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Plk1 (NM_017100) Rat Tagged ORF Clone
CAT＃: RR214157
- TrueORF®
Plk1 (Myc-DDK-tagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug)
"NM_017100" in other vectors (3)
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CNY 7,184.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Plk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214157 representing NM_017100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCAGCGGCCAAAGCTGGAAAGCTGGCTCGAGCACCAGCCGACCTCGGGAAAGGTGGGGTTCCGG GAGATGCAGTTCCCGGTGCCCCAGTGGCCGCCCCGCTGGCGAAAGAAATTCCGGAGGTCCTAGTGGACCC ACGCAGCCGGCAGCAGTATGTACGGGGCCGCTTCCTGGGTAAAGGAGGCTTCGCCAAATGCTTCGAAATC TCAGACTCAGACACAAAAGAGGTGTTCCCAGGCAAGATCGTGCCTAAGTCTTTGCTGCTCAAGCCCCATC AGAAGGAGAAAATGTCTATGGAGACCTCCATTCACCGCAGCCTCGAACACCAGCACGTCGTAGGCTTCCA TGGCTTTTTTGAGGACAGCGACTTTGTGTTCGTGGTTTTGGAGCTCTGTCGCAGGAGGTCTCTCCTGGAG CTGCACAAAAGGAGGAAGGCGCTGACCGAGCCCGAGGCCCGATACTACCTGCGGCAGATAGTCCTGGGTT GCCAGTACCTGCACCGCAATCAAGTCATTCACAGGGACCTCAAACTGGGCAACCTCTTTCTGAATGAGGA TCTGGAGGTGAAAATAGGGGATTTTGGCCTGGCAACAAAAGTGGAATATGAAGGGGAACGGAAGAAGACC CTGTGTGGCACTCCTAACTACATAGCTCCCGAGGTGCTGAGCAAGAAGGGACACAGCTTTGAGGTGGACG TGTGGTCCATTGGGTGCATCATGTATACCTTGCTGGTGGGCAAGCCACCTTTTGAGACCTCATGCCTAAA AGAGACCTACCTCCGGATCAAGAAAAATGAATACAGTATTCCCAAGCACATCAACCCAGTGGCTGCCTCC CTCATCCAGAAAATGCTTCAGACAGACCCCGCTGCGCGCCCCACCATTCACGAGTTGCTCAATGACGAGT TCTTCACTTCCGGATACATCCCCGCCCGTCTGCCTATTACCTGCCTCACCATCCCACCAAGGTTTTCAAT AGCTCCCAGCAGCCTAGACCCCAGCAACAGGAAGCCTCTCACAGTCCTTAATAAAGGTGTGGAGAACCCC CTGCCTGACCGTCCCCGAGAGAAAGAGGAACCAGTGGTTCGGGAGACAAATGAGGCCATCGAGTGCCACC TTAGTGACTTGCTACAGCAGCTGACCAGTGTCAACGCCTCCAAGCCCTCAGAGCGAGGGCTGGTGCGGCA AGAGGAGGCCGAGGATCCTGCTTGCATCCCTATCTTCTGGGTCAGCAAATGGGTAGACTATTCGGACAAG TATGGCCTTGGTTATCAGCTATGTGACAACAGTGTGGGAGTGCTCTTTAATGACTCAACACGCCTCATCC TCTACAATGACGGGGACAGCCTGCAGTACATAGAGCGTGATGGCACGGAGTCCTACCTCACTGTGAGCTC CCACCCCAACTCCTTGATGAAGAAGATCACTCTCCTCAACTATTTCCGCAATTACATGAGTGAACACCTG CTGAAGGCGGGGGCCAACATCACGCCCCGGGAAGGCGACGAGCTGGCCCGGCTGCCCTACCTACGAACAT GGTTCCGCACACGCAGCGCCATCATCCTGCACCTCAGCAATGGCACAGTACAGATTAATTTCTTCCAGGA CCACACCAAACTTATCCGTGGCCCCCTGATGGCAGCAGTGACCTACATCAACGAGAAGAGGGACTTCCGG ACATACCGCCTGAGCCTTCTGGAGGAATATGGCTGCTGCAAGGAACTGGCTAGCCGCCTCCGCTATGCCC GCACCATGGTAGACAAGCTGCTGAGCTCGCGTTCCGCCTGCAACCGCCTCAAGGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214157 representing NM_017100
Red=Cloning site Green=Tags(s) MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRQQYVRGRFLGKGGFAKCFEI SDSDTKEVFPGKIVPKSLLLKPHQKEKMSMETSIHRSLEHQHVVGFHGFFEDSDFVFVVLELCRRRSLLE LHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKT LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS LIQKMLQTDPAARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGVENP LPDRPREKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHL LKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLIRGPLMAAVTYINEKRDFR TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSACNRLKAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017100 |
| ORF Size | 1809 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017100.1, NP_058796.1 |
| RefSeq Size | 2198 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 25515 |
| UniProt ID | Q62673 |
| MW | 68.3 kDa |
| Gene Summary | human homolog catalyzes the phosphorylation of a Golgi reassembly stacking protein (GRASP65); may play a role in Golgi apparatus fragmentation and reorganization during mitosis [RGD, Feb 2006] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PLK1 is a critical determinant of tumor cell sensitivity to CPT11 and its inhibition enhances the drug antitumor efficacy in squamous cell carcinoma models sensitive and resistant to camptothecins
,null,
Oncotarget
,PubMed ID 25826089
[Plk1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214157 | Plk1 (untagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug) |
CNY 7,790.00 |
|
| RR214157L3 | Lenti ORF clone of Plk1 (Myc-DDK-tagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug) |
CNY 9,600.00 |
|
| RR214157L4 | Lenti ORF clone of Plk1 (mGFP-tagged ORF) - Rat polo-like kinase 1 (Drosophila) (Plk1), (10 ug) |
CNY 10,450.00 |
