Col11a1 (NM_013117) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217695 representing NM_013117 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTGGTCCAGGTGGAAAACGAAACGGTGGATCTGGGATCTCACCATCTCAACCCTCGTCTTGA CTTTTCTCTTCCAAGCCAGAGAGGTCAGAGGAGCTGCTCCAGTTGATATACTGAAAGCATTAGATTTTCA CAATTCTCCAGTGGGAATATCTAAAACAACAGGATTTTGCACAAGCAGAAAGAATTCTAAAGATCCAGAT ATCGCTTATAGAGTTACCGAGGAAGCCCAGATCAGTGCCCCAACGAAGCAGTTATTTCCAGGAGGAATTT TTCCGCAAGATTTTTCAATACTATTTACAATAAAACCCAAAAAAGGAACTCAAGCTTTCCTCTTGTCTCT CTACAATGAACATGGCATTCAGCAACTCGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTATTTGAAGAC CACACTGGAAAACCCACACCAGAAAACTATCCTCTCTTCTCAACAGTTAACATTGCTGATGGAAAGTGGC ACCGGGTAGCAATCAGTGTGGAGAAGAAAACTGTGACAATGATTGTCGATTGTAAGAAGAAAATCACAAA ACCCCTCGATAGAAGCGAGAGATCGATAGTCGATACCAACGGAATCATGGTATTTGGAACAAGAATTTTA GAAACAGATGTTTTCCAGGGTGATATTCAGCAGTTCCTGATCACTGGTGACCCCAAGGCAGCATATGACT ACTGTGACCATTACAGTCCCGACTGTGACTTAACATCCAAGGCCGCTCAGGCCCAGGAGCCGCACATAGA TGAGTATGCACCTGAGGATATAATCGAGTATGACTATGAATATGGGGAGACAGACTATAAAGAGGCTGAG AGTGTAACAGAGATGCCCACTGTCACTGAAGAAACAGTAGCACAAACTGAGGCAAATATTGTGGATGACT TTCAAGACTATAACTATGGAACGATGGAAACTTACCAGACTGAGTCTCCTCGGCGGGTATCAGGATCAAA TGAGCCAAATCCAGTTGAAGAAGGTTTTACTGAAGAATATCTAACCGGAGAGGATTATGATGTCCAGAGA AACATTTCCGAGGATATTCTGTATGGAAACAAAGGAATAGACGGCAGGGATTCTGATCTGCTGGTAGATG GAGATTTAGGTGAATATGATTTTTATGAATACAAAGAATATGAAGAAAGGACTACGACCTCCCCTAATGA AGAATTTGGCCCAGGTGTCCCAGCAGAAACTGATTTCACAGAAACAAGCATCAATGGCCATGGCGCATAT GGAGAGAAAGGGCAGAAGGGGGAACCTGCTGTAGTTGAACCCGGAATGCTTGTTGAAGGACCACCTGGGC CAGCTGGACCAGCGGGTCTTATGGGTCCTCCAGGACTACAAGGTCCTTCTGGGCTCCCCGGCGACCCTGG TGATAGGGGCCCACCAGGACGCCCTGGCTTACCAGGGGCTGATGGCTTACCCGGTCCTCCAGGAACCATG TTAATGTTACCATTCCGCTATGGGGGTGACGGCTCCAAAGGACCAACCATCTCTGCGCAGGAAGCCCAGG CTCAGGCGATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGCCCTCCTGGCCCAATGGGTCTTACCGGAAG ACCAGGTCCTGTGGGGGGTCCTGGTTCAGCTGGAGCCAAAGGTGAGAGTGGGGATCCAGGTCCTCAGGGT CCTCGAGGTGTCCAAGGTCCTCCTGGTCCAACCGGAAAACCTGGAAAGAGGGGCCGTCCAGGTGCCGATG GAGGAAGAGGCATGCCAGGAGAACCTGGGTCTAAGGGAGACCGAGGGTTTGATGGACTTCCAGGTCTGCC AGGTGACAAAGGTCACAGGGGGGAAAGAGGTCCACAAGGTCCACCTGGCCTCCCCGGCGATGATGGAATG AGGGGAGAAGATGGAGAGATCGGGCCCAGGGGTCTTCCAGGGGAAGCTGGTCCACGAGGCTTGCTGGGAC CAAGGGGCACTCCAGGACCTCCTGGGCAGCCTGGTATTGGAGGTATAGATGGCCCCCAAGGACCAAAAGG AAACATGGGTCCTCAAGGGGAGCCTGGACCACCAGGTCAGCAAGGAAATCCAGGACCTCAAGGACTTCCT GGTCCACAAGGCCCAATTGGTCCTCCTGGAGAAAAAGGACCACAAGGAAAACCAGGGCTTGCAGGACTTC CTGGTGCCGATGGACCTCCTGGTCATCCTGGGAAAGAAGGCCAGTCTGGAGAAAAGGGTGCCCTGGGTCC TCCTGGTCCTCAGGGACCTATTGGCTATCCCGGTCCCCGTGGAGTAAAGGGAGCAGATGGTGTCAGAGGT CTCAAGGGCTCTAAAGGAGAAAAGGGTGAAGATGGCTTTCCAGGATTCAAAGGTGACATGGGTCTTAAAG GTGACAGAGGCGAAGTGGGTCAAGTTGGTCCAAGAGGAGAAGATGGCCCTGAAGGCCCCAAAGGCCGTGC