Nfkb1 (NM_001276711) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EBP-1; NF-kB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217379 representing NM_001276711 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACGACGATCCTTTCGGAACTGGGCAAATGTTTCATTTGAATACTGCTTTGACTCACTCCATAT TTAATGCAGAATTATATTCAACAGATATACCACTGTCAACAGATGGCCCATACCTTCAAATACTAGAGCA ACCGAAACAGAGAGGATTTCGATTCCGCTACGTGTGCGAAGGCCCGTCCCACGGAGGGCTTCCCGGTGCC TCCAGTGAGAAGAACAAGAAATCTTACCCGCAGGTCAAAATTTGCAACTATGTGGGGCCTGCAAAGGTTA TCGTTCAGTTGGTCACAAATGGAAAAAATATTCACCTGCACGCCCACAGCCTGGTGGGCAAGCACTGTGA GGACGGCATATGCACCGTGACAGCAGGACCCAAGGACATGGTGGTTGGCTTTGCAAACCTGGGAATACTT CACGTGACTAAGAAAAAGGTATTCGAAACACTGGAAGCACGGATGACAGAGGCGTGTATAAGGGGCTATA ACCCTGGACTTCTGGTGCATTCTGACCTTGCCTATCTACAGGCAGAAGGCGGAGGAGACCGGCAACTCAC AGACAGAGAGAAGGAGATCATCCGCCAGGCAGCTCTGCAGCAGACCAAGGAGATGGACCTGAGTGTGGTG CGCCTCATGTTCACAGCCTTCCTCCCCGACAGCACGGGCAGCTTCACTCGGAGACTGGAACCTGTGGTGT CAGACGCCATCTATGACAGCAAAGCCCCCAACGCGTCCAACCTGAAGATCGTAAGAATGGACAGAACAGC AGGCTGCGTGACGGGAGGGGAAGAAATCTATCTTCTCTGTGACAAGGTCCAGAAAGATGACATCCAGATT CGGTTTTATGAAGAGGAAGAAAATGGTGGAGTTTGGGAAGGATTTGGGGACTTTTCCCCCACGGATGTTC ACAGACAGTTTGCCATTGTCTTCAAAACACCGAAGTATAAGGATGTCAACATTACAAAGCCAGCTTCCGT GTTTGTTCAGCTTCGGAGAAAATCGGATTTGGAAACTAGTGAACCGAAACCCTTTCTCTACTACCCCGAA ATCAAAGACAAGGAGGAAGTACAGAGGAAACGCCAGAAGCTCATGCCCAACTTCTCCGACAGTTTCGGCG GCGGCAGTGGAGCGGGAGCGGGTGGCGGAGGCATGTTCGGTAGTGGCGGTGGCGGTGGAGGGAGTACCGG AAGTCCTGGCCCAGGGTATGGCTTCCCGCACTATGGATTTCCTGCTTACGGTGGGATTGCATTCCATCCC GGAGCCACTAAATCCAACACAGGCATCACCCATGGCACCATAAACACCAAATTTAAAAACGAGCCTAGAG ATTGTGCCAAGAGTGACGACAGGGAGATTCTGAATCCCCCTGAGAAAGAAACACAAGGTGAGGGGCCCAG CCTGTTCATGGCCAGCACCAAGACCGAAGCAATTGCCCCGGCATCCACCATGGAAGACAAGGAAGAGGAT GTGGGGTTTCAGGATAACCTCTTTCTTGAGAAGGCGCTGCAGCTCGCCAAGCGGCATGCCAACGCCCTCT TCGACTACGCGGTTACGGGAGATGTGAAGATGCTGCTGGCGGTACAGCGCCATCTCACCGCGGTGCAGGA TGAGAACGGGGACAGTGTCCTACACTTAGCCATCATCCACCTTCATGCTCAGCTCGTGAGGGATCTGCTA GAAGTGACATCTGGTTCGATCTCTGATGACATCATCAACATGAGAAACGATCTGTATCAGACACCTTTGC ACTTGGCCGTGATCACCAAGCAGGAAGATGTGGTGGAGGACTTGCTGAGGGTTGGGGCTGACCTGAGTCT TCTGGACCGCTGGGGCAACTCTGTCCTGCACCTAGCTGCCAAAGAAGGACATGATAAAATCCTCGGGGTC CTACTCAAGAACAGCAAGGCAGCACTCCTTATCAACCACCCCAACGGGGAAGGTCTGAATGCCATTCACA TAGCTGTGATGAGCAACAGCCTGTCATGTCTGCAGCTGCTTGTGGCTGCTGGGGCAGAAGTCAACGCTCA GGAGCAGAAGTCTGGGCGCACGGCACTGCACCTGGCCGTGGAGTACGACAACATCTCGTTGGCCGGCTGC CTGCTCCTGGAGGGTGACGCCCTCGTGGACAGCACCACCTACGATGGGACGACACCTCTACACATAGCAG