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Wdr1 (NM_001014135) Rat Tagged ORF Clone
CAT＃: RR210806
- TrueORF®
Wdr1 (Myc-DDK-tagged ORF) - Rat WD repeat domain 1 (Wdr1), (10 ug)
"NM_001014135" in other vectors (3)
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CNY 7,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210806 representing NM_001014135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTACGAGATCAAGAAGGTGTTCGCCAGCCTCCCCCAGGTGGAGCGGGGTGTCTCTAAGATCCTCG GCGGAGACCCCAAGGGCGATAATTTCCTCTACACCAATGGCAAGTGCGTCATCCTGAGGAACATCGACAA CCCTGCTGTTGCTGACATCTACACAGAACACGCCCATCAGGTGGTAGTAGCCAAGTATGCACCCAGCGGA TTCTACATTGCTTCTGGAGATATCTCTGGGAAGCTAAGGATCTGGGATACCACACAGAAGGAGCACATTC TAAAGTATGAGTACCAGCCTTTCGCTGGGAAGATCAAGGACATAGCCTGGACCGAAGACAGTAAGAGGAT CGCCGTGGTCGGGGAAGGAAGGGAGAAATTTGGAGCTGTCTTCTTATGGGACACTGGCTCTTCTGTGGGT GAGATCACAGGACACAACAAAGTCATTAACAGCGTGGACATCAAGCAGAACAGGCCCTACCGACTAGCAA CAGGGAGTGACGATAACTGTGCTGCATTCTTCGAGGGCCCCCCATTCAAGTTCAAGTTTACAATTGGTGA CCACAGCCGCTTTGTCAACTGTGTGCGATTCTCTCCTGATGGGAACAGATTTGCCACAGCTAGTGCTGAT GGCCAGATTTTCATTTATGATGGGAAGACAGGAGAAAAGGTGTGTGCCCTTGGAGGAAGCAAGGCTCATG ATGGAGGAATTTATGCCATTAGTTGGAGTCCTGATAGCACCCATTTGCTATCTGCCTCTGGAGACAAAAC CTCTAAGATTTGGGATGTCAATGTAAACTCTGTGGTCAGCACGTTTCCCATGGGCTCCAATGTTCTGGAC CAGCAGCTGGGCTGCCTCTGGCAGAAGGACCACCTCCTTAGCATCTCCCTGTCTGGATACATCAACTACC TGGACAAAAATAACCCCAGCAAGCCCCTCCGGGTCATCAAGGGTCACAGTAAATCCATCCAGTGTCTGAC AGTGCACAAAAATGGTGGCAAATCCTACATCTATTCTGGGAGCCATGATGGCCATATTAATTACTGGGAT TCAGAGACAGGAGAGAATGACTCCTTCTCTGGAAAAGGCCACACAAATCAGGTGTCCAGGATGACTGTGG ACGAGTCTGGGCAGTTGGTCAGCTGCAGCATGGACGACACAGTGCGGTATACTAACCTCACACTGAGGGA CTACAGTGGACAAGGCGTTGTGAAACTGGATGTTCAGCCAAAGTGTGTAGCTGTTGGCCCTGGGGGATAT ACCGTGGTCGTGTGCATTGGACAGATTGTCCTTCTGAAGGACCAGAAGAAATGCTTCAGCATCGACAACC CTGGCTACGAGCCTGAAGTTGTTGCTGTGCACCCTGGTGGGGACACAGTAGCTGTTGGTGGCTCGGATGG CAATGTGCGTGTGTACTCCATCCTGGGTGCCACACTAAAGGATGAAGGCAAACTCCTAGAGGCCAAGGGG CCAGTGACAGACTTGGCCTACTCTCATGACGGTGCCTTCCTGGCTGTGTGTGATGCCAGCAAGGTGGTCA CAGTGTTCAGTGTAGCTGATGGCTATTCGGAGAATAATGTTTTTTATGGACACCATGCAAAAATTGTGTG CCTGGCCTGGTCACCAGATAATGAACACTTTGCCTCTGGCGGCATGGACATGATGGTATATGTGTGGACA CTGAGTGACCCAGAGACCAAAGTCAAGATTCAAGATGCCCACCGGCTGCACCACGTCAGCAGTCTGGCCT GGCTGGACGAACACACACTGGTCACCACCTCCCATGACGCCTCTGTGAAGGAGTGGACAATCACCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210806 representing NM_001014135
Red=Cloning site Green=Tags(s) MPYEIKKVFASLPQVERGVSKILGGDPKGDNFLYTNGKCVILRNIDNPAVADIYTEHAHQVVVAKYAPSG FYIASGDISGKLRIWDTTQKEHILKYEYQPFAGKIKDIAWTEDSKRIAVVGEGREKFGAVFLWDTGSSVG EITGHNKVINSVDIKQNRPYRLATGSDDNCAAFFEGPPFKFKFTIGDHSRFVNCVRFSPDGNRFATASAD GQIFIYDGKTGEKVCALGGSKAHDGGIYAISWSPDSTHLLSASGDKTSKIWDVNVNSVVSTFPMGSNVLD QQLGCLWQKDHLLSISLSGYINYLDKNNPSKPLRVIKGHSKSIQCLTVHKNGGKSYIYSGSHDGHINYWD SETGENDSFSGKGHTNQVSRMTVDESGQLVSCSMDDTVRYTNLTLRDYSGQGVVKLDVQPKCVAVGPGGY TVVVCIGQIVLLKDQKKCFSIDNPGYEPEVVAVHPGGDTVAVGGSDGNVRVYSILGATLKDEGKLLEAKG PVTDLAYSHDGAFLAVCDASKVVTVFSVADGYSENNVFYGHHAKIVCLAWSPDNEHFASGGMDMMVYVWT LSDPETKVKIQDAHRLHHVSSLAWLDEHTLVTTSHDASVKEWTITY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014135 |
| ORF Size | 1818 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014135.1, NP_001014157.1 |
| RefSeq Size | 2844 bp |
| RefSeq ORF | 1821 bp |
| Locus ID | 360950 |
| UniProt ID | Q5RKI0 |
| MW | 66.2 kDa |
| Gene Summary | Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance. Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension. Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210806 | Wdr1 (untagged ORF) - Rat WD repeat domain 1 (Wdr1), (10 ug) |
CNY 7,790.00 |
|
| RR210806L3 | Lenti ORF clone of Wdr1 (Myc-DDK-tagged ORF) - Rat WD repeat domain 1 (Wdr1), (10 ug) |
CNY 9,600.00 |
|
| RR210806L4 | Lenti ORF clone of Wdr1 (mGFP-tagged ORF) - Rat WD repeat domain 1 (Wdr1), (10 ug) |
CNY 10,550.00 |
