Anks1b (NM_001271371) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AIDA-1; EB-1; RGD1565556
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217566 representing NM_001271371 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGACCAGGAGCTGCTAGAGGCTGCTCGTACTGGCAATGTGGCTCTGGTCGAGAAACTCCTAT CTGGCAGGAAAGGAGGGATCCTGGGTGGTGGATCCGGACCTCTGCCCCTGTCTAATCTGCTAAGCATCTG GCGAGGTCCCAACGTGAACTGCACAGACAGTTCGGGTTACACCGCTTTGCACCACGCAGCCTTAAATGGA CATAAGGACATAGTTCTCAAACTACTTCAATTCGAGGCGTCCACCAATGTCGCAGACAACAAAGGTTACT TCCCCATTCACCTGGCTGCCTGGAAAGGAGACGTGGAGATTGTGAAGATTCTTATCCATCACGGGCCTTC ACATTCCAGAGTCAATGAACAGAACAACGAGAACGAAACTGCCCTCCACTGTGCAGCGCAATACGGACAC TCAGAAGTAGTGGCTGTTCTCCTAGAGGAGCTCACCGATCCCACCATTAGGAACAGCAAGCTCGAAACAC CGCTGGACTTGGCAGCGCTCTACGGAAGGCTGAGGGTGGTGAAGATGATCATCAGCGCGCACCCAAACTT AATGAGCTGTAACACGCGGAAGCACACGCCGCTCCACCTCGCGGCCCGCAATGGTCACAAGGCCGTGGTG CAGGTGCTGCTGGAGGCGGGGATGGACGTGAGCTGTCAAACAGAGAAGGGGAGTGCTCTTCACGAAGCGG CGTTGTTTGGGAAGGTGGATGTGGTGCGAGTTCTGTTAGAAACAGGAATTGATGCCAACATAAAGGACAG CCTAGGTAGAACCGTCTTAGACATTCTGAAAGAGCACCCGTCTCAGAAGTCTCTCCAGATTGCAACGCTC CTGCAAGACTATTTGGAAGGCGTGGGAAGGTCGGTGGTCCTCGAGGAAGAGCATGCTCAGGAAGACACAG CACAAGAGACACGCCTTTCGTCTCCTGCCCAGTCTCCTTCTCAAAAGACTAAAAGTGAAACTGTCACTGG AGAACTATCGAAACTCCTGGATGAAATAAAACTCTGTCAAGAAAAGGATTATTCCTTTGAAGATCTGTGC CATACAATCTCAGACCACTACTTAGATAATTTGAGCAAGATCTCAGAGGAAGAACTTGGGAAAAATGGAA GCCAGAGTGTAAGAACTTCATCTACAATAAATTTGTCACCAGGAGAAGTGGAAGACGAAGAGGAGGATCC AAACTCTTGTGGACCCACGGGACTTTGGGAAGCGCTAACTCCTTGTAACGGATGTAGGAACCTTGGCTTT CCCATGCTCGCACAGGAGTCTTATCCAAAGAAGAGGAATTACCCCATGGAAATTGTACCATCTGCTTCTC TGGACACATTCCCTTCAGAAAATGAGAACTTTCTTTGTGAACTCGTGGACACAGCTGTTACAAAGAAGCC CTGTTCCTTAGAAATCGCAAGGGCACCTTCTCCGAGAACTGATAATGCCTCTGAGGTAGCAATTACTGCT CCAGGAACTGGTCACCATAGAAACAGCTCTACAGGCCCAACACCTGACTGCTCACCTCCATCCCCCGACA CTGCCCTAAAAAATATCGTAAAAGTTATCCGACCTCAGCCAAAACAACGAACATCTATTGTGTCGTCTCT GGATTTCCAGCGAATGAATCATAACCAAGAATACTTTGAAATCAGCACATCTACAGGCTGTACCAGCTTT ACTTCGAGCCCTCCAGTGAGCCCACCCACCTCCTCTGTGGAAACCACAGAAATCAAGAACGAGGGAGCTG AACACACAGATGACCTCTCTCAGCAGGAAGATGACGAACCCCCAAAAGAGTATGATGCTGGGCAATTCGC AGGCCTTCTCCACGGGTCCTCTCCAGCCTGTGAGGCTCCAGAAAATCCGTTTCACCTGTATGGGAAAAGA AATCAAGGTGAAGACGGACAAGAGGAAGCCAGCTTGGCCAACAGTCCTTTGCCTTTCAAACAGACTCCAA TAGAAAATAACCCAGAGCCTTCAGTGAAGAAAATTAAACCCAAAGTGGTCAGTAGAACGATTTTCCACAA AAGAAGCCACCAACTTGAGAACCATACCATCGTTGGCACAAGAATGTCCAGGGGTGGATCCCGCAACGGC GACCAATGGGGTGTGAATCCAGGGGGCTTCGTGGAGAGAGCGTGCACTCTAGGGAGAATAAGGTCATTGC CTAAAGCCCTGATCGACATGCATTTATCCAAAAATGTCTCCAAGTCCGATTCCGATCTTATTGCGTACCC TTCCAAGGACAAAGCGAGAGTTAACTGGAGCAAATCGTCAACTGCAGAGCGCAGTTCCAAAGATAATTCT GAAAGAACGCCTTCCTTCACATCTGAATGGGAAGAAATCGACAAAATAATGAACTCGATTGACGTTGGAA TCAACAGCGAACTTGAAGGGATGAACGGACCCAGATGCCCTGTCCAAACAGTAGGACAATGGTTGGAAAG CATTGGGCTACCTCAGTACGAGAACCACCTGACGGCTAATGGATTTGACAATGTACAGTTTATGGGAAGC AATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAACTCTGGTCACAGACAAAGAATTCTAC AGGCGATCCAGCTGCTTCCAAAGATGAGGCCCATCGGCCACGATGGCTACCATCCCACCTCTGTAGCTGA