RNase H2 subunit C (RNASEH2C) (AF312034) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGS3; AYP1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for AF312034 edited ATGGAGAGCGGCGACGAAGCGGCCATCGAGAGGCACCGCGTCCACTTGCGCTCCGCCACA TTGCGCGACGCCGTACCCGCCACACTGCATCTGCTGCCCTGCGAGGTTGCGGTGGACGGG CCCGCCCCGGTGGGGCGCTTCTTCACGCCCGCCATCCGCCAGGGCCCCGAGGGACTCGAA GTGTCGTTTCGGGGCCGCTGTCTACGGGGAGAGGAGGTGGCGGTGCCGCCTGGCCTCGTG GGATACGTGATGGTGACAGAAGAGAAGAAGGTGTCGATGGGGAAGCCAGACCCCTTGCGG GATTCCGGGACTGACGACCAAGAGGAGGAGCCGCTGGAGCGGGACTTCGACCGCTTCATT GGAGCCACTGCCAACTTCAGCCGCTTCACCCTGTGGGGTCTGGAGACCATCCCTGGCCCG GATGCCAAAGTGCGTGGGGCCTTAACTTGGCCCAGCCTTGCGGCAGCGATTCACGCACAG GTGCCCGAGGACTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF312034 unedited NGGGTCCCCAATAGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCTGGCGT CGCGTAGGAGGGAGGATGGAGAGCGGCGACGAAGCGGCCATCGAGAGGCACCGCGTCCAC TTGCGCTCCGCCACATTGCGCGACGCCGTACCCGCCACACTGCATCTGCTGCCCTGCGAG GTTGCGGTGGACGGGCCCGCCCCGGTGGGGCGCTTCTTCACGCCCGCCATCCGCCAGGGC CCCGAGGGACTCGAAGTGTCGTTTCGGGGCCGCTGTCTACGGGGAGAGGAGGTGGCGGTG CCGCCTGGCCTCGTGGGATACGTGATGGTGACAGAAGAGAAGAAGGTGTCGATGGGGAAG CCAGACCCCTTGCGGGATTCCGGGACTGACGACCAAGAGGAGGAGCCGCTGGAGCGGGAC TTCGACCGCTTCATTGGAGCCACTGCCAACTTCAGCCGCTTCACCCTGTGGGGTCTGGAG ACCATCCCTGGCCCGGATGCCAAAGTGCGTGGGGCCTTAACTTGGCCCAGCCTTGCGGCA GCGATTCACGCACAGGTGCCCGAGGACTGAGAACCAGAGCTTGAAATTCAAAGCTGCGAT CCCTTCACAGCAGTAAACCAGCTCTTTGGAACCGATTCCATCACCCCAATAAAGGAGCTC TTCACAGCACCTGTGAGTCTGGGCGCCTTANAGCCCCTAAGCCAGCATGGGCCCTTTCCC CCTCCCCACCAAGAAACTTGCATGGCTCTGGCATATAGAAACATTTATTGCCCGNGGCAA TANGGGTAAGAGAAGCCACGAAATTTTTGAGGCAGAACCCACCCCACCCCAACTTTTACT TTACAAAGGAATTNTTACAATCACAAGCCTCTGTACAGAGCTNCCCCATNCGNATCCCTT TNACTGTACCAG
Restriction Sites	NotI-NotI
ACCN	AF312034
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF312034.1, AAL87739.1
RefSeq Size	514 bp
RefSeq ORF	514 bp
Locus ID	84153
Protein Pathways	DNA replication
Gene Summary	This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008]

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