NUP160 (AK026236) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026236, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGCGGGAACCCTGGAACGGAGCTTCGTGGAGCTAAGCGGAGCTGAGCGCGAAAGGCCGAGGC ACTTTCGGGAATTCACAGTCTGCAGCATTGGGACTGCAAATGCCGTGGCTGGCGCCGTAAAATACAGTGA AAGCGCGGGAGGCTTTTACTACGTGGAGAGTGGCAAGTTGTTCTCCGTAACCAGAAACAGGTTCATTCAT TGGAAGACCTCTGGAGATACATTGGAGCTGATGGAGGAGTCACTGGACATAAATCTGTTGAATAATGCCA TTCGCCTAAAATTCCAAAATTGCAGTGTTTTACCTGGAGGGGTTTATGTCTCTGAGACTCAGAATCGTGT GATAATCTTGATGTTAACCAATCAAACAGTGCACAGGTTACTTTTACCACACCCCTCCCGGATGTATAGG AGTGTAAGTTGGCTAAGTGCAATATCTTTTATTTCCCAAATTACTCTGGGTGTCACAAATGTAGTGCTGG AGCGATGTCTTTTGGAATTGAAGGAAATTTGGATTCTCGTTATCCCTCACCAAGCATACTTTGATAGCTA CCGCTTAAAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026236 unedited GCTGCACCAATAGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCGCGGC GACGGCGGGAAGATGGCGGCGGCGGGAGCCCTGGAACGGAGCTTCGTGGAGCTAAGCGGA GCTGAGCGCGAAAGGCCGAGGCACTTTCGGGAATTCACAGTCTGCAGCATTGGGACTGCA AATGCCGTGGCTGGCGCCGTAAAATACAGTGAAAGCGCGGGAGGCTTTTACTACGTGGAG AGTGGCAAGTTGTTCTCCGTAACCAGAAACAGGTTCATTCATTGGAAGACCTCTGGAGAT ACATTGGAGCTGATGGAGGAGTCACTGGACATAAATCTGTTGAATAATGCCATTCGCCTA AAATTCCAAAATTGCAGTGTTTTACCTGGAGGGGTTTATGTCTCTGAGACTCAGAATCGT GTGATAATCTTGATGTTAACCAATCAAACAGTGCACAGGTTACTTTTACCACACCCCTCC CGGATGTATAGGAGTGAGTTGGTAGTTGACAGTCAGATGCAGTCAATATTCACTGACATT GGAAAAGTTGATTTCACAGATCCTTGCAACTATCAGTTAATTCCAGCAGTACCTGNAATA TCTCCTAATTCCACCGCCTCTACAGCCTGGCTCAGCAGTGATGGGGAGGCCCTGTTTGCC TTACCATGTGCTTCTGGGGGAATCTTTGTTCTTAAGCTACCTNCTTATGACATACCNTGT ATGGTGTCAGTCGTGGAACTGAAACAGAGNTCAGTAATGCAACGATTGCTTACAGGCTGG ATGCCAACAGCTATCAGGGGTGACCAGTCCCCTTCAGATCGTCCCCTCAGTCTTGCTGTT CATTGTGTGGAGCATGATGCCCTCATCTTTGCTTTGTGTCAGGATCATAAACTACCGATG TGGCCTTACAAGGAGCAAATGTGCCTAATGG
Restriction Sites	NotI-NotI
ACCN	AK026236
Insert Size	5000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026236.1, BAB15406.1
RefSeq Size	1327 bp
RefSeq ORF	573 bp
Locus ID	23279
Gene Summary	NUP160 is 1 of up to 60 proteins that make up the 120-MD nuclear pore complex, which mediates nucleoplasmic transport.[supplied by OMIM, Apr 2004]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.