United States
Germany
Japan
United Kingdom
China
Map3k11 (NM_001013150) Rat Tagged ORF Clone
CAT＃: RR210995
- TrueORF®
Map3k11 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase 11 (Map3k11), (10 ug)
"NM_001013150" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 10,830.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mlk-3; Mlk3; RHOE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210995 representing NM_001013150
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTTGAAGAACCTCTTCCTCAAGAGCCCGCTGGGGTCATGGAACGGCAGTGGCAGTGGGGGCG GTGGGGGTTCTGGTGGAGTCCGGCCAGAGGGGTCCCCGAAGGCAACAGCAGCTTATGCCAACCCTGTCTG GACAGCCCTGTTCGACTATGAGCCCAATGGGCAGGACGAACTAGCCCTGAGGAAGGGCGACCGTGTGGAG GTGCTGTCCAGGGATGCAGCCATCTCAGGAGATGAAGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGG GCATTTTTCCATCCAACTATGTATCTCGGGGTGGAGGCCCACCCCCCTGTGAGGTGGCCAGCTTCCAGGA GCTGCGACTGGAGGAGGTGATCGGCATCGGTGGCTTCGGCAAGGTCTACCGTGGCAGCTGGCGAGGGGAG CTGGTGGCTGTGAAAGCAGCTCGACAGGATCCTGATGAGGACATCAGTGTAACAGCTGAGAGTGTTCGAC AGGAGGCCCGGCTTTTTGCCATGCTGGCACATCCCAACATCATTGCCCTCAAAGCTGTGTGCCTGGAGGA GCCAAACCTATGCCTGGTGATGGAGTATGCAGCTGGTGGACCTCTTAGCCGTGCCCTGGCTGGTCGGCGG GTGCCCCCCCATGTGCTGGTCAACTGGGCTGTGCAGATTGCCCGAGGGATGCACTACCTGCACTGTGAGG CTCTAGTGCCTGTGATACACCGAGACCTGAAGTCCAACAACATTCTGCTGCTGCAGCCCATTGAAGGTGA CGACATGGAGCACAAGACCCTAAAGATTACTGACTTTGGCCTCGCCCGAGAGTGGCACAAAACCACACAG ATGAGTGCTGCGGGGACCTATGCGTGGATGGCTCCTGAGGTTATCAAGGCATCTACCTTCTCCAAGGGCA GCGATGTCTGGAGCTTTGGGGTGCTGCTGTGGGAACTGCTGACTGGGGAGGTACCCTACCGTGGTATCGA CTGTCTTGCGGTAGCCTATGGTGTGGCTGTAAACAAGTTAACATTACCCATCCCATCCACTTGCCCTGAG CCCTTCGCACAACTCATGGCTGACTGCTGGGCACAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCC TGCAGCAGTTGGAAGCTCTAGAGGCTCAGGTGCTGAGGGAGATGCCACGGGACTCCTTCCATTCCATGCA GGAGGGCTGGAAACGCGAGATCCAGGGCCTTTTTGATGAGCTGCGGGCCAAGGAAAAGGAACTACTGAGC CGCGAGGAGGAACTGACGCGCGCAGCACGCGAGCAGCGGTCCCAGGCAGAGCAGCTGCGACGGCGCGAGC ACCTGCTGGCGCAGTGGGAGCTGGAGGTGTTCGAGCGCGAGCTGACACTGCTGCTACAGCAGGTGGACCG AGAACGGCCACACGTGCGCCGCCGACGCGGCACCTTCAAGCGGAGCAAGCTCCGAGCGAGGGATGGCGGA GAACGCATCAGCATGCCCCTGGACTTCAAACATCGCATCACTGTGCAGGCCTCTCCAGGACTTGACCGGA GGAGAAATGTGTTCGAGGTTGGGGCTGGGGACTCGCCCACCTTCCCTCGGTTCCGAGCTATCCAGTTGGA ACCTGCAGAATCAGGCCAGACTTGGGGTCGCCAGTCCCCTAGACGTCTTGACGATTCAAGCAATGGAGAG AGGCGTGCTTGCTGGGCCTGGGGTCCCAGTTCCCCCAAGCCTGGGGAGGCCCAGAATGGGAGGAGAAGGT CCCGCATGGATGAAGCCACGTGGTACCTAGATTCAGATGACTCATCCCCATTAGGGTCTCCTTCTACTCC CCCAGCACTCAACGGTAATCCTCCAAGGCCTAGCCCTGAGCCAGAGGAGCCACGGCGGTCTGGGCCCACA