Hdac4 (NM_053449) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217458 representing NM_053449 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCCAAAGCCATCCAGATGGACTTTCTGGCCGAGACCAGCCTGTGGAGCTGCTGAATCCTGCCC GCGTGAACCACATGCCCAGCACGGTGGACGTGGCTACAGCGCTGCCTCTGCAGGTGGCCCCTGCAGCAGT ACCCATGGACCTGCGCTTGGACCACCAGTTCTCACTGCCCTTGGAACCTGCACTGCGGGAGCAGCAACTG CAGCAGGAACTCCTAGCACTGAAGCAGAAGCAGCAGATCCAGCGGCAGATCCTCATTGCAGAGTTCCAGC GTCAGCATGAGCAGTTGTCCCGACAGCATGAGGCACAGCTGCATGAACATATCAAGCAGCAGCAGGAGAT GCTGGCCATGAAGCACCAGCAGGAGCTGCTGGAGCACCAGCGGAAACTGGAGCGGCACCGGCAAGAGCAG GAGCTGGAGAAGCAGCACCGTGAGCAGAAGCTGCAGCAGCTCAAGAACAAGGAGAAGGGCAAAGAGAGTG CTGTGGCGAGCACAGAGGTGAAGATGAAGCTGCAGGAGTTTGTTCTCAACAAGAAGAAGGCTCTGGCCCA CCGGAACCTGAACCACTGCATGTCCAGCGACCCCCGCTACTGGTATGGGAAGACACAGCACAGCTCCCTT GACCAGAGCTCTCCACCCCAGAGTGGGGTGTCAGCCTCCTACAACCACCCTGTCTTGGGAATGTACGACG CCAAAGATGACTTCCCTCTTAGGAAAACAGCTTCTGAACCTAACCTGAAATTACGGTCAAGGCTTAAGCA GAAAGTAGCTGAGAGACGGAGCAGCCCCCTGCTGCGCAGGAAAGATGGCCCTGTGGCCACTGCTCTAAAA AAGCGACCCCTGGATGTTACAGACTCCGCATGTAGCAGCGCCCCTGGCTCCGGTCCCAGCTCCCCCAATA GCAGCTCTGGCAACGTCAGCACCGAGAATGGCATTGCACCCACCGTGCCCAGCACTCCAGCTGAGACGAG CTTGGCACACAGACTTGTGACTCGAGAAGGCTCTGTTGCCCCACTTCCTCTCTATACATCACCATCCTTG CCCAACATCACCCTGGGACTTCCTGCCACTGGCCCTGCTGCTGGTGCAGCAGGCCAGCAGGATGCTGAGA GGCTTGCTCTCCCAGCCCTCCAGCAGCGAATCTCCTTGTTCCCTGGGACCCACCTCACCCCATACCTGAG CACCTCGCCCCTGGAGCGGGACGGTGGAGCAGCTCACAACCCCCTCCTGCAGCACATGGTCCTGCTGGAG CAGCCACCCACCCAGACGCCCCTTGTCACAGGCCTGGGGGCACTGCCCCTCCACACACAGTCCCTGGTTG GTGCGGACAGGGTGTCCCCATCCATTCACAAGCTGCGGCAGCACCGACCTCTGGGGCGCACGCAGTCAGC ACCCCTGCCGCAGAACGCACAGGCCCTGCAGCACCTGGTGATCCAGCAGCAGCACCAGCAGTTTCTGGAG AAGCACAAGCAACAGTTCCAGCAGCAGCAGCTGCACCTCAGCAAGATGATTTCTAAACCCAGTGAGCCAC CTCGGCAGCCTGAGAGCCACCCGGAGGAGACAGAGGAGGAGCTCCGTGAACACCAGGCCTTGCTGGATGA GCCCTACCTAGATCGGCTCCCTGGGCAGAAGGAGCCCTCCTTAGCTGGTGTGCAGGTGAAGCAGGAGCCC ATCGAGAGTGAGGAGGAAGAAGTAGAGGCCACTCGAGAGGCGGAACCCAGCCAGCGCCCAGCCACTGAAC AGGAGCTTCTCTTTAGACAGCAAGCTCTTCTGCTGGAGCAGCAGAGGATCCACCAGTTAAGGAACTACCA GGCATCTATGGAGGCTGCTGGCATCCCTGTGTCATTTGGCAGCCACAGACCTCTGTCTCGCGCACAGTCC TCCCCAGCATCTGCCACCTTCCCCATGTCAGTCCAGGAGCCCCCCACCAAACCAAGGTTCACCACAGGCC TTGTGTATGACACACTCATGTTGAAGCACCAGTGCACCTGTGGGAACACCAACAGCCACCCGGAGCATGC TGGGAGGATCCAGAGCATCTGGTCCCGCCTGCAGGAGACTGGCCTCCGTGGCAAGTGTGAGTGCATCCGC GGACGCAAGGCCACACTGGAAGAGCTGCAGACAGTGCACTCGGAGGCCCACACACTCCTCTATGGCACAA ACCCTCTCAACAGACAGAAACTGGACAGCTCGCTGACCTCCGTGTTTGTCAGGCTTCCTTGTGGTGGTGT TGGGGTGGATAGTGATACCATATGGAATGAGGTGCACTCGTCTGGGGCAGCCCGCCTGGCTGTAGGCTGT GTAGTGGAGCTGGTCTTCAAGGTGGCCACGGGAGAGCTAAAGAATGGCTTTGCTGTGGTTCGTCCCCCAG