Il6st (NM_001008725) Rat Tagged ORF Clone

CAT#: RR210402

  • TrueORF®

Il6st (Myc-DDK-tagged ORF) - Rat interleukin 6 signal transducer (Il6st), (10 ug)



  "NM_001008725" in other vectors (3)

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CNY 10,080.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Ac1055; Gp130; Il-6rb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210402 representing NM_001008725
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCACTAAGGATCTGGCTAATGCAAGCTTTGCTTATTTTCCTCACTACTGAGTCTATAGGTCAAC
TTGTGGAACCATGTGGTTATATCTACCCTGAGTTTCCAGTTGTCCAGCGTGGCTCTAACTTCACTGCCAC
TTGTGTGCTAAAGGAGAAGTGTCTGCAGGTGTACTCCGTGAATGCCACTTACATCGTGTGGAAGACAAAC
CATGTTGCCGTTCCTAAGGAGCAGGTCACAGTCATCAACAGAACGGCATCCAGTGTCACATTCACAGACG
TGGTCTTTCAGAACGTCCAGCTCACCTGCAATATCCTGTCATTTGGACAGATCGAGCAGAATGTGTATGG
AATCACCATACTTTCAGGCTATCCTCCAGATATACCTACAAATTTGAGTTGCATTGTGAATGAGGGGAAG
AACATGCTGTGTCAGTGGGACCCAGGAAGGGAGACATACCTTGAAACAAACTACACTTTGAAGTCAGAGT
GGGCAACAGAGAAGTTTCCTGATTGTCGAACAAAGCATGGCACGAGCTCCTGCATGATGGGCTATACCCC
CATCTATTTTGTCAACATCGAGGTCTGGGTGGAGGCCGAGAATGCCCTTGGGAATGTCTCCTCAGAGCCT
ATCAATTTTGACCCCGTGGATAAAGTGAAACCCAGCCCACCTCATAATTTGTCAGTGACCAACTCAGAAG
AATTATCCAGTATATTAAAACTAGCATGGGTCAATTCAGGTTTGGACAGTATTTTAAGGCTGAAGTCGGA
CATCCAATATAGGACCAAAGATGCCTCAACTTGGATTCAGGTCCCTCTTGAAGATACAGTGTCTCCCCGG
ACTTCGTTCACTGTTCAGGATCTCAAGCCTTTTACAGAATATGTGTTTAGGATTCGGTCCATTAAGGAGA
ATGGGAAGGGCTACTGGAGTGACTGGAGTGAGGAGGCGAGCGGGACCACATATGAAGACAGACCATCCAA
AGCGCCAAGTTTCTGGTATAAGGTAAATGCAAACCATCCACAGGAGTATAGGTCTGCACGGCTCATATGG
AAGACATTGCCCCTTTCTGAAGCCAATGGGAAGATCTTGGATTATGAAGTGGTTCTTACACAGTCCAAGT
CAGTTTCGCAAACTTACACAGTTAACGGCACAGAGTTGATAGTAAACCTCACCAATAACCGCTATGTAGC
GTCTCTAGCAGCAAGAAATGTGGTCGGCAAGTCCCCTGCAACCGTCCTCACCATCCCCGGCTCCCACTTC
AAAGCTTCTCACCCCGTAGTGGATCTTAAAGCATTTCCAAAAGATAACCTGCTCTGGGTAGAATGGACAC
CGCCATCTAAACCTGTGAACAAATACATACTAGAGTGGTGTGTGTTGTCAGAGAACTCACCCTGCATCCC
AGACTGGCAGCAAGAAGATGGCACTGTGAATCGGACCCACTTAAGAGGAAGCCTACTGGAGAGCAAGTGC
TATCTGATCACAGTAACCCCAGTGTTTCCCGGTGGGCCTGGAAGCCCTGAGTCCATGAAGGCGTACCTCA
AACAAGCAGCTCCTTCTAAAGGACCGACTGTTCGGACAAAGAAAGTGGGGAAAAATGAAGCTGTCTTAGA
GTGGGACCATCTTCCTGTTGATGTCCAGAACGGATTCATTAGAAACTACTCCATATCTTATAGAACCAGT
GTTGGAAAAGAAATGGTTGTGCGTGTGGATTCTTCTCACACAGAATACACACTGTCCTCTTTGAGTAGCG
ATACACTGTACATGGTCCACATGGCAGCATACACAGAAGAAGGTGGGAAGGATGGGCCGGAATTCACTTT
TACAACACTAAAGTTTGCTCAAGGAGAAATCGAAGCCATAGTCGTGCCTGTGTGCTTAGCTTTCCTCCTG
ACAACGCTGCTGGGAGTCTTGTTCTGCTTTAATAAACGAGACCTAATTAAAAAACACATCTGGCCGAATG
TCCCAGATCCTTCAAAGAGTCATATTGCCCAGTGGTCACCTCACACCCCCCCAAGGCACAATTTTAACTC
CAAAGATCAGATGTACTCAGATGCCAATTTCACTGACGTAAGCGTTGTGGAAATAGAAGCAAACAACAAA
AAGCCTTGTCCAGATGACCTGAAATCCTTGGACCTGTTCAAGAAGGAGAAAATAAGTACAGAAGGGCACA
GCAGTGGCATTGGGGGGTCCTCGTGCATGTCCTCTTCTAGGCCCAGCATCTCCAGCAGTGAGGAGAACGA
GTCTGCTCAGAGCACCGCAAGCACGGTGCAGTACTCCACCGTGGTGCACAGCGGCTACAGGCACCAGGTG
CCGTCAGTGCAAGTGTTCTCAAGGTCTGAGTCCACCCAGCCCCTGCTAGACTCCGAGGAGCGGCCAGAGG
ACCTGCAGCTGGTGGATAGTGTGGACAGCGGTGACGAGATCTTACCCAGGCAACAGTATTTCAAACAAAG
CTGCAGTCAGCCTGGAGCCAGTCCAGATGTTTCACACTTCGAAAGGTCAAGCCAGGTTCCGTCAGGCAGT
GAGGAGGATTTTGTCAGACTGAAACAGCAGCAGGTTTCAGATCACATTTCAGAGCCCTATGGCTCCGAGC
AACGGAGGCTGTTTCAGGAAGGTTCTGTGGCAGATGCTCTTGGCACAGGGACTGATGGACAGATAGAGAG
ATTCGAATCTGTTGGAATGGAGACAGCGATGGATGAAGACATTTCCAAAAGTTACTTGCCACAGACTGTA
AGACAAGGTGGCTACATGCCACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210402 representing NM_001008725
Red=Cloning site Green=Tags(s)

