KIAA1731 (CEP295) (AK095218) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA1731
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102821 sequence for AK095218 edited (data generated by NextGen Sequencing) ATGGGGCAGCTCAGAGACTGGTTTCCTAATACACAAGACCTAGCAGGAAATGATCAAGAA AATATTAGGCATGCAGATAGGAACAACTCTGATGATAATCATTTGGCTTCAGAAGATACT AGTGCCAAGCAAAGTGGTAAACCAGGTATTTATGAAGACAGAGACCCCCTGCGAGTCTCA ATAAGCCGAGAACAAAGTTTCTTTGGGAGCCCACTGGCCCATGATCCGTTTAGTTGTCTT CAACTGGTTGGCCAAGAGAATGTCTGTGGTGATGACTATGATGAAGCAGTTAAGCTGAAG GAATCTGTTGTTGAAAATCATGCAGTGTTAAGTTATGCTGTGGAGGAAGAACATGCATAT TTGGGTCCAACTGTGAAGCCAGATGATAAGGCTAAAACACTGTCTTATGAACCATTATCT TCAGCAACTGTTTCCACTGGGAGCCTTTTAAGTTATGAAAACACAGATTTGAGCCTTACA GATCCAGAGTCATTTTCAGAGCACATGGATGATAGCAAGCAAGAATCTACCACCAGTAAA GAAGAGGAAACAAATATTATAAGTTCCATAGTTCCTTCAACACAAGATATTTATCAGCGG CAGAACTCTTCAGACGTTCATAAATCTCTGTTGCCTGCAGTGGATGAAACTACATGTGGT CACACACACTTTCAGCAAATGATAGACAAGTACATTAATGAAGCAAATTTGATACCTGAA AAAACAGATTTGCAAGAATTGGAACACATTTTTCCTAATTTGCATCATCAGCTGTTTAAA CCCTTAGAACCACATCCAGATTTTGACTTATCATCATCATCCTCTGGGATTTCTCCAGAC AACAGAGACTTTTACCAGAGATCAGATTCTTCATCTGAAAGCCACTGTGCTACTGGATTA TCCAAAAGTACAGTTTATTTCACAGCACTGAGGAGGACCAGCATGCATTCTTCTCTTAAC ACAAGTCCGAATCAACAACCTGACACTAACTTGGCTCATGTTGGAGCTCACAGTTTTGCT ACAGAAAATATTATTGGGGGATCTGAACAATGTTTTGAACAGCTTCAGCCAGAATATTCT TCACAGGAGGAGAGCCAGCATGCTGATCTACCAAGTATTTTTAGCATTGAAGCAAGAGAT TCTTCCCAAGGCATGAAAAATCAGAACTATCCCTCTGAAGAACATACTGAAATATTACAA AACAAGAAAAAAATTGTTCATTTCCAGCTTTCTATAGGAAACTTAAGTTCAGTCTACAGT TCATCTGATGAAGCTAATGTATTTGATCAGTTAAATGTACAGCATAGCACTCCATGTGGT TCTAACTCTAGTGAGTGCTCAACAAAACACCAACTAGAAAGCAGAAAGGAAAGTATGGGC TTTGAAGAACTATCAAAAAGAGGGGTTGTTACAATGTTACAAAGTCAAGGACTCATTGAA GATAATAAAAATGAAACCTGTAGGGTTTTAGACATAAATCCACAGGTAGAGGAAACTGAC TCTCGATTATGTGTAAGAACAGTGGAGATGGGAACTTCAATTCAGGCACCATATTCCTTA ACTACTCAAAATGAAAAATATTTTGAGAATTCAGCTGAAACAGACATTCCAAAAATCACC AAAAAACTATCTCAACTAGGAGAATCAGAGCTTTTTGCAAGTTCTGGATCATTTTCATTA CAGAGCTCTATACCAGTCTGGGAAACAGAAACTGGCCATGGTATAATGGAAGAACCAGAA CTTACTTTAATAAGCACCACTGATACCAGTATTGCTGAAATGGATTTTGCAAATTTAACC CTAGAAGAGAAGAGCGAGAATGAAGCAAAATGCTTCTTTCAGGTGAGTGTGTTTCTGCCT CTTGTATCAGCAACAGAAGCCTCAGATTATCCAGCTGTATCAGAACTTTCCATAGAAAAA CCAAGGACAGCATCTACAGAAACCCCTCGCAGGCTTACACCTGTACCAGGGAGCTTACAA GAAGCATTTATAAAGAGGAAAAAATCATTTATGGAGAGATCCCACCAGAGGCAGAAAGAA ATAAGGAATAAAATTCATGTCTCTGAAAATTCTCAAATCAAAACAGTTAAAGAGAAACCA TCTATAAGTTCATCTGTGAGTCGTCTAAAGGGCGTGAATAAAGTCAGAGCATCTTTTCCT GAAGACAGAAAGACTACACAGGCTCTAAGGCACCAAAGGGGTCTAAGGTTATACAATCAA CTAGCTGAAGTGAAACAACAAAAGGAAGAAAAAACAAAACAAGAAGCTTATGCCCAAAAC AGAGCAAGGGCAAAAGAATTCCATAAGAAAACACTAGAGAAACTTCGAGCCAAAAATACA TGCTGA Clone variation with respect to AK095218.1 202 