GANC (AK074037) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074037, the custom clone sequence may differ by one or more nucleotides AGAAACGCTAGTTTGGGCCTGAAAAATTCCAGGAGCAAGAGTCAAGATTTGTCACTCCATGAGAATCTGG AGGGGACTCCCTTCCCAGAAACTTGACGATGAAGTACTGGTTGTAATTTTAGAAAGACACCCAATCGGCT TTTTTAAAAGATCGCCCAGGGCCCTTGTCCTGAGAGCTGGGAGCTGGTCGGAGTGACAGAGAAGCCATGG AAGCAGCAGTGAAAGAGGAAATAAGTGTTGAAGATGAAGCTGTAGATAAAAACATTTTCAGAGACTGTAA CAAGATCGCATTTTACAGGCGTCAGAAACAGTGGCTTTCCAAGAAGTCCACCTATCGGGCATTATTGGAT TCAGTCACAACAGATGAAGACAGCACCAGGTTCCAAATCATCAATGAAGCAAGTAAGGTTCCTCTCCTGG CTGAAATTTATGGTATAGAAGGAAACATTTTCAGGCTTAAAATTAACGAAGAGACTCCTCTAAAACCCAG ATTTGAAGTTCCGGATGTCCTCACAAGCAAGCCAAGCACTGTAAGGCTGATTTCATGCTCTGGGGACACA GGCAGTCTGATATTGGCAGATGGAAAAGGAGACCTGAAGTGCCATATCACAGCAAACCCATTCAAGGTAG ACTTGGTGTCTGAAGAAGAGGTTGTGATTAGCATAAATTCCCTGGGCCAATTATACTTTGAGCATCTACA GATTCTTCACAAACAAAGAGCTGCTAAAGAAAATGAGGAGGAGACATCAGTGGACACCTCTCAGGAAAAT CAAGAAGATCTGGGCCTGTGGGAAGAGAAATTTGGAAAATTTGTGGATATCAAAGCTAATGGCCCTTCTT CTATTGGTTTGGATTTCTCCTTGCATGGATTTGAGCATCTTTATGGGATCCCACAACATGCAGAATCACA CCAACTTAAAAATACTGGTGATGGAGATGCTTACCGTCTTTATAACCTGGATGTCTATGGATACCAAATA TATGATAAAATGGGCATTTATGGTTCAGTACCTTATCTCCTGGCCCACAAACTGGGCAGAACTATAGGTA TTTTCTGGCTGAATGCCTCGGAAACACTGGTGGAGATCAATACAGAGCCTGCAGTAGAGTACACACTGAC CCAGATGGGCCCAGTTGCTGCTAAACAAAAGGTCAGATCTCGCACTCATGTGCACTGGATGTCAGAGAGT GGCATCATTGATGTTTTTCTGCTGACAGGACCTACACCTTCTGATGTCTTCAAACAGTACTCACACCTTA CAGGCACACAAGCCATGCCCCCTCTTTTCTCTTTGGGATACCACCAGTGCCGCTGGAACTATGAAGATGA GCAGGATGTAAAAGCAGTGGATGCAGGGTTTGATGAGCATGACATTCCTTATGATGCCATGTGGCTGGAC ATAGAGCACACTGAGGGCAAGAGGTACTTCACCTGGGACAAAAACAGATTCCCAAACCCCAAGAGGATGC AAGAGCTGCTCAGGAGCAAAAAGCGTAAGCTTGTGGTCATCAGTGATCCCCACATCAAGATTGATCCTGA CTACTCAGTATATGTGAAGGCCAAAGATCAGGGCTTCTTTGTGAAGAATCAGGAAGGGGAAGACTTTGAA GGGGTGTGTTGGCCAGGTCTCTCCTCTTACCTGGATTTCACCAATCCCAAGGTCAGAGAGTGGTATTCAA GTCTTTTTGCTTTCCCTGTTTATCAGGGATCTACGGACATCCTCTTCCTTTGGAATGACATGAATGAGCC TTCTGTCTTTAGAGGGCCAGAGCAAACCATGCAGAAGAATGCCATTCATCATGGCAATTGGGAGCACAGA GAGCTCCACAACATCTACGGTTTTTATCATCAAATGGCTACTGCAGAAGGACTGATAAAACGATCTAAAG GGAAGGAGAGACCCTTTGTTCTTACACGTTCTTTCTTTGCTGGATCACAAAAGTATGGTGCCGTGTGGAC AGGCGACAACACAGCAGAATGGAGCAACTTGAAAATTTCTATCCCAATGTTACTCACTCTCAGCATTACT GGGATCTCTTTTTGCGGAGCTGACATAGGCGGGTTCATTGGGAATCCAGAGACAGAGCTGCTAGTGCGTT GGTACCAGGCTGGAGCCTACCAGCCCTTCTTCCGTGGCCATGCCACCATGAACACCAAGCGACGAGAGCC CTGGCTCTTTGGGGAGGAACACACCCGACTCATCCGAGAAGCCATCAGAGAGCGCTATGGCCTCCTGCCA TATTGGTATTCTCTGTTCTACCATGCACACGTGGCTTCCCAACCTGTCATGAGGCCTCTGTGGGTAGAGT TCCCTGATGAACTAAAGACTTTTGATATGGAAGATGAATACATGCTGGGGAGTGCATTATTGGTTCATCC