PDE12 (AK055769) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK055769, the custom clone sequence may differ by one or more nucleotides CAAACTATATCACTTGTTGTCCATTTTTAATCTTTCTCTAACATACCCTAACTTCTTCTTGAAAATGCTT AATTTTCTCTGTTTTGAGATGTAAATTTGCTATCCTGTTTTTCTTAAAACTTGGTAAGATCTTCAGCCAT GAAGGACAGACAAACTGTAACCTTTCCATTTACAAAGCTGCATTTGAATTCAACTGTCCTTTTAAACTAA GGAGTTTTACTGGTCTCGTGGCTAAAATTTTAAAGATGTAGGCCAGGTGCAGTGGCTCATGCCTGTAATC CTATCACTTTTGGAGACCAAGGCGGGGATGGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAAC ATGATGAAACTCCATCTCTGCTGAAAATACAAAAATTAGTCAGGCATGGTGGCACACACCTGTGGTCCCA GCTACTCGGGAGGCTGAGGCAAGAGAATGGCTTGGGCCCGGGCGACGGAAGTTGCAGTGAGGCGAGATTG CACCATTGCACTCCAGCCTGGGCAATAAGAGTGAAACTCTGTCTCAAAAAAAGAAAAAATGATATAAAGT ATTTATTTGTGTTTATATTTTATGGATACGTGTCTATGTTTATAGATTGCCTGTATGGTACCAACTTGAC ATAAATAAATTAGTACTCATAAATTAAGTAAATAAGCCCAAACGCTTTTCAAGTTCACAGGGCATTAGTA ATCTGTGGTAAATGAAGATAGTTTAAAATTTGGGGTAAAATAAAATAGAAACATTTTCTGAATTTAATTT AGATATTTTTCTGGGTCTATTTGTTAGACAGGTTTACACTATCTCTGCTATTTTTTTAAGGACCTAAAAC TGAATGGACATAGAAAATTGAGGAAAGAAATAAAACCGTAACAATTATAAGAGGTTATAAAAGGTTCATG GAACCTTTTTATGTCTTTTCTTGTGCAGTCAAAACCAATTGCAGGCTGGATGCAGTAGCTCACACTTGTA ATCCCAGCACTTTGGGAGGCTGAGATGGGCAGATCACTTGAGCTCAGGAGTTTGAGACCATCCTGGGCAA CATAGTGAAACTCTCTCACTACCAAAGTACAAAAGGAATTAGCTGGGCATGATGGTACACACTTGTAGTC CCAGCTACTTGGGGGACTGAGATGGGAAAATTGCTTGAGCCCGGGAGTCAAAGGTTGCAGTAAGCCAAGA TTGCACCACTACACTCCAGCCTGGGTGACAAAGTGAGACCCTGTCTCAAAAAAAAACAAAAAAAGGAAAA GAAAACAATTGCAATTGGATTGATCTGTTTATAAGGCTTTATTAAAATTAGCTTTAGCATTTATAATACA CTGGCACAAAGTTAGAATTTGGATTTCTCTTTTGAACAAGATTTTTGTATACATTAATAAGAAATAGTAA AAGATTTTTGTTTACCTTTTGAGTAAACTGCAAAAAATATTAGTAATAAGAGGAGAGAATTTCCCTAATG CTGTTTTTATTATGTCTTTTGATTGTTTGGAAAACTGAATCTCCCCTCTGTCAAAGAGTAAAATTTTTGC TTTTTGAAATTTTTGAATTATCAGTTTGGCTAAATGAATAACTATTATCTTACAGTGACCTGTAATCTTA TTTTGATCAAGTGTTTTAAACCTTTGATATTTGACAAACTTTCCAAAATCAAATGTAAAATTCTAACTTA AATGTTTTCGACCTCAAGCTAACTTTTGGGCATTAAAGCCCTTGGAAGTCCAAATAGGGCCCCTGGAAGT CCAAAAGAAACATATTAGGCTAATTTGGTATATTAAAATCATACAGGAAGCTGATCAAATAAAAAATGTT GTTTAATTTTCTTTGAGTTGTATTTGTATAAATCTGTTATTAATATGCATCCCAAAATTGTATCATATTG CTAAAATGTTGATAGATCTTGTTATATGTTATTGGTAATAATTGTTATCCTGTTAAATTATTGTATACCA CAGAAATAATGAAATTTCTTTGTCAATTGCATCAGTAATCATGGCTCTTCTAAGTCTTTTGTCATTCACA GATGATTATTGTTTTACTTGGATTCTTTTCAAAAGTGGTTTGTAATTGGCTACAGCCTAAAATCTGCTTG TTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK055769 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGACCACTGTACTCCAGCCTG GGCAACAGAGTGATACTCTATCTCAAAAAAAAAAGAAAGAAAGAAAGAAAAGAAAAAGAA