C20orf77 (RPRD1B) (AK092567) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C20orf77; CREPT; dJ1057B20.2; NET60
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092567, the custom clone sequence may differ by one or more nucleotides ATTTGTACTTAGACAAAGAGGCAGCTGAACGTCTTTCAAAAACAGTAGATGAAGCATGTCTGTTACTAGC AGAATATAACGGGCGCCTGGCAGCAGAACTGGAGGACCGTCGCCAGCTGGCTCGGATGTTGGTGGAGTAT ACCCAGAATCAGAAAGATGTTTTGTCGGAGAAGGAGAAAAAACTAGAGGAATACAAACAGAAGCTTGCAC GAGTAACCCAGGTCCGCAAGGAACTGAAATCCCATATTCAGAGCTTGCCAGACCTCTCACTGCTGCCCAA CGTCACAGGGGGCTTAGCCCCCCTGCCCTCTGCTGGGGACCTGTTTTCAACTGACTAGGATGGGTGTCAT GTCCCAGATTTCTGTTTGTACCAGCAGAAAGAAGAGGGCAAGTCATGGTTGGAAATAACCTTCTAGCCCC TGGTTCTATCCCTTCTTCCGCCCAGCCCCCCAGCCTCAAGAAAGAACCTCAGACTCTGATTCTCCTCTTC AGCCTCTCATCTTGAGCACAGTTCAGAACAGTGGCGACTGGAATCTGGTTTATATTCATATTTGCAAAGA CTACAGACTTTTTCTCCCACTTCATATTTTCATGCCCCCCTGTTGGTTTTCCATTCTTAACTGTCTCCTT ATACCTAAGAAGTTATGAAAATCATGTGTACTTCTGGAAGCTTTCGAAAGAATCTTGTCCCTCATGACAG CATTTTATCATGAAAGCAGCTTCTCCTTTCTGGGCTGGGCTTGTTCAAGTTCGGTGTGGGCTTCCACTAA GGCACTTGTCCTGGAGACGTTGGCTTTCCCAGCTGCATCTGCCCCAAAAGGTTGTAGGCACAGCTGTCGT AGCGTTGCCATAAAGAGTTTGCCAAATCTCTGATCCTCCCTTTCCATTGCTTCTCCTAGTGATGCACGAA GATTAGGTGCATTTATTTTGTAAACAGATTGGAGAATCTAGCAATAAGATTCAAAGCTAATCTGGAGCAT AAAGGCACAGTTCAGAGACAGAATAACAGGGATCACAAGCATGAATTAAAAGGAATTTATTTGCTTCAAA TTCCTAGATACAACCTTCCCATGCTGCACTTCTCCACTGTCGGAGCACGTTCCGAAAAACAGAATGCCTT GATCCCTGGTGGGTGCGAAGGCAGTTGTTAGGGATGGCAGGCATTGGTGGGCTCCAAAAGATGAAGGCCC CACACACAGGTGTGCTGCATTTGGGATCTGTGTGGGTGTTTCTTGGACCCTTTCTTCTGGGAGTAGGGTA CACACTAACGTTTAATCCGCTGTCTGGGTGCATGTCCACAGTACGGTGGCTAAACTCGAACATCACTGCA AATAGGACGCTGAGCAGGTCCGTCTGTCATGTCACGCCACTGCACAGGTCCTTGTCCCCACACGACGGGG AGTACTTGCGTCAGATGTTATTGAATAGCTCGTCTCGGGCAGGGGAAGCGGGGAGTTGGGGATATTAATT GGGGGTTTTAATTCTATTATCATGTCAGCTGACATTATGACTATATAATGTAGTTAGAGACAATTTTTAT CTTGCTTATAGTAAAGGTTCAGCCTGCCAATTGTAAATCATTCTAATTTGGCAGGCTTATTTTTGACATT GGAAAGGGCAGAAAGCGATTTGCCCCAGTAGTGTAATAGGAGTTATAGACCAGAGGCTGAAACCCAAACT ATATAAAAAGGAATTCAGTGGAGGGGGCTTTGTAATCTCCATTAATTTGTGTTGCTACTTCCAGGATCAC CAAAAATTACATGTAATTTTACATGTTAAACACATTGAAACATAGGTAACCTATGTTTATAAAGCATAAC GGGCTTCCCTTCCAGAAGCTCTCCTGCTTGTCATGAAGTGAGAACAATGAAAAGTCATAGCAGATACTCA GTTTAACTCTGTGTAGAACCTAGTAGTGTTTGAGCTGTTATTCAGATTTGAATTCAGACTGTGTGTTGTT TGCTTATGGACACTGCCTGTCGTTCTGTCACTGTTAAACTAATGAGTCTATAAGGTTTTTCTTCCAGAGG CCATAGGTGACATCACTAAAATTGCAAGATAAATTGTAATCTTTGCTGCTGCTGCACTCCCCAACCTCTC CCCCACCCCCCGTGGTGTGCTGCTTTCTAGATGAGCGTGTTTTGGAGCAGGCCCATCTGGGACACTCTAT GCTTTCACCAAGGAAGTGCGATCTGAGCAGCCACAATCCAGCCAAAAGAGGATCGTAGATATTTGCTCTG ATCAACTAGATGAAAATATAGCAGAATGGATTTAGCCCACTGCTCTGTTTTATCCAACTGAGTCTCTGAC CAGCAATTGGTGCATAATTATTACAGCAAAAGTTAAGAAATGAAACTGTAGCAATTATGTAAATGAATGT GTTGGCCTCTTAATACCTGTTACTAGTGGACTTCCTGTGAGGAAGTTAGTTTTTTGTTTTGATGAAATGC