EHBP1 (AK000828) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HPC12; NACSIN
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK000828, the custom clone sequence may differ by one or more nucleotides GAGGTGCGGCGGGGGAGCCCTCCAGAATACCATCATATAGCCCCTGAGGTGGCATGGTGATGTCTCCATG AGGGAACCCCTTCCCACTCATCCTGTCACGTATATCATAGTGTTCTTGACTGGGCCATTCATCTAAGATG GGATTTACCCTGTGAAACAGGGAGAAGACTTATGGACCCCAAGCATCATTTCGAGTTGTAGTTGAGTTTT TAAAAGACATACATGCAAAGTTCCTTTGCTTTGGACCCTCTGCATTATTAAAGCTGCTGTATTGCTAACC CAGAACTGCTCCAGTGTCTTGACTGATCATCATGGCTTCAGTTTGGAAGAGACTGCAGCGTGTGGGAAAA CATGCATCCAAGTTCCAGTTTGTGGCCTCCTACCAGGAGCTCATGGTTGAGTGTACGAAGAAATGGCAAC CAGATAAACTGGTGGTAGTTTGGACCAGAAGAAGCCGAAGGAAGTCTTCTAAGGCACATAGCTGGCAGCC TGGAATAAAAAATCCCTATCGTGGTGTTGTTGTGTGGCCTGTTCCTGAAAACATTGAAATCACTGTAACA CTTTTTAAGGATCCTCATGCGGAAGAATTTGAAGACAAAGAGTGGACATTTGTCATAGAAAATGAATCCC CTTCTGGTCGAAGGAAAGCTCTTGCTACTAGCAGCATCAATATGAAACAGTATGCAAGCCCTATGCCAAC TCAGACTGATGTCAAGTTAAAATTCAAGCCATTATCTAAAAAAGTTGTATCTGCCGCTCTTCAGTTTTCA TTATCTTGCATTTTTCTGAGGGAAGGAAAAGCCACAGATGAAGACATGCAAAGTTTGGCTAGTTTGATGA GTATGAAGCAGGCTGACATTGGCAATTTAGATGACTTCGAAGAAGATAATGAAGATGATGATGAGAACAG AGTGAACCAAGAAGAAAAGGCAGCTAAAATTACAGAGCTTATCAACAAACTTAACTTTTTGGATGAAGCA GAAAAGGACTTGGCCACCGTGAATTCAAATCCATTTGATGATCCTGATGCTGCAGAATTAAATCCATTTG GAGATCCTGACTCAGAAGAACCTATCACTGAAACAGCTTCACCTAGAAAAACAGAAGACTCTTTTTATAA TAACAGCTATAATCCCTTTAAAGAGGTGCAGACTCCACAGTATTTGAACCCATTCGATGAGCCAGAAGCA TTTGTGACCATAAAGGATTCTCCTCCCCAGTCTACAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK000828 unedited TTTTTAAACACGNACTCACGATAGGGTTTAAACCCCAATTCGGCACGAGGGCCAGACTCT TTCCACAGCGGGTATTAGAGCCCTCCAGAATACCCATCATATAGCCCCTGAGGTGGCATG GTGATGTCTCCATGAGGGAACCCCTTCCCACTCATCCTGTCACGTATATCATAGTGTTCT TGACTGGGCCATTCATCTAAGATGGGATTTACCCTGTGAAACAGGGAGAAGACTTATGGA CCCCAAGCATCATTTCGAGTTGTAGTTGAGTTTTTAAAAGACATACATGCAAAGTTCCTT TGCTTTGGACCCTCTGCATTATTAAAGCTGCTGTATTGCTAACCCAGAACTGCTCCAGTG TCTTGACTGATCATCATGGCTTCAGTTTGGAAGAGACTGCAGCGTGTGGGAAAACATGCA TCCAAGTTCCAGTTTGTGGCCTCCTACCAGGAGCTCATGGTTGAGTGTACGAAGAAATGG CAACCAGATAAACTGGTGGTAGTTTGGACCAGAAGAAGCCGAAGGAAGTCTTCTAAGGCA CATAGCTGGCAACCTGGAATAAAAAATCCCTATCGTGGTGTTGTTGTGTGGCCTGTTCCT GAAAACATTGAAATCACTGTAACACTTTTTAAGGATCCTCATGCGGAAGAATTTGAAGAC AAAGAGTGGACATTTGTCATAGAAAATGAATCCCCTTCTGGTCGAAGGAAAGCTCTTGCT ACTAGCAGCATCAATATGAAACAGTATGCAAGCCCTATGCCAACTCAGACTGATGTCAAG TTAAAATTCAAGCCATTATCTAAAAAAGTGTATCTGCCGCTCTTCATTTTCATTATCTTG CATTTTTCTGAGGGAAGAAAAGCCCAGATGAAGACATGCAAGTTTGCCC
Restriction Sites	NotI-NotI
ACCN	AK000828
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK000828.1, BAA91391.1
RefSeq Size	1243 bp
RefSeq ORF	1243 bp
Locus ID	23301
Gene Summary	This gene encodes an Eps15 homology domain binding protein. The encoded protein may play a role in endocytic trafficking. A single nucleotide polymorphism in this gene is associated with an aggressive form of prostate cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

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