GAACGGGGCAATAGCTCTGGGACGCCTAAACTGATCCAACGTGCATTGCTCCGGGGGACCGCTCTGCTGG CTTCCCTAGGCCTTGGCCGAGACCTGCAGCCTCCAGGAGGCCTGAGCCGTGAACGTGGAGAGTCCCCGAC AGCACCACCCCCTGCACAGATGGCTTCACCCTGTCCACCTGACCTGCCCTCCACCCCGCTCATCCACCTC TCACAAGCAACACCTGATGCCCGTGGACCACTCACCCCTGCACCCTTATTGCTGGACCTAGGTGTTTCCT CTGGCCAGCCATCAGCCAAGAGCCCTCGGCGGGAAGAGACACGTGGAAGAACTGTTTCACCTCCACCAGG AATATCACGCTCTGCTCCTGGCACCCCTGGAACCCCTCGCTCACCACCTTTGGGCCTCATCAGCCGACCT CGGCCATCACCACTCCGTAACCGTATTGACCCATGGAGCTTCGTGTCAGCTGGGCCACGGCCTTCACCCC TGCCCTCTCCACAGCCTGCACCCCGACGGGCACCATGGACCTTATTCCCAGACTCAGATCCCTTCTGGGA CTCCCCACCTGCCAACCCCTTCCGGGGAGGCTCCCAGGACTGCAGGACGCAGACCAAAGATGTGGGTGCT CAGGCGCCATGGGCACCAGAAGCAGGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210995 representing NM_001013150
Red=Cloning site Green=Tags(s) MEPLKNLFLKSPLGSWNGSGSGGGGGSGGVRPEGSPKATAAYANPVWTALFDYEPNGQDELALRKGDRVE VLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGE LVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRR VPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIEGDDMEHKTLKITDFGLAREWHKTTQ MSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPE PFAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLS REEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERPHVRRRRGTFKRSKLRARDGG ERISMPLDFKHRITVQASPGLDRRRNVFEVGAGDSPTFPRFRAIQLEPAESGQTWGRQSPRRLDDSSNGE RRACWAWGPSSPKPGEAQNGRRRSRMDEATWYLDSDDSSPLGSPSTPPALNGNPPRPSPEPEEPRRSGPT ERGNSSGTPKLIQRALLRGTALLASLGLGRDLQPPGGLSRERGESPTAPPPAQMASPCPPDLPSTPLIHL SQATPDARGPLTPAPLLLDLGVSSGQPSAKSPRREETRGRTVSPPPGISRSAPGTPGTPRSPPLGLISRP RPSPLRNRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFRGGSQDCRTQTKDVGA QAPWAPEAGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013150 |
| ORF Size | 2550 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013150.1, NP_001013168.1 |
| RefSeq Size | 3536 bp |
| RefSeq ORF | 2553 bp |
| Locus ID | 309168 |
| UniProt ID | Q66HA1 |
| MW | 93.1 kDa |
| Gene Summary | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210995 | Map3k11 (untagged ORF) - Rat mitogen-activated protein kinase kinase kinase 11 (Map3k11), (10 ug) |
CNY 10,830.00 |
|
| RR210995L3 | Lenti ORF clone of Map3k11 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase 11 (Map3k11), (10 ug) |
CNY 12,730.00 |
|
| RR210995L4 | Lenti ORF clone of Map3k11 (mGFP-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase 11 (Map3k11), (10 ug) |
CNY 13,870.00 |
其它Map3k11产品
