Bdnf (NM_012513) Rat Tagged ORF Clone
CAT#: RR210837
- TrueORF®
Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug)
"NM_012513" in other vectors (5)
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CNY 3,600.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC105254 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR210837 representing NM_012513
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTCGGTTGCA TGAAGGCTGCGCCCATGAAAGAAGCAAACGTCCACGGACAAGGCAACTTGGCCTACCCAGCTGTGCGGAC CCATGGGACTCTGGAGAGCGTGAATGGGCCCAGGGCAGGTTCGAGAGGTCTGACGACGACGTCCCTGGCT GACACTTTTGAGCACGTGATCGAAGAGCTGCTGGATGAGGACCAGAAGGTTCGGCCCAACGAAGAAAACC ATAAGGACGCGGACTTGTACACTTCCCGGGTGATGCTCAGCAGTCAAGTGCCTTTGGAGCCTCCTCTGCT CTTTCTGCTGGAGGAATACAAAAATTACCTGGATGCCGCAAACATGTCTATGAGGGTTCGGCGCCACTCC GACCCCGCCCGCCGTGGGGAGCTGAGCGTGTGTGACAGTATTAGCGAGTGGGTCACAGCGGCAGATAAAA AGACTGCAGTGGACATGTCCGGTGGGACGGTCACAGTCCTGGAGAAAGTCCCGGTATCAAAAGGCCAACT GAAGCAATATTTCTACGAGACCAAGTGTAATCCCATGGGTTACACGAAGGAAGGCTGCAGGGGCATAGAC AAAAGGCACTGGAACTCGCAATGCCGAACTACCCAATCGTATGTTCGGGCCCTTACTATGGATAGCAAAA AGAGAATTGGCTGGCGGTTCATAAGGATAGACACTTCCTGTGTATGTACACTGACCATTAAAAGGGGAAG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210837 representing NM_012513
Red=Cloning site Green=Tags(s) MFHQVRRVMTILFLTMVISYFGCMKAAPMKEANVHGQGNLAYPAVRTHGTLESVNGPRAGSRGLTTTSLA DTFEHVIEELLDEDQKVRPNEENHKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHS DPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGID KRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012513 |
ORF Size | 771 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012513.4, NP_036645.2 |
RefSeq Size | 4252 bp |
RefSeq ORF | 774 bp |
Locus ID | 24225 |
UniProt ID | P23363 |
MW | 29.6 kDa |
Gene Summary | plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Crossing the Blood-Brain-Barrier: A bifunctional liposome for BDNF gene delivery – A Pilot Study
,Diniz, D;Franze, S;Homberg, J;,
bioRxiv
[BDNF]
|
Noninvasive, targeted gene therapy for acute spinal cord injury using LIFU-mediated BDNF-loaded cationic nanobubble destruction
,Song, Z;Ye, Y;Zhang, Z;Shen, J;Hu, Z;Wang, Z;Zheng, J;,
Biochem. Biophys. Res. Commun.
,PubMed ID 29360450
[BDNF]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN210837 | Bdnf (untagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug) |
CNY 3,600.00 |
|
RR210837L1 | Lenti ORF clone of Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug) |
CNY 6,080.00 |
|
RR210837L2 | Lenti ORF clone of Bdnf (mGFP-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug) |
CNY 6,000.00 |
|
RR210837L3 | Lenti ORF clone of Bdnf (Myc-DDK-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug) |
CNY 6,080.00 |
|
RR210837L4 | Lenti ORF clone of Bdnf (mGFP-tagged ORF) - Rat brain derived neurotrophic factor (Bdnf), (10 ug) |
CNY 6,650.00 |