MSALRIWLMQALLIFLTTESIGQLVEPCGYIYPEFPVVQRGSNFTATCVLKEKCLQVYSVNATYIVWKTN
HVAVPKEQVTVINRTASSVTFTDVVFQNVQLTCNILSFGQIEQNVYGITILSGYPPDIPTNLSCIVNEGK
NMLCQWDPGRETYLETNYTLKSEWATEKFPDCRTKHGTSSCMMGYTPIYFVNIEVWVEAENALGNVSSEP
INFDPVDKVKPSPPHNLSVTNSEELSSILKLAWVNSGLDSILRLKSDIQYRTKDASTWIQVPLEDTVSPR
TSFTVQDLKPFTEYVFRIRSIKENGKGYWSDWSEEASGTTYEDRPSKAPSFWYKVNANHPQEYRSARLIW
KTLPLSEANGKILDYEVVLTQSKSVSQTYTVNGTELIVNLTNNRYVASLAARNVVGKSPATVLTIPGSHF
KASHPVVDLKAFPKDNLLWVEWTPPSKPVNKYILEWCVLSENSPCIPDWQQEDGTVNRTHLRGSLLESKC
YLITVTPVFPGGPGSPESMKAYLKQAAPSKGPTVRTKKVGKNEAVLEWDHLPVDVQNGFIRNYSISYRTS
VGKEMVVRVDSSHTEYTLSSLSSDTLYMVHMAAYTEEGGKDGPEFTFTTLKFAQGEIEAIVVPVCLAFLL
TTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDANFTDVSVVEIEANNK
KPCPDDLKSLDLFKKEKISTEGHSSGIGGSSCMSSSRPSISSSEENESAQSTASTVQYSTVVHSGYRHQV
PSVQVFSRSESTQPLLDSEERPEDLQLVDSVDSGDEILPRQQYFKQSCSQPGASPDVSHFERSSQVPSGS
EEDFVRLKQQQVSDHISEPYGSEQRRLFQEGSVADALGTGTDGQIERFESVGMETAMDEDISKSYLPQTV
RQGGYMPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001008725
ORF Size 2754 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001008725.3, NP_001008725.2
RefSeq Size 7454 bp
RefSeq ORF 2757 bp
Locus ID 25205
MW 102.6 kDa
Gene Summary IL-6 signal transducing molecule [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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