Snx17 (NM_001011981) Rat Tagged ORF Clone

CAT#: RR215439

  • TrueORF®

Snx17 (Myc-DDK-tagged ORF) - Rat sorting nexin 17 (Snx17), (10 ug)



  "NM_001011981" in other vectors (3)

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CNY 6,080.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR215439 representing NM_001011981
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTTTTCCATTCCTGAAACCGAGTCCCGCAGCGGGGACAGCGGCGGCTCCACCTACGTGGCCTATA
ACATTCACGTGAATGGAGTCCTGCACTGTCGTGTGCGCTACAGCCAGCTCCTGGGGCTGCACGAGCAGCT
TCGGAAGGAGTATGGGGCCAATGTGCTTCCTGCATTCCCCCCAAAGAAGCTTTTCTCTTTGACGCCTGCA
GAGGTAGAACAGAGGAGGGAACAGTTAGAGAAGTACATGCAAGCTGTTCGGCAAGATCCATTGCTTGGGA
GCAGTGAGACCTTTAATAGCTTTCTACGTCGGGCCCAACAGGAGACACAACAGGTCCCCACAGAGGAGGT
TTCCTTGGAAGTGTTGCTCAGTGACGGACAGAAAGTTCTGGTCAATGTGCTAACTTCTGACCAGACGGAA
GACGTCCTGGAGGCCGTGGCTGCAAAGCTGGATCTTCCAGATGACTTGATCGGATATTTTAGTCTCTTCC
TTGTTCGAGAAAAAGAAGATGGCGCCTTTTCTTTTGTACGGAAGCTTCAGGAGTTTGAGCTGCCTTACGT
ATCTGTCACCAGTCTTCGGAGTCAAGAGTATAAGATCGTGCTCAGAAAGAGTTACTGGGACTCTGCCTAT
GACGACGATGTCATGGAGAACCGGGTTGGCCTGAACCTGCTTTATGCCCAGACGGTGTCAGACATCGAGC
ATGGCTGGATCCTGGTCACCAAGGAGCAGCACCGACAGCTCAAATCTCTGCAAGAGAAGGTCTCCAAAAA
GGAGTTCTTGCGGCTGGCCCAGACTCTGCGGCACTATGGCTATCTGCGCTTTGATGCCTGTGTGGCTGAC
TTTCCGGAGAAGGACTGCCCTGTTGTGGTCAGTGCAGGCAACAGTGAGCTCAGTCTCCAGCTGCGACTCC
CAGGCCAGCAGCTCCGTGAAGGCTCTTTCCGGGTCACCCGAATGCGGTGCTGGCGGGTCACCTCCTCTGT
GCCACTCCCCAGTGGGGGTACAAGCACTCCAAGCCGGGGTCGGGGTGAGGTACGCCTGGAACTGGCTTTT
GAATACCTCATGAGCAAGGACCGGCTACAGTGGGTCACCATCACCAGCCCACAGGCTATCATGATGAGCA
TCTGCCTGCAGTCCATGGTAGATGAGCTGATGGTGAAGAAGTCTGGGGGCAGTATCAGGAAGATGCTGCG
TCGGAGGGTGGGGGGCAACCTGAGACGTTCAGACAGCCAGCAAGCAGTGAAGTCCCCACCATTGCTCGAA
TCACCAGATGCCAGCCGGGAATCCATGGTCAAACTCTCAAGTAAACTGAGTGCGGTGAGCTTGCGGGGAA
TTGGCAGTCCCAGCACAGATGCCAGTGCCAGTGCCGTCCACGGCAATTTCGCCTTCGAGGGCATTGGAGA
TGAGGATCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR215439 representing NM_001011981
Red=Cloning site Green=Tags(s)

MHFSIPETESRSGDSGGSTYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPA
EVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSDGQKVLVNVLTSDQTE
DVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAY
DDDVMENRVGLNLLYAQTVSDIEHGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVAD
FPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGGTSTPSRGRGEVRLELAF
EYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGNLRRSDSQQAVKSPPLLE
SPDASRESMVKLSSKLSAVSLRGIGSPSTDASASAVHGNFAFEGIGDEDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001011981
ORF Size 1410 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001011981.1, NP_001011981.1
RefSeq Size 1944 bp
RefSeq ORF 1413 bp
Locus ID 298836
UniProt ID Q6AYS6
MW 52.9 kDa
Gene Summary Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits. Also required for maintenance of normal cell surface levels of APP and LRP1. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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