AGGCCCAACTGGAGACCCAGGTCCCTCTGGCCAAGCAGGAGAGAAGGGAAAACTTGGGGTTCCAGGATTG CCAGGATATCCAGGAAGACAAGGTCCAAAGGGATCCACTGGATTTCCTGGATTTCCAGGTGCCAACGGGG AGAAAGGTGCCCGGGGAATTGCTGGCAAACCAGGCCCCCGAGGACAACGTGGTCCAACGGGTCCTCGAGG TTCCAGAGGCGCCAGAGGTCCCACAGGAAAACCTGGGCCGAAGGGTACATCAGGTGGTGATGGCCCTCCT GGCCCACCAGGCGAAAGGGGCCCTCAAGGACCTCAAGGTCCAGTTGGATTCCCTGGACCAAAAGGCCCCC CTGGCCCTGCTGGGAAAGATGGGCTACCAGGACACCCTGGACAACGTGGAGAGACTGGATTTCAAGGAAA GACCGGTCCCCCTGGGCCAGGAGGTGTGGTAGGACCACAGGGACCAACTGGTGAGACTGGCCCGATAGGT GAACGTGGGCATCCTGGCACTCCTGGTCCTCCGGGCGAGCAAGGACTTCCTGGTGCTGCAGGGAAAGAAG GTGCAAAGGGTGATCCTGGTCCTCAAGGTATCTCAGGGAAAGATGGACCAGCAGGAATCCGTGGTTTCCC AGGTGAAAGAGGCCTTCCTGGAGCCCAGGGCGCACCTGGGTTGAAAGGAGGAGAAGGTCCCCAAGGCCCG CAGGGTCCAATTGGCTCTCCTGGAGAGCGAGGCTCTGCAGGCACAGCAGGCCCAATTGGTTTGCCAGGAC GTCCAGGCCCTCAGGGCCCTCCTGGTCCAGCTGGAGAAAAAGGTGCTCCGGGAGAAAAGGGTCCTCAGGG GCCTGCAGGTAGAGATGGGGTCCAAGGCCCTGTGGGTCTCCCAGGGCCTGCTGGTCCTGCTGGCTCTCCT GGAGAAGATGGAGACAAGGGTGAAATTGGTGAACCAGGCCAAAAGGGCAGCAAAGGTGACAAAGGCGAAA ATGGTCCTCCTGGTCCCCCGGGTCTTCAAGGCCCTGTTGGTGCTCCTGGAATTGCTGGAGGAGATGGTGA GGCAGGTCCCCGAGGACAGCAGGGAATGTTTGGACAAAAAGGTGATGAGGGTGCACGAGGTTTCCCAGGA CCTCCTGGGCCCATAGGTCTTCAGGGGTTGCCAGGCCCACCAGGTGAAAAGGGCGAAAACGGAGACGTTG GCCCTATGGGTCCACCAGGTCCTCCTGGCCCACGAGGCCCTCAAGGTCCCAATGGAGCTGATGGACCACA AGGACCCCCAGGCTCTATTGGTTCAGTTGGTGGTGTGGGAGAAAAGGGTGAACCAGGAGAAGCAGGGAAC CCAGGACCCCCTGGAGAAGCCGGATCAGGTGGTCCCAAAGGAGAGAGAGGAGAGAAAGGAGAAGCTGGGC CCCCTGGTGCTGCGGGTCCTCCTGGTATTAAGGGGCCACCAGGTGATGATGGACCCAAGGGGAATCCGGG ACCTGTTGGGTTTCCTGGAGATCCTGGTCCACCTGGAGAACCTGGTCCTGCAGGGCAAGATGGTGTTGGA GGTGACAAGGGTGAAGATGGAGATCCAGGACAACCAGGTCCCCCTGGTCCATCCGGTGAAGCTGGCCCTC CAGGTCCACCTGGAAAGAGAGGTCCGCCTGGAGCTTCCGGTTCCGAAGGAAGGCAAGGAGAGAAAGGTGC TAAGGGAGAAGCTGGAGCTGAAGGACCTCCTGGGAAAACTGGCCCGGTCGGACCTCAGGGTCCTTCTGGA AAGCCTGGTCCAGAAGGTCTTCGAGGCATCCCTGGTCCTGTGGGAGAACAAGGTCTCCCCGGTGCTGCTG GCCAAGATGGACCCCCTGGTCCTCTGGGACCTCCTGGTTTACCTGGTCTCAAAGGTGATCCTGGCTCCAA GGGTGAAAAGGGACATCCTGGTTTAATTGGATTGATTGGCCCTCCAGGAGAACAAGGGGAAAAGGGTGAC AGAGGACTTCCTGGGACTCAAGGTTCTCCAGGAGCCAAAGGTGATGGGGGCATCCCAGGTCCTGCTGGTC CCATAGGTCCTCCTGGTCCTCCAGGATTACCAGGTCCTGCTGGTCCGAAGGGTAACAAAGGATCTTCTGG ACCTACTGGCCAGAAGGGTGACAGTGGTATGCCAGGACCTCCTGGGCCTCCAGGTCCTCCTGGAGAAGTC ATACAGCCATTACCTATTTTGTCACCGAAGAAAACAAGAAGACACACTGAAAGCATCCAGGCCGACGCAG GTGATAATATTCTTGACTACTCAGATGGCATGGAGGAAATATTTGGTTCCCTCAATTCTCTGAAACAAGA CATTGAACACATGAAGTTTCCCATGGGTACACAGACCAACCCAGCACGAACATGCAAAGACCTGCAGCTC AGCCACCCCGACTTCCCAGATGGTGAATATTGGATTGATCCTAACCAAGGCTGCTCAGGTGATTCCTTCA AAGTTTACTGTAATTTCACAGCTGGTGGTGAGACGTGCATCTATCCAGACAAAAAATCTGAGGGAGTAAG ACTTTCATCTTGGCCGAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGAGGAAAACTGCTTTCC TATTTAGATGTGGAAGGCAATTCCATCAATATGGTACAAATGACATTCCTGAAACTCCTGACTGCCTCTG