGTTGGCTGCCCTTGAAAAGGAAGCACAGA CATCTTTCCTACACCTTGGCTACCTTCCCAACCAGCTGTTCAGAACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207166 representing NM_053414
Red=Cloning site Green=Tags(s) MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKD QQEGKKGKATIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATRGLLRGKHYYEVSCH DQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGK DLGLAFEIPAHIKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDSYVVKSQHTGNAQVSQT KFLPNAPKALIVEPSRELAEQTLNNVKQFKKYIDNPKLRELLIIGGVAARDQLSVLDNGVDIVVGTPGRL DDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQITSDGKRLQVIVCSATLHSFDVKKLSE KIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKNHIRTDDVHAKDNTRPGANSPEMWSEAIK ILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFMQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLER FKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHV CSNRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGN YKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053414 |
| ORF Size | 2220 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053414.1, NP_445866.1 |
| RefSeq Size | 2487 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 84474 |
| UniProt ID | Q641Y8 |
| MW | 82.5 kDa |
| Gene Summary | associated with estrogen-dependent endometrial adenocarcinoma in the BDII rat [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207166 | Ddx1 (untagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1), (10 ug) |
CNY 9,600.00 |
|
| RR207166L3 | Lenti ORF clone of Ddx1 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1), (10 ug) |
CNY 11,310.00 |
|
| RR207166L4 | Lenti ORF clone of Ddx1 (mGFP-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 1 (Ddx1), (10 ug) |
CNY 12,350.00 |