TTTCGTTTTTTAAATCTTAATTCTGCTGTCCACATCCTCCCAAAGTGTGCTTACTTCATTTGTTTAATTT AAATGAACTTTCCTCCTTGTATGTATGAGGTGACTTGGTGGGTGGGGTGGGTGGTTTTTGTTTTTGTGTT TTTTCTTTCTTAGGGCATCTGTAGGCCTCAAAGGACCTTTCCTTTAGGTCATATTCCTCAGAAAGTCTTC AATCTTCCCTTGTTTTTGTTTGTTTGTTTTTCTTAAAGAATATTTTCAAAGCTTAAATTTGTATATTAAT TTAGGACTATTTAGAAGTATAGGCTGTCGTTGGCGGCAGCAGTATATTCTGAAATGTCTCATAGATATAT ATTTTTGAATAAAGATGGTGTTGTTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092567 unedited AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGGGAAATAA CCTTCTAGCCCCTGGTTCTATCCCTTCTTCCGCCCAGCCCCCCAGCCTCAAGAAAGAACC TCAGACTCTGATTCTCCTCTTCAGCCTCTCATCTTGAGCACAGTTCAGAACAGTGGCGAC TGGAATCTGGTTTATATTCATATTTGCAAAGACTACAGACTTTTTCTCCCACTTCATATT TTCATGCCCCCCTGTTGGTTTTCCATTCTTAACTGTCTCCTTATACCTAAGAAGTTATGA AAATCATGTGTACTTCTGGAAGCTTTCGAAAGAATCTTGTCCCTCATGACAGCATTTTAT CATGAAAGCAGCTTCTCCTTTCTGGGCTGGGCTTGTTCAAGTTCGGTGTGGGCTTCCACT AAGGCACTTGTCCTGGAGACGTTGGCTTTCCCAGCTGCATCTGCCCCAAAAGGTTGTAGG CACAGCTGTCGTAGCGTTGCCATAAAGAGTTTGCCAAATCTCTGATCCTCCCTTTCCATT GCTTCTCCTAGTGATGCACGAAGATTAGGTGCATTTATTTTGTAAACAGATTGGAGAATC TAGCAATAAGATTCAAAGCTAATCTGGAGCATAAAGGCACAGTTCAGAGACAGAATAACA GGGATCACAAGCATGAATTAAAAGGAATTTATTTGCTTCAAGTTCCTAGATACAACCTTC CCATGCTGCACTTCTCCACTGTCGGAGCACGTTCCGAANAACAGATGCCTTGATCCCTGG TGGGTGCGAAGGCAGTTGTTAGGGATGGCNAGCATTGGTGGGCTCCAAAGATGACGNCCA NACACAGTGTGCTGCTTTGGGACTGGNTGGNTGNTCTGCACCTTTCTCTGGAGTAGCAAN ACTAACGTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092567 unedited CGGTTTATCTATGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTTTAA ACAACACCATCTTTATTCAAAAATATATATCTGTGAGACATTTCAGAATATACTGCTGCC GCCAACGACAGCCTATACTTCTAAATAGTCCTAAATTAATATACAAATTTAAGCTTTGAA AATATTCTTTAAGAAAAACAAACAAACAAAAACAAGGGAAGATTGAAGACTTTCTGAGGA ATATGACCTAGAGGAAAGGTCCTTTGAGGCCTACAGATGCCCTAAGAAAGAAAAAACACA AAAACAAAAACCACCCACCCCACCCACCAAGTCACCTCATACATACAAGGAGGAAAGTTC ATTTAAATTAAACAAATGAAGTAAGCACACTTTGGGAGGATGTGGACAGCAGAATTAAGA TTTAAAAAACGAAAGCATTTCATCAAAACAAAAAACTAACTTCCTCACAGGAAGTCCACT AGTAACAGGTATTAAGAGGCCAACACATTCATTTACATAATTGCTACAGTTTCATTTCTT AACTTTTGCTGTAATAATTATGCACCAATTGCTGGTCAGAGACTCAGTTGGATAAAACAG AGCAGTGGGCTAAATCCATTCTGCTATATTTTCATCTAGTTGATCAGAGCAAATATCTAC GATCCTCTTTTGGCTGGATTGTGGCTGCTCANATCGCACTTCCTTGGTGAAAGCATAGAG TGTCCCAGATGGGCCTGCTCCAAAACACGCTCATCTAGAAAGCAGCACACCACGGGGGGT GGGGANNAGGTTTNGGNNAAATTNACACNCCNNCAAAAATTACCATTTTAATCTTGGCAA TTTTAAGGAATGTCACCTATGGGCCCCTGGGAAGAAAAAACCTTATAGACCTCCTTTAAT TTAAACGGGTGACAGAACCC
Restriction Sites	NotI-NotI
ACCN	AK092567
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092567.1
RefSeq Size	2826 bp
RefSeq ORF	2826 bp
Locus ID	58490
Gene Summary	Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.[UniProtKB/Swiss-Prot Function]

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