Pik3ca (NM_133399) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217450 representing NM_133399 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCACGACCATCTTCGGGTGAACTGTGGGGCATCCACTTGATGCCCCCACGAATCCTAGTGGAAT GTTTACTCCCAAATGGAATGATAGTGACTTTAGAATGCCTCCGTGAGGCCACACTAGTCACCATCAAGCA TGAACTGTTCAAAGAGGCCAGGAAATACCCTCTCCATCAGCTTCTGCAAGATGAATCATCTTACATTTTC GTAAGTGTTACCCAAGAAGCAGAAAGGGAAGAATTTTTCGATGAAACAAGACGGCTTTGTGACCTTCGGC TTTTTCAACCCTTTTTAAAAGTAATTGAGCCAGTAGGCAACCGTGAAGAAAAGATCCTCAACCGAGAAAT TGGTTTTGTTATTGGCATGCCAGTGTGTGAATTTGATATGGTTAAAGATCCAGAAGTCCAAGACTTCCGA AGGAACATTCTGAATGTTTGCAAAGAAGCCGTGGACCTGCGGGATCTCAACTCGCCTCATAGCAGAGCAA TGTATGTCTACCCTCCAAATGTCGAGTCTTCCCCAGAACTGCCAAAGCACATCTACAACAAGTTAGATAA AGGACAAATCATAGTGGTGATTTGGGTGATAGTCTCTCCAAACAACGACAAGCAGAAGTACACTCTGAAG ATCAACCATGACTGCGTGCCAGAGCAAGTCATTGCTGAGGCCATCAGGAAGAAGACCCGGAGCATGTTGC TGTCCTCGGAGCAGCTGAAACTCTGTGTCTTAGAATACCAGGGCAAGTACATTCTCAAAGTGTGTGGCTG TGATGAGTACTTCCTAGAGAAGTACCCTCTGAGTCAGTACAAGTACATAAGAAGCTGTATAATGCTGGGG AGGATGCCCAACTTGATGCTGATGGCCAAGGAGAGCCTGTACTCTCAGCTGCCGATCGATAGCTTCACAA TGCCATCCTACTCCAGGCGCATTTCCACAGCGACACCCTATATGAACGGGGAGACTGCTACGAAATCCCT CTGGGTTATAAATAGCGCGCTCAGAATAAAAATTCTGTGTGCAACCTATGTAAATGTAAATATTCGAGAC ATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACCCTTATGTGACAATGTGAATACTC AAAGAGTCCCTTGTTCCAATCCTAGGTGGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCC TCGTGCTGCCCGCCTTTGCCTTTCAATCTGCTCTGTTAAAGGCCGAAAGGGTGCTAAGGAGGAGCACTGT CCGTTGGCCTGGGGAAACATAAACTTGTTTGATTATACAGACACCCTAGTGTCCGGGAAAATGGCTTTGA ATCTCTGGCCTGTACCACATGGGTTGGAAGATCTGCTGAACCCTATTGGTGTTACTGGGTCAAATCCAAA TAAAGAAACTCCATGCTTAGAGTTGGAGTTTGATTGGTTCAGCAGTGTGGTGAAGTTTCCAGATATGTCT GTGATCGAAGAGCATGCCAATTGGTCTGTGTCCCGAGAAGCCGGATTCAGTTACTCTCATACAGGACTGA GTAACAGACTAGCCAGAGACAATGAGTTAAGAGAAAATGACAAGGAACAGCTCCGAGCACTTTGTACCCG GGACCCACTGTCTGAAATCACTGAACAAGAGAAAGACTTCCTATGGAGCCACAGACACTACTGTGTAACT ATTCCTGAAATCCTACCCAAATTGCTTCTGTCTGTCAAGTGGAATTCCAGAGATGAAGTGGCCCAGATGT ACTGCTTAGTAAAAGATTGGCCTCCAATCAAACCAGAGCAAGCCATGGAGCTCCTGGACTGTAACTACCC AGACCCCATGGTTCGGAGCTTTGCTGTCCGGTGCTTGGAAAAATACTTAACAGATGACAAACTTTCTCAG TACCTCATCCAGCTTGTACAGGTCTTAAAATATGAACAGTATTTGGATAACCTGCTTGTGAGATTTTTAC TCAAGAAAGCACTGACAAATCAAAGGATTGGCCATTTTTTCTTTTGGCATTTAAAATCTGAGATGCACAA TAAGACTGTCAGTCAGAGGTTCGGCCTGCTGTTGGAGTCCTACTGCCGTGCCTGTGGGATGTATCTGAAG CACCTGAACAGACAGGTAGAGGCCATGGAGAAGCTCATCAATCTAACTGACATCCTCAAGCAGGAGAAGA AGGATGAGACACAGAAGGTACAGATGAAGTTCTTGGTTGAACAGATGAGACAGCCAGATTTCATGGATGC TTTGCAGGGTTTTCTGTCCCCTCTAAATCCTGCTCATCAACTAGGAAACCTCAGGCTTGAAGAGTGTCGA ATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATTGGGAGAACCCAGACATCATGTCAGAGCTACTGT