GCTTACAATCATGGTGGAAGGCGAAGAGGAGGAGCAGGCATATCACATGGCCAAAAAGGG AGCAAGAGAAAGGTGAGAAAGACACTAGACTCTTTTTAAAACCAGCTCTCACATGAGCTA ATGGAATAAGAACTCACTCATTACCACAAGGACAGAACCCAGCCATTCATGAGGGACCTG CCACCATGACCCAAAAACCTCCTACTTGACCCAACCTCCAACACTGGGGATAACATTTCA ACATGAGATTTGAAGAGGACAAATACTCAAACTATATCACTTGTTGTCCATTTTTAATCT TTCTCTAACATACCCTAACTTCTTCTTGAAAATGCTTAATTTTCTCTGTTTTGAGATGTA AATTTGCTATCCTGTTTTTCTTAAAACTTGGTAAGATCTTCAGCCATGAAGGACAGACAA ACTGTAACCTTTCCATTTACAAAGCTGCATTTGAATTCAACTGTCCTTTTAAACTAAGGA GTTTTACTGGTCTCGTGGCTAAAAATTTAAAGATGTANGCCAGGTGCAGTGGCTCATGCC TGTAATCCTATCACTTTTTGAGACCAANGCGGNGATGGATCACCCTGAGTCANGAGTTCA AGACCAGCCTGGCCAACATGATGAAACTCCATCTCTACTAAAAATACAAAATANTCAGGC ATGGTGGCACACACCCTGTATCCCAGCTACTTCCGAAGGCTGAGGGCAGAAAATGCTTTG ACCCCGNCNACCGNAATTTCANTGAGGCGAAATTGACCATTGCCCTCCAGCCTGGCAATA GAAGTGAACCTTGCTCAAAAAAGAAAAATGATTAAGTATTATTTGGGTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK055769 unedited GTTCTGCTATGGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGAAC AGCAGATTTTAGGCTGTAGCCAATTACAAACCACTTTTGAAAAGAATCCAAGTAAAACAA TAATCATCTGTGAATGACAAAAGACTTAGAAGAGCCATGATTACTGATGCAATTGACAAA GAAATTTCATTATTTCTGTGGTATACAATAATTTAACAGGATAACAATTATTACCAATAA CATATAACAAGATCTATCAACATTTTAGCAATATGATACAATTTTGGGATGCATATTAAT AACAGATTTATACAAATACAACTCAAAGAAAATTAAACAACATTTTTTATTTGATCAGCT TCCTGTATGATTTTAATATACCAAATTAGCCTAATATGTTTCTTTTGGACTTCCAGGGGC CCTATTTGGACTTCCAAGGGCTTTAATGCCCAAAAGTTAGCTTGAGGTCGAAAACATTTA AGTTAGAATTTTACATTTGATTTTGGAAAGTTTGTCAAATATCAAAGGTTTAAAACACTT GATCAAAATAAGATTACAGGTCACTGTAAGATAATAGTTATTCATTTAGCCAAACTGATA ATTCAAAAATTTCAAAAAGCAAAAATTTTACTCTTTGACAGAGGGGAGATTCAGTTTTCC AAACAATCNAAAGACATAATAAAAACAGCATTAATGGAAATTCTCTCCTCTTATTACTAA TATTTTTTTGCAGTTTACTCAAAAGGTAAACAAAAATCTTTTACTATTTCTTATTAATGT ATACAAAAATCTTGTTCAAAAGAGAAATCCAATTCTACTTTGTGCCCGTGTATTATAAAT GCTAAAGCTAATTTTAATAAAGCCTTATAACAGATCAATCCNATTGCCATTGGTTTCTTT TCCTTTTTTGTATNTTTTGAGACGGGTCTCACA
Restriction Sites	NotI-NotI
ACCN	AK055769
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK055769.1
RefSeq Size	2103 bp
RefSeq ORF	2103 bp
Locus ID	201626
Gene Summary	Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. Degrades triphosphorylated 2-5A to produce AMP and ATP (PubMed:26055709). Also cleaves 3',5'-phosphodiester bond of oligoadenylates (PubMed:21666256, PubMed:30389976, PubMed:26055709). Plays a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme increases cellular 2-5A levels and decreases viral replication in cultured small-airway epithelial cells and Hela cells (PubMed:26055709).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.