CCAGACAAAACTTCACCTACAATTGCCACCAGTCAACTGCTTGGTATGACGTCTTATCAGGAAGTTATGA CAAAGCACTTCGGTTTCTGGGATCTAATGATGAGGAAATGTCCTACGAGAACAACCCACACATCAAAGCT TTGTATGACGGCTGTGCGTCCCGAAAAGGCTATGAAAAGACAGTGATTGAGATCAATACTCCGAAAATTG ATCAAGTACCCATCATTGATGTAATGATCAATGATTTTGGTGATCAGAATCAGAAGTTTGGATTTGAAGT TGGTCCAGCTTGCTTTCTTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217695 representing NM_013117 Red=Cloning site Green=Tags(s) MEPWSRWKTKRWIWDLTISTLVLTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTSRKNSKDPD IAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFED HTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRIL ETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAE SVTEMPTVTEETVAQTEANIVDDFQDYNYGTMETYQTESPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQR NISEDILYGNKGIDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAY GEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTM LMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQG PRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGM RGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLP GPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRG LKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGL PGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPP GPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIG ERGHPGTPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGP QGPIGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSP GEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEAGPRGQQGMFGQKGDEGARGFPG PPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGGVGEKGEPGEAGN PGPPGEAGSGGPKGERGEKGEAGPPGAAGPPGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVG GDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSG KPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGD RGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEV IQPLPILSPKKTRRHTESIQADAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQL SHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRLSSWPKEKPGSWYSEFKRGKLLS YLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSTAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKA LYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013117
ORF Size	5412 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013117.1, NP_037249.1
RefSeq Size	7673 bp
RefSeq ORF	5415 bp
Locus ID	25654
UniProt ID	P20909
MW	181 kDa
Gene Summary	subunit of heterotrimeric collagen XI molecule which is expressed in developing cartilage and plays a role in regulation of collagen fibril diameter size; alternatively spliced as six different isoforms; may be a substrate for BMP-1 [RGD, Feb 2006]

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