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Ctcf (NM_031824) Rat Tagged ORF Clone
CAT＃: RR207558
- TrueORF®
Ctcf (Myc-DDK-tagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug)
"NM_031824" in other vectors (3)
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CNY 9,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207558 representing NM_031824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGTGAGGCGGTTGAAGCCATTGTGGAAGAGTCTGAAACTTTCATTAAAGGAAAGGAAAGAAAGA CTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCTTGCCACCTACCCCAGAACCAGACAGACGG GGGTGAGGTGGTCCAGGATGTCAACAGTAGCGTACAGATGGTAATGATGGAACAGCTGGACCCTACCCTT CTCCAAATGAAGACTGAAGTAATGGAGGGGACAGTGGCTCCTGAAGCAGAAGCTGCAGTGGACGATACCC AAATCATAACCCTGCAGGTTGTAAACATGGAGGAACAGCCCATTAATATAGGAGAACTTCAGCTTGTCCA AGTACCTGTTCCTGTGACTGTACCTGTTGCTACTACGTCAGTAGAAGAACTTCAGGGGGCTTATGAGAAT GAAGTGTCTAAAGAGGGCCTTGCAGAAAGTGAACCGATGATATGTCACACCTTACCTTTACCTGAAGGAT TTCAGGTGGTAAAAGTGGGGGCCAATGGAGAAGTGGAGACACTAGAGCAGGGGGAGCTTCCTCCTCAGGA AGACCCTAGCTGGCAAAAAGACCCAGACTATCAGCCACCAGCCAAAAAAACAAAGAAAACCAAAAAGAGT AAACTTCGTTACACAGAGGAGGGCAAAGACGTGGATGTGTCTGTGTATGATTTTGAGGAAGAACAGCAGG AAGGACTGCTGTCTGAGGTTAATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCGAAGCCAACAAAAAT TAAAAAAAAAGGTGTAAAGAAAACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGCAGAGCATTCA GAACAGTAACCCTCCTGAGGAATCATCTTAACACACACACAGGTACTCGTCCTCACAAGTGCCCCGACTG CGACATGGCTTTTGTGACCAGTGGAGAGTTGGTGCGGCATCGTCGTTATAAACACACTCATGAGAAACCA TTTAAGTGTTCCATGTGTGATTATGCCAGTGTAGAAGTCAGCAAATTAAAACGACACATTCGCTCTCATA CTGGAGAGCGCCCGTTCCAGTGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCA TATGAGAACCCATTCAGGTGAAAAACCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAGAGTGGT ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCATTGTCCCCACTGTGACA CTGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGAGAAAGCAGCATTCCTATATTGAGCAGGGCAA AAAATGTCGCTACTGTGATGCTGTGTTTCATGAGAGATATGCTCTTATCCAGCATCAAAAATCACACAAA AATGAGAAGCGCTTCAAGTGCGACCAGTGTGATTATGCTTGTAGACAGGAGAGGCACATGATCATGCACA AGCGCACTCACACGGGGGAGAAGCCTTATGCCTGCAGCCACTGCGACAAGACCTTCCGCCAGAAACAGCT CCTTGACATGCATTTCAAGCGCTATCATGATCCCAACTTTGTCCCCGCTGCCTTTGTCTGTTCTAAGTGT GGGAAAACATTCACACGCCGGAACACAATGGCGAGACACGCAGATAACTGTGCTGGTCCAGACGGTGTAG AAGGGGAAAATGGAGGAGAGACAAAGAAGAGCAAACGAGGAAGAAAAAGAAAGATGCGGTCTAAAAAAGA AGACTCCTCTGACAGTGAAAATGCTGAGCCGGATCTCGATGACAATGAGGAGGAGGAGGAGCCTGCTGTA GAAATTGAACCTGAGCCAGAGCCGCAGCCGCAGCCTCAGCCACAGCCGCAGCCGCAGCCTGTGGCCCCAG CCCCACCACCTGCCAAGAAGAGAAGAGGGCGACCTCCTGGAAGAACCAACCAACCCAAACAGAACCAGCC AACAGCCATCATCCAGGTTGAAGACCAGAATACAGGTGCAATTGAGAACATTATAGTTGAAGTCAAAAAA GAGCCAGATGCCGAGCCTGCGGAGGGGGAAGAGGAGGAGGCTCAGGCAGCACCCGCAGACGCCCCCAACG GAGACCTCACGCCTGAGATGATTCTCAGCATGATGGACCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207558 representing NM_031824
Red=Cloning site Green=Tags(s) MEGEAVEAIVEESETFIKGKERKTYQRRREGGQEEDACHLPQNQTDGGEVVQDVNSSVQMVMMEQLDPTL LQMKTEVMEGTVAPEAEAAVDDTQIITLQVVNMEEQPINIGELQLVQVPVPVTVPVATTSVEELQGAYEN EVSKEGLAESEPMICHTLPLPEGFQVVKVGANGEVETLEQGELPPQEDPSWQKDPDYQPPAKKTKKTKKS KLRYTEEGKDVDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSN LDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCPDCDMAFVTSGELVRHRRYKHTHEKP FKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSG TMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSYIEQGKKCRYCDAVFHERYALIQHQKSHK NEKRFKCDQCDYACRQERHMIMHKRTHTGEKPYACSHCDKTFRQKQLLDMHFKRYHDPNFVPAAFVCSKC GKTFTRRNTMARHADNCAGPDGVEGENGGETKKSKRGRKRKMRSKKEDSSDSENAEPDLDDNEEEEEPAV EIEPEPEPQPQPQPQPQPQPVAPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKK EPDAEPAEGEEEEAQAAPADAPNGDLTPEMILSMMDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031824 |
| ORF Size | 2211 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031824.1, NP_114012.1 |
| RefSeq Size | 3708 bp |
| RefSeq ORF | 2214 bp |
| Locus ID | 83726 |
| UniProt ID | Q9R1D1 |
| MW | 83.9 kDa |
| Gene Summary | binds to and protects the H19 differentially methylated domain from de novo methylation during oocyte growth and is involved in normal preimplantation development [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207558 | Ctcf (untagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug) |
CNY 9,500.00 |
|
| RR207558L3 | Lenti ORF clone of Ctcf (Myc-DDK-tagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug) |
CNY 11,210.00 |
|
| RR207558L4 | Lenti ORF clone of Ctcf (mGFP-tagged ORF) - Rat CCCTC-binding factor (zinc finger protein) (Ctcf), (10 ug) |
CNY 12,350.00 |
