Smarca1 (NM_001271190) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1561046
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217445 representing NM_001271190 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGACACGGCCACAGAGGCAGCCACCGTGGCAGCCTCGGATGCCAGAGCCACAATCGTGGTCC TAGAGGATGAGCAGCCCGGGCCGTCCACCTTTAAAGAGGAGGGAGCGGCTGCTGCCGCCACCGAAGCCAC CACGGCCACTGAGAAGGGCGAGAAGAAGGAGAAAATCACTTCTCCATTTCAACTCAAACTTGCTGCTAAA ACTTCCAAATCTGAAAAGGAAATGGACCCAGAATATGAAGAGAAAATGGTAAATATACCCTTGAAAGCAG ACAGAGCGAAGAGATTTGAGTTTTTGCTGAAGCAGACGGAACTTTTTGCACATTTCATTCAACCTTCAGC ACAGAAGTCTCCAACATCTCCTCTTAACATGAAACTGGCTCGTCCTCGAGTAAAGAAAGATGACAAGCAG AGCTTGATTTCTGTGGGAGACTATCGCCACAGGCGCACAGAACAAGAGGAAGATGAGGAGCTTCTGTCAG AGAGTAGGAAAACATCGAATGTGTGTGTTAGATTTGAGGTCTCACCTTCTTATGTGAAGGGAGGACCCCT GAGAGATTATCAGATTCGAGGACTAAACTGGTTGATCTCTTTGTATGAAAATGGAGTCAATGGTATTTTA GCTGATGAAATGGGCCTTGGGAAGACTTTACAAACCATTGCTTTGCTTGGTTACCTGAAACATTACAGAA ACTTTCCTGGACCCCATATGGTTTTAGTTCCAAAGTCTACTTTACATAACTGGATGAATGAATTTAAACG ATGGGTTCCGTCTCTCCGTGTTATTTGTTTCGTTGGAGACAAAGATGTGAGAGCAGCTTTTATTCGTGAT GAAATGATGCCAGGAGAATGGGATGTTTGTGTGACTTCTTATGAGATGGTAATTAAAGAAAAATCCGTGT TTAAAAAGTTTCACTGGAGATACTTAGTCATTGATGAAGCTCACAGAATAAAAAATGAAAAATCTAAGCT TTCAGAGATTGTTCGTGAGTTCAAGTCAACTAATCGCTTACTTCTAACTGGGACACCTTTGCAGAATAAC TTGCATGAGCTCTGGGCATTACTTAACTTTTTATTGCCGGATGTCTTTAATTCTGCAGATGACTTTGATT CTTGGTTTGATACTAAAAATTGCCTTGGTGATCAAAAGCTTGTGGAAAGACTCCATGCAGTTTTAAAACC ATTCTTGTTACGCCGTATAAAAACTGATGTAGAGAAAAGTCTGCCTCCAAAGAAGGAAATAAAGATTTAC TTGGGACTGAGTAAAATGCAACGAGAATGGTATACAAAAATTCTGATGAAAGATATCGATGTTTTAAACT CTTCTGGCAAGATGGACAAGATGCGACTATTGAATATTCTGATGCAGCTCCGAAAGTGTTGTAATCATCC TTATCTGTTTGATGGAGCAGAGCCTGGTCCACCTTATACGACAGATGAGCACATTGTCAGCAATAGTGGT AAAATGGTAGCTCTGGATAAGCTATTGGCGAGAATTAAAGAACAGGGATCAAGAGTTCTCATCTTTAGCC AGATGACTCGACTGCTAGACATTTTGGAGGATTATTGTATGTGGCGTGGTTATGAGTATTGTCGACTGGA TGGACAAACCCCACATGAAGAAAGAGAGGAAGCAATAGAAGCCTTCAATGCTCCGAATAGCAGCAAATTC ATCTTTATGCTGAGTACTAGGGCTGGAGGACTCGGAATTAACTTGGCAAGTGCTGACGTGGTTATCCTAT ATGATTCAGACTGGAATCCTCAGGTTGATTTACAAGCTATGGATCGGGCACATCGCATTGGTCAGAAGAA GCCAGTACGAGTCTTCCGTCTCATTACAGACAACACAGTGGAAGAAAGGATTGTAGAGAGAGCTGAGATA AAACTGAGACTTGATTCAATTGTTATACAGCAAGGGAGACTCATTGACCAACAATCCAACAAGCTGGCAA AAGAGGAAATGCTACAAATGATACGCCATGGAGCCACACACGTTTTTGCCTGTAAAGAAAGTGAGCTGAC GGATGAAGACATTGTAACTATTCTGGAAAGAGGGGAAAAGAAGACTGCAGAGATGAATGAACGCATGCAG AAAATGGGAGAGTCATCTCTAAGAAATTTCAGAATGGACCTTGAACAAAGTTTGTACAAGTTTGAAGGAG AAGATTATAGAGAAAAACAGAAGCTTGGTACGGTGGAATGGATTGAACCTCCTAAACGAGAACGCAAAGC