GTGGCTGGATTCCATCGAGCTGGGCGACTACACGAAAGCCTTTCTCATCAATGGCTACACGTCGATGGAC CTGTTGAAAAAGATCTGGGAGCTTGAACTTATTAACGTTTTAAAAATCAGTTTGATTGGCCACAGGAAGC GCATTTTGGCATCTCTGGGAGACAGACTGCACGAGGACCCTCCACAGAAGCCCCCTCGGTCCATCACCCT CAGGGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGCACTTACACCACT GGTGGCTCCCTAGACGTCCCTCACATTATCATGCAGGGCGATGCAAGGAGGAGAAGAAATGAAAACTACT TTGATGATATTCCCCGATCAAAGCTGGAGAGACAGATGGCTCAGACAGGGGACTGGGGAGAACCTTCCAT CACCTTGCGACCTCCTAATGAAGCCACAGCCTCAACTCCAGTCCAGTACTGGCAGCATCACCCAGAAAAG CTCATCTTCCAGTCGTGTGACTACAAAGCCTTTTATTTAGGTTCTATGCTGATAAAAGAGCTGAGGGGAA CGGAATCAACACAGGACGCTTGCGCAAAGATGCGGGCTAACTGTCAGAAGTCTACGGAGCAAATGAAGAA AGTCCCCACTATCATTCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCGGCAAATAAGAACATA ATTGCCGAGCATGAAATTCGTAATATCTCTTGTGCTGCCCAGGACCCAGAAGACCTCTCAACCTTTGCTT ATATCACAAAAGATCTGAAGTCCAACCACCACTACTGTCACGTGTTTACTGCCTTTGATGTGAATTTAGC CTATGAAATCATCCTAACGCTGGGGCAGGCATTTGAGGTCGCTTACCAGCTAGCTCTACAAGCAAGAAAA GGGGGCCATTCCTCCACGCTTCCGGAGAGCTTTGAGAACAAGCCCTCGAAACCCATCCCAAAGCCCCGAG TTAGCATTCGCAAGTCAGTGCAGATCGACCCTTCTGAGCAAAAGACTCTGGCCAATCTGCCGTGGATTGT GGAGCCGGGGCAAGAAGCCAAGAGGGGCATTAATACCAAGTATGAAACCACGATTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217566 representing NM_001271371 Red=Cloning site Green=Tags(s) MGKDQELLEAARTGNVALVEKLLSGRKGGILGGGSGPLPLSNLLSIWRGPNVNCTDSSGYTALHHAALNG HKDIVLKLLQFEASTNVADNKGYFPIHLAAWKGDVEIVKILIHHGPSHSRVNEQNNENETALHCAAQYGH SEVVAVLLEELTDPTIRNSKLETPLDLAALYGRLRVVKMIISAHPNLMSCNTRKHTPLHLAARNGHKAVV QVLLEAGMDVSCQTEKGSALHEAALFGKVDVVRVLLETGIDANIKDSLGRTVLDILKEHPSQKSLQIATL LQDYLEGVGRSVVLEEEHAQEDTAQETRLSSPAQSPSQKTKSETVTGELSKLLDEIKLCQEKDYSFEDLC HTISDHYLDNLSKISEEELGKNGSQSVRTSSTINLSPGEVEDEEEDPNSCGPTGLWEALTPCNGCRNLGF PMLAQESYPKKRNYPMEIVPSASLDTFPSENENFLCELVDTAVTKKPCSLEIARAPSPRTDNASEVAITA PGTGHHRNSSTGPTPDCSPPSPDTALKNIVKVIRPQPKQRTSIVSSLDFQRMNHNQEYFEISTSTGCTSF TSSPPVSPPTSSVETTEIKNEGAEHTDDLSQQEDDEPPKEYDAGQFAGLLHGSSPACEAPENPFHLYGKR NQGEDGQEEASLANSPLPFKQTPIENNPEPSVKKIKPKVVSRTIFHKRSHQLENHTIVGTRMSRGGSRNG DQWGVNPGGFVERACTLGRIRSLPKALIDMHLSKNVSKSDSDLIAYPSKDKARVNWSKSSTAERSSKDNS ERTPSFTSEWEEIDKIMNSIDVGINSELEGMNGPRCPVQTVGQWLESIGLPQYENHLTANGFDNVQFMGS NVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDYTKAFLINGYTSMD LLKKIWELELINVLKISLIGHRKRILASLGDRLHEDPPQKPPRSITLREPSGNHTPPQLSPSLSQSTYTT GGSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQMAQTGDWGEPSITLRPPNEATASTPVQYWQHHPEK LIFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSYKGVKFIDAANKNI IAEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLALQARK GGHSSTLPESFENKPSKPIPKPRVSIRKSVQIDPSEQKTLANLPWIVEPGQEAKRGINTKYETTIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271371
ORF Size	3768 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271371.1, NP_001258300.1
RefSeq Size	5579 bp
RefSeq ORF	3771 bp
Locus ID	314721
MW	138.7 kDa
Gene Summary	Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.[UniProtKB/Swiss-Prot Function]

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