GACACCATGCCGAGGAGAGTACACCCATGGGTTTCTGCTACTTTAATTCTGTGGCAATTGCAGCCAAACT TCTCCAGCAGAGGTTGAATGTGAGCAAGATCCTCATTGTAGACTGGGATGTGCACCATGGGAATGGGACC CAGCAGGCCTTCTACAATGACCCCAATGTTCTCTACATGTCCCTGCACCGCTATGACGATGGGAACTTCT TCCCAGGAAGTGGAGCACCAGATGAGGTGGGCACAGGGCCAGGCGTGGGTTTCAATGTCAACATGGCCTT CACGGGTGGCCTCGATCCCCCCATGGGAGACGCCGAGTACCTGGCAGCCTTCAGAACGGTGGTCATGCCA ATCGCAAATGAGTTTGCCCCAGATGTGGTACTGGTGTCCTCGGGCTTCGATGCTGTGGAGGGCCACCCCA CACCTCTTGGAGGGTACAATCTCTCTGCCAAATGTTTCGGGTACTTGACAAAACAGCTGATGGGCTTAGC TGGTGGCCGGATCGTGCTGGCCCTGGAGGGAGGCCATGACCTGACAGCCATCTGTGATGCTTCTGAAGCA TGTGTTTCTGCTCTGCTGGGAAATGAGCTTGAGCCTCTGCCAGAAAAGGTTCTACATCAGAGACCCAATG CCAATGCCGTCCACTCCATGGAGAAAGTGATGGGCATCCACAGTGAGTACTGGCGCTGCCTGCAGCGTTT GTCCCCCACTGTGGGGCACTCTCTGATTGAGGCGCAGAAGTGTGAGAATGAAGAAGCTGAGACAGTCACC GCCATGGCCTCGCTGTCTGTAGGCGTCAAACCTGCTGAGAAGAGATCTGAGGAGGAGCCCATGGAGGAGG AGCCACCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217458 representing NM_053449 Red=Cloning site Green=Tags(s) MSSQSHPDGLSGRDQPVELLNPARVNHMPSTVDVATALPLQVAPAAVPMDLRLDHQFSLPLEPALREQQL QQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLHEHIKQQQEMLAMKHQQELLEHQRKLERHRQEQ ELEKQHREQKLQQLKNKEKGKESAVASTEVKMKLQEFVLNKKKALAHRNLNHCMSSDPRYWYGKTQHSSL DQSSPPQSGVSASYNHPVLGMYDAKDDFPLRKTASEPNLKLRSRLKQKVAERRSSPLLRRKDGPVATALK KRPLDVTDSACSSAPGSGPSSPNSSSGNVSTENGIAPTVPSTPAETSLAHRLVTREGSVAPLPLYTSPSL PNITLGLPATGPAAGAAGQQDAERLALPALQQRISLFPGTHLTPYLSTSPLERDGGAAHNPLLQHMVLLE QPPTQTPLVTGLGALPLHTQSLVGADRVSPSIHKLRQHRPLGRTQSAPLPQNAQALQHLVIQQQHQQFLE KHKQQFQQQQLHLSKMISKPSEPPRQPESHPEETEEELREHQALLDEPYLDRLPGQKEPSLAGVQVKQEP IESEEEEVEATREAEPSQRPATEQELLFRQQALLLEQQRIHQLRNYQASMEAAGIPVSFGSHRPLSRAQS SPASATFPMSVQEPPTKPRFTTGLVYDTLMLKHQCTCGNTNSHPEHAGRIQSIWSRLQETGLRGKCECIR GRKATLEELQTVHSEAHTLLYGTNPLNRQKLDSSLTSVFVRLPCGGVGVDSDTIWNEVHSSGAARLAVGC VVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAIAAKLLQQRLNVSKILIVDWDVHHGNGT QQAFYNDPNVLYMSLHRYDDGNFFPGSGAPDEVGTGPGVGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMP IANEFAPDVVLVSSGFDAVEGHPTPLGGYNLSAKCFGYLTKQLMGLAGGRIVLALEGGHDLTAICDASEA CVSALLGNELEPLPEKVLHQRPNANAVHSMEKVMGIHSEYWRCLQRLSPTVGHSLIEAQKCENEEAETVT AMASLSVGVKPAEKRSEEEPMEEEPPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053449
ORF Size	3231 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053449.1, NP_445901.1
RefSeq Size	4015 bp
RefSeq ORF	3234 bp
Locus ID	363287
UniProt ID	Q99P99
MW	118.7 kDa
Gene Summary	human homolog is a component of the DNA damage response pathway and is also involved in repression of muscle differentiation [RGD, Feb 2006]

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