Top2b (NM_001100858) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217667 representing NM_001100858 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGTCCAGCCTCGCGGGCGTTGACGGGGCGCTGACCTGGGTGAACAATGCTGCAAAAAAGGAAG AGTTGGAGACAGCGAACAAAAATGATTCTTCCAAGAAGTTGTCTGTTGAGAGAGTGTACCAGAAGAAGAC CCAACTGGAGCATATTCTCCTTCGTCCTGATACATACATTGGATCAGTGGAACCACTGACACAGTTAATG TGGGTGTATGACGAAGATGTAGGCATGAACTGCAGAGAGGTTACCTTTGTTCCGGGCTTATACAAGATCT TTGATGAAATTTTGGTCAATGCTGCTGACAACAAGCAGAGGGATAAGAACATGACCTGTATTAAGGTTTC TATTGATCCTGAATCTAATATTATAAGCATTTGGAATAATGGAAAAGGCATTCCAGTAGTAGAACACAAA GTAGAGAAAGTTTATGTTCCTGCTTTAATTTTTGGACAGCTTTTAACATCGAGTAATTACGATGATGATG AGAAAAAAGTTACAGGAGGCCGTAATGGTTATGGTGCAAAACTTTGTAATATTTTTAGTACAAAGTTTAC AGTAGAAACGGCTTGCAAAGAATACAAACATAGTTTTAAGCAGACATGGATGAATAATATGATGAAGACT TCTGAAGCCAAAATCAAACATTTTGATGGGGAAGATTATACATGCATAACATTCCAGCCAGATCTAGCCA AGTTTAAGATGGAAAAACTTGACAAGGACATTGTGGCCCTCATGACCAGAAGGGCATACGATTTAGCTGG TTCCTGCAAAGGCGTCAAGGTCATGTTCAATGGCAAGAAGTTGCCTGTAAATGGATTTCGCAGCTATGTA GACCTTTATGTGAAAGACAAATTGGATGAAACTGGGGTCGCTCTCAAAGTTATTCATGAACTTGCAAATG AAAGATGGGATGTTTGTCTAACCTTGAGTGAAAAAGGTTTCCAGCAGATCAGCTTTGTGAATAGTATTGC AACTACAAAAGGTGGACGGCACGTGGATTACGTAGTAGATCAAGTTGTTGGTAAACTGATTGAAGTAGTT AAGAAAAAGAACAAAGCTGGTGTATCAGTGAAGCCATTTCAAGTTAAAAACCACATATGGGTTTTTATTA ATTGTCTTATTGAAAATCCAACCTTTGATTCTCAAACTAAGGAAAACATGACTCTGCAGCCCAAGAGCTT TGGGTCTAAATGCCAGCTGTCAGAAAAGTTCTTTAAAGCGGCCTCTAATTGTGGCATTGTAGAAAGCATA TTGAACTGGGTGAAATTTAAGGCTCAGACTCAGCTGAATAAGAAGTGCTCCTCAGTAAAGTACAGCAAAA TCAAAGGCATTCCCAAACTGGATGATGCCAATGATGCTGGTGGCAAACATTCCCTGGAGTGTACATTAAT ATTAACAGAGGGGGACTCTGCCAAATCTCTAGCTGTCTCTGGATTAGGTGTGATTGGGAGAGACAGATAT GGAGTCTTCCCTCTCAGGGGTAAAATTCTCAATGCACGAGAAGCGTCTCATAAACAGATTATGGAAAATG CAGAAATAAACAATATTATTAAAATAGTTGGTTTGCAATACAAGAAAAGTTATGATGATGCAGAATCTCT GAAAACTTTACGTTATGGGAAGATTATGATTATGACTGATCAGGATCAAGATGGTTCTCACATTAAAGGC CTGCTAATCAATTTTATTCATCACAATTGGCCATCACTTTTGAAGCATGGCTTTCTTGAAGAATTCATTA CTCCCATTGTAAAGGCGAGCAAAAATAAGCAGGAACTTTCCTTTTATAGTATTCCTGAATTTGATGAGTG GGAAAAACATATAGAAAACCAGAAAGCCTGGAAAATAAAGTACTACAAAGGATTGGGTACCAGTACAGCT AAGGAAGCCAAGGAATATTTTGCTGATATGGAAAGGCATCGCATTTTGTTTAGATATGCTGGCCCTGAGG ATGATGCTGCCATTACCTTGGCATTTAGTAAGAAGAAGATTGATGACAGAAAAGAATGGTTAACCAACTT TATGGAGGATCGGAGACAGCGGAGGTTACATGGCCTACCAGAGCAATTTTTATATGGTACAGCAACTAAG CATTTGACATACAATGATTTCATCAACAAGGAATTGATTCTCTTCTCAAACTCGGACAATGAGAGATCTA