BOD1L (BOD1L1) (AF528529) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BOD1L; FAM44A
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF528529, the custom clone sequence may differ by one or more nucleotides ATGGCCACCAACCCACAGCCGCAGCCGCCTCCTCCGGCGCCGCCGCCTCCCCCGCCGCAGCCGCAGCCGC AGCCACCGCCGCCGCCGCCGGGCCCCGGGGCTGGCCCCGGCGCGGGCGGGGCGGGCGGCGCGGGTGCGGG CGCCGGGGACCCGCAGCTCGTGGCCATGATCGTGAACCACCTCAAGAGCCAGGGGCTCTTCGACCAGTTC CGCAGAGACTGCCTGGCCGACGTGGACACCAAGCCTGCGTATCAGAATCTGAGACAGCGTGTTGACAACT TTGTTGCAAATCACTTGGCAACTCACACATGGAGTCCGCATCTCAATAAGAACCAGCTAAGAAACAACAT TAGACAACAAGTCCTCAAATCAGGAATGTTGGAGTCTGGTATTGACCGAATTATTTCTCAGGTTGTGGAC CCAAAGATCAACCACACATTCAGACCTCAGGTAGAGAAAGCTGTGCATGAGTTTTTGGCCACGCTAAATC ACAAAGAGGAAGGAAGTGGCAACACAGCTCCCGATGATGAGAAACCAGACACTTCCCTTATTACACAAGG TGTTCCTACTCCTGGGCCCAGTGCTAATGTAGCCAATGATGCCATGTCGATATTGGAAACCATAACTTCT CTTAACCAAGAAGCCAGTGCTGCTAGGGCTTCAACAGAAACATCAAATGCCAAGACCAGTGAGAGAGCGT CAAAAAAACTTCCATCTCAGCCAACCACTGATACTAGTACTGACAAAGAAAGAACTTCAGAGGACATGGC TGATAAAGAAAAATCTACAGCTGACTCTGGAGGTGAAGGACTGGAAACAGCCCCAAAGTCTGAAGAGTTC AGCGACCTCCCCTGTCCAGTCGAAGAAATTAAAAATTACACAAAAGAGCATAATAATTTAATTCTGCTAA ATAAGGATGTTCAACAGGAAAGCAGTGAGCAAAAAAATAAATCAACAGACAAAGGTGAAAAGAAGCCAGA CAGCAATGAGAAAGGAGAAAGAAAGAAAGAAAAGAAGGAAAAGACTGAAAAGAAATTTGATCACTCAAAA AAGAGTGAAGATACACAGAAAGTTAAAGATGAAAAACAAGCAAAGGAAAAAGAAGTAGAGAGTTTAAAAC TTCCTTCAGAAAAGAACAGTAATAAAGCTAAAACTGTTGAAGGGACAAAAGAAGATTTCTCTTTGATAGA TTCTGATGTGGATGGACTTACAGACATCACAGTTAGCTCTGTTCATACCAGTGACCTTTCATCTTTTGAA GAAGATACTGAGGAGGAAGTTGTAACGTCTGATAGCATGGAAGAAGGAGAGATTACGTCAGATGATGAAG AGAAGAACAAACAGAATAAAACAAAAACTCAAACTAGTGATTCTAGTGAAGGAAAAACAAAAAGTGTACG GCATGCGTATGTCCACAAACCATATCTTTACTCAAAATACTATAGTGATTCTGATGATGAGCTTACTGTA GAACAACGACGACAGTCCATTGGTATTTTGTGGTTTTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF528529 unedited GTCACCTTTTGTAATACGACTTACTATAGGGCGGCCGCGTAATTCGCACGAGGGTTCCGT AACAACATCCCGTTGGCTTCCCTCAGGCGGCGGGACCAGTGCAGCCGCCGCCTCCCAGGA TCGTCCGCCGGTCAGGGCCCTTGCCCTCCCCGGCACAGGCCACCATGGCCACCAACCCAC AGCCGCAGCCGCCTCCTCCGGCGCCGCCGCCTCCCCCGCCGCAGCCGCAGCCGCAGCCAC CGCCGCCGCCGCCGGGCCCCGGGGCTGGCCCCGGCGCGGGCGGGGCGGGCGGCGCGGGTG CGGGCGCCGGGGACCCGCAGCTCGTGGCCATGATCGTGAACCACCTCAAGAGCCAGGGGC