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095218 unedited NGGGAGTTCACTTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGACT TCAGAACAGTTGGATCTACAAANAGAAGTTCTGCATTATAGCCAGAAAGCCCAGGAAAAA TTGCTTGTACAGAGACAAACAGCATTGCAGCAGCAGATACAGAAACATGAAGAGACTTTG AAGGATTTCTTTAAAGACAGTCAGATAAGTAAGCCCACAGTTGAAAATGATTTAAAAACC CAGAAGATGGGGCAGCTCAGAGACTGGTTTCCTAATACACAAGACCTAGCAGGAAATGAT CAAGAAAATATTAGGCATGCAGATAGGAACAACTCTGATGATAATCATTTGGCTTCAGAA GATACTAGTGCCAAGCAAAGTGGTAAACCAGGTATTTATGAAGACAGAGACCCCCTGCGA GTCTCAATAAGCCGAGAACAAAGTTTCTTTGGGAGCCCACTGGCCCATGATCCGTTTAGT TGTCTTCAACTGGTTGGCCAAGAGAATGTCTGTGGTGATGACTATGATGAAGCAGTTAAG CTGAAGGAATCTGTTGTTGAAAATCATGCAGTGTTAAGTTATGCTGTGGAGGAAGAACAT GCATATNTTGGGTCCAACTGTGAAGCCAGATGATAAGGCTAAAACACTGTCTTATGAACC ATTATCTTCAGCAACTGTTTCCACTGGGAGCCTTTTAAGTTATGAAAACACAGATTTGAG CCTTACAGATCCAGAGTCATTTTCAGAGCACATGGATGATAGNCAGCAAGAATCTACCAC CAGTAAAGAAGAGGAAAACAATATTATAAGTCCATAGTTNCCTTCACACAAGATATTTAT CAGCGGCAGACTCTTNCAGACGTNATAAAATCTCTGTGCCTGCAGTGGATGAACTACTTG GGGTCAACACACTTTCAGCAATGATAGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095218 unedited NTTGGGTTTACTATGNNACCGCGGCCGCATNCTANGATCGGATTTTTTTTTTTTTTTTTT CCTCGAAAACAATTTATTGACTTTAAATAATTTTGTCTAATGCTACATATACACAATTAA AAAACCTTTACACTATTTCTAGAAAGTCAGCATGTATTTTTGGCTCGAAGTTTCTCTAGT GTTTTCTTATGGAATTCTTTTGCCCTTGCTCTGTTTTGGGCATAAGCTTCTTGTTTTGTT TTTTCTTCCTTTTGTTGTTTCACTTCAGCTAGTTGATTGTATAACCTTAGACCCCTTTGG TGCCTTAGAGCCTGTGTAGTCTTTCTGTCTTCAGGAAAAGATGCTCTGACTTTATTCACG CCCTTTAGACGACTCACAGATGAACTTATAGATGGTTTCTCTTTAACTGTTTTGATTTGA GAATTTTCAGAGACATGAATTTTATTCCTTATTTCTTTCTGCCTCTGGTGGGATCTCTCC ATAAATGATTTTTTCCTCTTTATAAATGCTTCTTGTAAGCTCCCTGGTACAGGTGTAAGC CTGCGAGGGGTTTCTGTAGATGCTGTCCTTGGTTTTTCTATGGAAAGTTCTGATACAGCT GGATAATCTGAGGCTTCTGTTGCTGATACAAGAGGCAGAAACACACTCACCTGAAAGAAG CATTTTGCTTCATTCTCGCTCTTCTCTTCTAGGGTTAAATTTGCAAAATCCATTTCAGCA ATACTGGTATCAGTGGTGCTTATTAAAGTAAGTTCTGGTTCTTCCATTATACCATGGCCA GTTTCTGTTTCCCACACGGGATAGAGCTCTGTAATGAAAATGATCCCGAACTTGCAAAAA GCCTGGATCTTCCAATTGAAAAAAGTTTTTGGGGGATTTTTC
Restriction Sites	NotI-NotI
ACCN	AK095218
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095218.1, BAC04502.1
RefSeq Size	2890 bp
RefSeq ORF	2346 bp
Locus ID	85459
Gene Summary	Centriole-enriched microtubule-binding protein involved in centriole biogenesis (PubMed:20844083, PubMed:25131205, PubMed:27185865). Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure (PubMed:27185865). Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles (PubMed:27185865). Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement (PubMed:25131205). Binds to and stabilizes centriolar microtubule (PubMed:27185865).[UniProtKB/Swiss-Prot Function]

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