TACCCTCTCTTGTGGATGGCTTCAAGCCAGGTCAACGAAAAGTTTTGTTTACCTGTTTCAAGAGGAATGA TAAACGTGAAGTTAAAGTCGCACAGCTGGCTGGATCTGTTGCTGAAATGTCTGCTTATCATCATGGAGAA CAAGCGTTGATGATGACTATTGTGAATTTGGCTCAGAATTTTGTGGGCAGCAACAACATTAACTTGCTTC AGCCCATTGGTCAGTTTGGAACTCGGCTTCACGGTGGCAAAGATGCTGCAAGCCCCCGTTATATCTTCAC AATGTTAAGCTCTCTGGCAAGACTACTTTTTCCAGCTGTGGATGATAACTTGCTTAAGTTCCTTTATGAT GATAATCAGCGTGTAGAGCCGGAGTGGTATATCCCCATAATCCCCATGGTTTTGATTAATGGTGCTGAGG GTATTGGGACTGGATGGGCTTGTAAATTGCCCAACTATGATGCCAGAGAAATTGTGAACAATGTCAGGCG GATGCTAGAAGGTTTGGATCCTCACCCCATGCTTCCAAACTATAAGAACTTCAAAGGCACCATCCAGGAG CTTGGTCAGAACCAGTATGCTGTTAGTGGTGAGATATTTGTGGTGGACAGAAACACAGTAGAGATTACAG AACTTCCAGTTAGAACCTGGACACAGGTATACAAGGAGCAGGTCCTAGAACCTATGCTTAATGGAACAGA TAAAACACCAGCACTGATTTCTGATTACAAAGAATATCACACTGATACAACTGTGAAATTTGTGGTGAAG ATGACAGAAGAGAAGCTCGCCCAAGCGGAAGCTGCCGGCCTGCATAAAGTTTTTAAACTCCAAACCACTC TTACTTGCAATTCCATGGTACTCTTTGATCATATGGGATGTCTGAAGAAGTATGAAACTGTGCAAGACAT TTTGAAAGAATTCTTTGATTTACGGTTAAGTTATTATGGTTTACGGAAGGAGTGGCTTGTAGGAATGTTG GGAGCAGAATCCACAAAGCTTAACAATCAAGCCCGTTTCATTTTAGAGAAGATACAAGGGAAAATCACGA TAGAGAATAGGTCAAAGAAAGATTTGATTCAAATGTTAGTCCAGAGAGGTTATGAATCTGACCCAGTGAA AGCCTGGAAAGAAGCACAAGAAAAGGCTGCGGAAGAGGAAGATGCACAAAACCCACATGATGACAGTTCC TCTGACTCAGGAACTCCTTCAGGCCCTGATTTTAATTATATTTTAAATATGTCTCTATGGTCTCTTACTA AAGAAAAAGTTGAAGAACTCATTAAACAGAGAGATACAAAAGGACGAGAGGTTAATGATCTCAAAAGGAA ATCTCCCTCAGATCTTTGGAAAGAAGATTTAGCAGCATTTGTTGAAGAATTGGATAAAGTGGAAGCACAA GAACGAGAAGACATTCTGGCAGGGATGTCTGGGAAAGCAGTGAAAGGCAAAGTCGGCAAAGCTAAAGTAA AGAAGCTCCAGCTGGAGGAGACGATGCCCTCGCCTTACGGGAGAAGAATCGTTCCTGAGATTACTGCCAT GAAGGCAGACGCCAGCAGAAAACTGCTGAAGAAGAAGAAGGGTGATCCTGATACTACAGTTGTGAAAGTA GAATTTGATGAAGAATTTAGTGGAACACCAGCAGAAGGTACAGGAGAAGAGACATTGACACCATCTGCTC CTGTAAATAAAGGCCCCAAACCCAAAAGGGAGAAAAAGGAGCCTGGTACCAGGGTTAGAAAAACACCTGC ATCAACTGGCAAACCTAATACAAAGAAAGTGAAGAAGCGGAATCCTTGGTCAGATGATGAGTCCAAATCA GAGAGTGACTTGGAAGAAGCAGAGCCTGTGGTTATCCCAAGAGATTCTTTGCTTAGGAGAGCAGCTGCTG AAAGACCTAAGTACACTTTTGATTTCTCAGAAGAGGAGGAGGATGATGCTGATGATGATGACGACAATAA TGATTTAGAAGAATTGAAAGTTAAAGCATCTCCCATAACAAATGATGGGGAGGATGAATTTGTTCCTTCA GATGGCATAGATAAAGATGAGTATGCCTTTTCTCCAGGCAAATCCAAAGCTACACCAGAAAAATCTTCAC ATGACAAAAAAAGTCAGGATTTTGGAAATCTTTTTTCATTTCCTTCATATTCTCAGAAGTCAGAAGATGA TTCAGCTAAATTTGACAGTAATGAAGAAGACACCACATCTGTTTTTGCACCATCATTTGGTCTGAAGCAA ACAGATAAAGTTCCAAGTCAAACAGTCGCTGCTAAAAAGGGTAAAGCACCTTCAGATGCAGCAGCCCCTA