CAACTACGCAGTGGATGCCTACTTTAGAGAGGCTTTACGTGTCAGCGAGCCAAAGGTCCCAAAGGCTCCT CGGCCTCCAAAACAGCCAAATGTTCAAGATTTTCAATTTTTTCCACCACGCTTATTTGAGCTCCTGGAAA AGGAAATTCTTTATTATCGGAAGACTATAGGTTACAAGGTCCCAAGGAATCCTGAGCTCCCCAACCCAGC TATAGCTCAAAGAGAAGAGCAAAAAAAGATTGATGGAGCAGAACCTCTTACACCACAAGAGAGTGAAGAG AAAGATAAACTTCTCACACAAGGTTTCACAAACTGGACTAAGAGAGATTTTAACCAGTTTATTAAGGCTA ATGAGAAGTATGGTAGAGATGACATTGACAATGTAGCTCGAGAGGTAGAGGGCAAGTCCCCTGAGGAGGT CATGGAGTATTCAGCTGTATTTTGGGAACGCTGCAATGAATTACAGGACATTGAGAAAATTATGGCTCAA ATTGAACGTGGAGAAGCAAGAATTCAGCGAAGAATCAGTATTAAGAAAGCACTGGATGCCAAAATTGCAA GATACAAGGCTCCATTTCATCAGTTACGGATTCAGTACGGAACCAGTAAAGGAAAGAACTATACTGAAGA AGAAGACAGATTCCTCATCTGTATGTTGCACAAAATGGGCTTTGACAGAGAAAATGTATATGAAGAATTG AGACAGTGCGTACGGAATGCTCCCCAGTTTAGATTTGACTGGTTTATCAAGTCGAGAACTGCCATGGAAT TTCAGAGACGCTGTAATACTTTGATTTCATTGATTGAAAAAGAAAATATGGAAATTGAGGAAAGAGAGAG AGCAGAAAAGAAGAAACGGGCAACTAAAACTCCAATGTCACAGAAAAGAAAAGCAGAGTCAGCTACTGAG AGCTCTGGAAAGAAGGATGTCAAGAAGGTGAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217445 representing NM_001271190 Red=Cloning site Green=Tags(s) MEPDTATEAATVAASDARATIVVLEDEQPGPSTFKEEGAAAAATEATTATEKGEKKEKITSPFQLKLAAK TSKSEKEMDPEYEEKMVNIPLKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLARPRVKKDDKQ SLISVGDYRHRRTEQEEDEELLSESRKTSNVCVRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGIL ADEMGLGKTLQTIALLGYLKHYRNFPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDVRAAFIRD EMMPGEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNN LHELWALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIY LGLSKMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVSNSG KMVALDKLLARIKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYCRLDGQTPHEEREEAIEAFNAPNSSKF IFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEI KLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFACKESELTDEDIVTILERGEKKTAEMNERMQ KMGESSLRNFRMDLEQSLYKFEGEDYREKQKLGTVEWIEPPKRERKANYAVDAYFREALRVSEPKVPKAP RPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPELPNPAIAQREEQKKIDGAEPLTPQESEE KDKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNVAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQ IERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEEL RQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMSQKRKAESATE SSGKKDVKKVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271190
ORF Size	3186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271190.1, NP_001258119.1
RefSeq Size	3748 bp
RefSeq ORF	3189 bp
Locus ID	317575
MW	123.5 kDa

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