TTCAGAACAATGAGATCATCTTTAAAAATGGCGATGACTTACGGCAAGACATGTTAACCCTTCAGATCAT CCGAATCATGGAGAACATCTGGCAAAACCAAGGCCTTGACCTTCGCATGCTACCTTATGGCTGTCTATCC ATTGGGGACTGTGTGGGTCTCATCGAGGTGGTGAGAAACTCTCACACCATCATGCAGATTCAGTGCAAAG GAGGCCTGAAAGGGGCACTGCAGTTCAACAGCCACACGCTGCATCAGTGGCTCAAGGACAAGAACAAGGG CGAGATATATGACGCAGCCATTGACCTGTTCACTCGGTCCTGCGCTGGGTACTGCGTGGCAACCTTTATC TTGGGAATTGGAGACCGGCACAACAGCAACATCATGGTGAAAGATGACGGACAGCTGTTTCATATAGATT TTGGGCACTTTTTGGATCACAAGAAGAAAAAATTTGGCTATAAACGGGAACGTGTGCCGTTTGTTTTGAC GCAGGATTTCTTAATAGTGATTAGTAAAGGAGCACAAGAGTACACAAAGACCAGAGAGTTTGAGAGGTTT CAGGAGATGTGTTACAAGGCGTACCTAGCAATTCGGCAGCATGCCAATCTCTTCATCAACCTTTTCTCCA TGATGCTTGGCTCCGGAATGCCAGAACTGCAGTCTTTCGATGATATTGCATATATTCGAAAGACTCTAGC CTTAGACAAAACTGAGCAAGAGGCTCTGGAGTATTTCACAAAGCAAATGAATGACGCACATCATGGTGGC TGGACAACAAAAATGGACTGGATCTTCCACACCATCAAGCAGCATGCATTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217450 representing NM_133399 Red=Cloning site Green=Tags(s) MPPRPSSGELWGIHLMPPRILVECLLPNGMIVTLECLREATLVTIKHELFKEARKYPLHQLLQDESSYIF VSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFVIGMPVCEFDMVKDPEVQDFR RNILNVCKEAVDLRDLNSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQKYTLK INHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCGCDEYFLEKYPLSQYKYIRSCIMLG RMPNLMLMAKESLYSQLPIDSFTMPSYSRRISTATPYMNGETATKSLWVINSALRIKILCATYVNVNIRD IDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHC PLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMS VIEEHANWSVSREAGFSYSHTGLSNRLARDNELRENDKEQLRALCTRDPLSEITEQEKDFLWSHRHYCVT IPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNYPDPMVRSFAVRCLEKYLTDDKLSQ YLIQLVQVLKYEQYLDNLLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLK HLNRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRQPDFMDALQGFLSPLNPAHQLGNLRLEECR IMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLS IGDCVGLIEVVRNSHTIMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCVATFI LGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGAQEYTKTREFERF QEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALEYFTKQMNDAHHGG WTTKMDWIFHTIKQHALN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133399
ORF Size	3204 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133399.2, NP_596890.2
RefSeq Size	6435 bp
RefSeq ORF	3207 bp
Locus ID	170911
UniProt ID	A0A0G2K344
MW	124.4 kDa
Gene Summary	expression of the human homolog is associated with increased cell proliferation and decreased apoptosis and may play a role in ovarian cancer progression [RGD, Feb 2006]

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