AGGCCAAGAGAGCCCCTAGACAGAGGAAGGTAGTAGAGCCTGCAAACTCCGACTCGGATTCTGAACTCGG CAACATTCCAAAGAAGACGGCCGCACCGAAAGGTAAAGGCCGAGGGGCAAAGAAAAGGAAAGCATCAGGC TCAGAAAATGAAGGTGATTATAACCCTGGCAGGAAACCATCTAAAACAGCCAGCAAGAAACCGAAGAAAA CGTCTTTTGATCAGGATTCAGATGTAGACATCTTCCCTTCAGACTTCACTTCTGAACCACCTGCTCTCCC ACGGACTGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGTCTGATGAAGAAGAAGACGTTGATTTT GCAATGTTTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217667 representing NM_001100858 Red=Cloning site Green=Tags(s) MAKSSLAGVDGALTWVNNAAKKEELETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLM WVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHK VEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKT SEAKIKHFDGEDYTCITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYV DLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVV KKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRY GVFPLRGKILNAREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKG LLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWEKHIENQKAWKIKYYKGLGTSTA KEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATK HLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGE QALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYD DNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQE LGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVK MTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGML GAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDAQNPHDDSS SDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQ EREDILAGMSGKAVKGKVGKAKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKV EFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPASTGKPNTKKVKKRNPWSDDESKS ESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEEDDADDDDDNNDLEELKVKASPITNDGEDEFVPS DGIDKDEYAFSPGKSKATPEKSSHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTTSVFAPSFGLKQ TDKVPSQTVAAKKGKAPSDAAAPKAKRAPRQRKVVEPANSDSDSELGNIPKKTAAPKGKGRGAKKRKASG SENEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDF AMFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100858
ORF Size	4842 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100858.1, NP_001094328.1
RefSeq Size	4845 bp
RefSeq ORF	4845 bp
Locus ID	361100
MW	182 kDa
Gene Summary	a nuclear enzyme that catalyzes the breaking and rejoining of two strands of duplex DNA [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.