TCTTCGACCAGTTCCGCAGAGACTGCCTGGCCGACGTGGACACCAAGCCTGCGTATCAGA ATCTGAGACAGCGTGTTGACAACTCTGTTGCAAATCACTCGGCAACTCACACATGGAGTC CGCATCTCAATAAGAACCAGCTAAGAAACAACATTAGACAACAAGTCCTCAAATCACGAA TGTTGGAGTCTGGTATTGACCGAATTATTTCTCACGTTGTGGACCCAAAGATCAACCACA CATTCAGACCTCAGGTAGAGAAAGCTGTGCATGAGTTTTTGGCCACGCTAAATCACAAAG AGGCATGAAGTGGCAACACAGCTTCCCGATGATGAGAAACCAGACACTTCCCTTATTACA CAAGGTGTTGCCTACTCCTGGGGTCCAGTGCTTATGTAGCCAATGATGGCCCTGTCGATA TTGCGAAACCATAACCTTCTTTTAACCACAAAGTCAGAGCTGCTTGGGCTTCAACAGAAC CACAAATGCCCAGACCATTGAGAGACCGGCAAAAACTTTCCTTTAGCCAACCCTGGATCT TGTCCTGACAAAAAGAACTTCGAAGCCATGCCTGAGATACAAAAACCCACACTGGCTCTG GAGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF528529 unedited AGNGATCAAGTTTTTTTTTTTTTTTTTTGCTTAAGGCTACTTTTTTTTCTAAAACTTTCC GTTCTTTAAGGACTTCTTTAATTCTGGCGGCTTTAGGTTCCAAACTCTTTGTTGATGATT CTTCTAAGTCCACACTACTCCTGCCTTGACCCTTGGTTTTTGAAGACTTTGTCTTTTCTG CTTTCTGTTTTCGTTTTTCTTCAAGTTTTTCTCTATTGATTTGCCTTCTTAAAAGCCTCT CTTCTTTTTCTTTGGCAATGGACTGTCGTCGTTGTTCTACAGTAAGCTCATCATCAGAAT CACTATAGTATTTTGAGTAAAGATATGGTTTGTGGACATACGCATGCCGTACACTTTTTG TTTTTCCTTCACTAGAATCACTAGTTTGAGTTTTTGTTTTATTCTGTTTGTTCTTCTCTT CATCATCTGACGTAATCTCTCCTTCTTCCATGCTATCAGACGTTACAACTTCCTCCTCAG TATCTTCTTCAAAAGATGAAAGGTCACTGGTATGAACAGAGCTAACTGTGATGTCTGTAA GTCCATCCACATCAGAATCTATCAAAGAGAAATCTTCTTTTGTCCCTTCAACAGTTTTAG CTTTATTACTGTTCTTTTCTGAAGGAAGTTTTAAACTCTCTACTTCTTTTTCCTTTGCTT GTTTTTCATCTTTAACTTTCTGTGTATCTTCACTCCTTTTTGAGTGATCAAATTTCTTTT CAGTCTTTTCCTTCTTTTCTTTCTTTCTTGCTCCTTTCTCATGGCTGGCTGGCTTCTTTT CACCTTTGGCTGTTGATTTAATTTTNTGGCTCACTGGCTTTACTGTTTGACATCCTTATT TN
Restriction Sites	NotI-NotI
ACCN	AF528529
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF528529.1, AAM94279.1
RefSeq Size	2610 bp
RefSeq ORF	2610 bp
Locus ID	259282
Gene Summary	Component of the fork protection machinery required to protect stalled/damaged replication forks from uncontrolled DNA2-dependent resection. Acts by stabilizing RAD51 at stalled replication forks and protecting RAD51 nucleofilaments from the antirecombinogenic activities of FBH1 and BLM (PubMed:26166705). Does not regulate spindle orientation (PubMed:26166705).[UniProtKB/Swiss-Prot Function]

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