CTGGAAGAGGGTCCACCAGGCTGGCAGCTCTTCTCAAAGCAGCAGGAGCAGACCCCCTGGTGGAGAACTT TGAGCCTCTCTATGACCTGGACGACTCTTGGGAGAAGGCTGGAGAAGATGAGGGAGTAGTGCCAGGCACC ACACCCCTGGACATGGCTGCCAACTGGCAGGTATTTGACATACTAAATGGGAAACCATATGAGCCAGTGT TCACATCTGATGACATACTCCCACAAGGGGACATTAAGCAGCTGACAGAAGACACGAGGCTGCAACTCTG CAAGCTCTTGGAAATTCCTGATCCCGACAAGAACTGGGCCACTCTGGCGCAGAAGTTAGGTCTGGGGATA TTGAACAATGCCTTCCGGCTGAGTCCCGCCCCTTCTAAAACTCTTATGGACAACTATGAGGTCTCTGGGG GGACCATCAAAGAGCTGGTGGAGGCCCTGCGACAGATGGGCTACACAGAGGCCATTGAAGTGATCCAGGC AGCCTTCCGCACCCCGGAAACCACAGCCTCCAGCCCCGTGACCACTGCTCAGGCCCACTTGCTGCCTCTC TCGTCCTCCTCCACAAGACAGCACATAGATGAGCTCCGAGATAATGACAGCGTGTGTGACAGTGGTGTGG AGACATCCTTCCGCAAACTCAGCTTCTCGGAGTCCCTCACTGGAGATGGCCCACTGCTATCTCTGAACAA AATGCCTCACAATTATGGGCAGGATGGACCTATAGAAGGCAAAATT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217379 representing NM_001276711 Red=Cloning site Green=Tags(s) MADDDPFGTGQMFHLNTALTHSIFNAELYSTDIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGA SSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGIL HVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAALQQTKEMDLSVV RLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQI RFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPE IKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGSTGSPGPGYGFPHYGFPAYGGIAFHP GATKSNTGITHGTINTKFKNEPRDCAKSDDREILNPPEKETQGEGPSLFMASTKTEAIAPASTMEDKEED VGFQDNLFLEKALQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLL EVTSGSISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDKILGV LLKNSKAALLINHPNGEGLNAIHIAVMSNSLSCLQLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGC LLLEGDALVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGT TPLDMAANWQVFDILNGKPYEPVFTSDDILPQGDIKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGI LNNAFRLSPAPSKTLMDNYEVSGGTIKELVEALRQMGYTEAIEVIQAAFRTPETTASSPVTTAQAHLLPL SSSSTRQHIDELRDNDSVCDSGVETSFRKLSFSESLTGDGPLLSLNKMPHNYGQDGPIEGKI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276711
ORF Size	2916 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276711.1, NP_001263640.1
RefSeq Size	3956 bp
RefSeq ORF	2919 bp
Locus ID	81736
MW	105.6 kDa
Gene Summary	gene encodes a 105 kD protein that can be processed to create a 50 kD protein; the larger protein is a Rel protein-specific transcription inhibitor and the smaller protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex [RGD, Feb 2006]

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