AGTCACAGAACCAAAAGCCACCACAGTTGATGTGTTTCTTCCAGGATCAAATGAGGTCTGGTATGACTAT AAGACATTTGCTCATTGGGAAGGAGGGTGTACTGTAAAGATCCCAGTAGCCTTGGACACTATTCCAGTGT TTCAGCGAGGTGGAAGTGTGATACCAATAAAGACAACTGTAGGAAAATCCACAGGCTGGATGACTGAATC CTCCTATGGACTCCGGGTTGCTCTAAGCACTAAGGGTTCTTCAGTGGGTGAGTTATATCTTGATGATGGC CATTCATTCCAATACCTCCACCAGAAGCAATTTTTGCACAGGAAGTTTTCATTCTGTTCCAGTGTTCTGA TCAATAGTTTTGCTGACCAGAGGGGTCATTATCCCAGCAAGTGTGTGGTGGAGAAGATCTTGGTCTTAGG CTTCAGGAAGGAGCCATCTTCTGTGACTACCCACTCATCTGATGGTAAAGATCAGCCTGTGGCTTTTACG TATTGTGCCAAAACATCCATCCTGAGCCTGGAGAAGCTCTCACTCAACATTGCCACTGACTGGGAGGTCC GCATCATATGACAAAGAACTGCCCCTGGTGATGTGAGCAGGGACCTGCCTGCCCCTTTCAACCTTTCCCC TCACCTTTTTTGAGATTTTTGCTGCAATCTGTTTGCCTTCCCTGAATCAAAATAATCTTTCATTCGTCAC CATTATACTAATGAACAATAGATTTCATGTTTCAAAATTTCAGATTTTACATGTTAAGATGTACTAACAA TATTCCTTGTATCAAACATCTCCTTTTCTCCCTGATACATAGCCCTGAGACATTTATAGCGTTCAGGAGT CTTCTATTGCTTCCATTCCTTCAGCAGGGCTGCGTGGGTCTGTTTTAACGTGGGCCAAGCCTACCTGGGC AGCCCATTTGCCAGGGCTTGCCTCAGGCCATGCAGCATTGGCGCTCTGGCTGCAGCAGCTGAGTTGCTCA AGGCCAGTGTCCAAGTGGACAGCAGCCTCTGGTACTCCCCCCAGTTATCTTCCACCCACATGGACTGGGC AGAGCAGCCCTCTTCTGTGTGCACTGCATACGCTGCAGCCGTGGGAGTTATTCTCCCCTAGAGATCGACT TGGCAGCACGAAGGATTCTTTTCTCTTTCATGCTTCTCAGGCTCAATAGTTTCTAATTAATCTTAAAATC CATGTCTTTTACATTGTTTTTTTAATTAAGTGCTGTTTACTAACCAAATAATATTTATAACATGAGTAAG CTATAATTAATAACAATGAAATAAATACCCATGTACCCACCACTGGACTTCAGAAGTAGAACTCATGACT GGGACTAGGATGAGGCAAGGGAGACCCTGGCCTTGGGCACAAAATGTAAGGGATGCCAAAAAAATACAGT AATCAAAGTAAGTAATATTTCAATCCAATATTTTTAAAAATCAGAATTAATGCAAAAAAAACCATGATGA ACAAAATATTAAAATTTAAAATAAAGACAGGATTAGTATTACTGAGTTTTCCTTTTGTCCCAGGCTCTAA TATGGCTTGGCATGGGGCAGAACATTACAACATACCAGTCGTGTCATGGTGCCCAAGGCTCCACAGACCT CAGTGGCTCCCTGCTGCCTGCCACAGCATCTGTTTTAGCAGCCTCGACTCCTCAGCACTCCTCAGCACAC ACCTCTTCTTATCAGGCTTCCTCCACTTAGCAACTTGCTAACGGCCACCTCTGTGCCTTCTGATCCCTGG GCGCCAATATCCTCCTGCCCTTACCATCCTTCCAGGCCCAACTTAAATCCCACTTTCCCATGAAGCCTAA CTGCGTGAACACCCCTACCCCCATACCCATTAGCAGTGATTTTGCCCTTCCCCGTAATGCTGTCCCACTT ATAACTGTGCTCTACTTAGCATTCTCAGGGATCATACCTTAATGTTTTCAGTATGTCTGCGTTCTCCTAC TAGATTGTATGTCCCTCAAGAGCATGTTCTGTTTCTCTTCTGTCTGACAGAGCACTATTATACCTGACTT TCAGTAACTGTTAGCTGTGATTAGTTAGCTGGTGGATTTAATTGATTAAAAAATTACGATTGAATGT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074037 unedited CCCAATTCCCCCGCCCGTTGACGCAATGGGCGGTAGGCGTGTACGGTGGNGAGGTCTATA TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG CCGCGAATTCGGCACGAGGGTCATCCTGTTGGAACCTGGACTCATTGCGTACACGCCCTC ACCGCCGCCCAGTTTCGGTTCCTAACAAAAGCACGTTCCTCATCAGCCACCCATAATCAA GACAAATTTGCCAAATAAATCATTGTAGAAACGCTAGTTTGGGCCTGAAAAATTCCAGGA GCAAGAGTCAAGAGTTGTCACTCCATGAGAATCTGGAGGGGACTCCCTTCCCAGAAACTT GACGATGAAGTACTGGTTGTAATTTTAGAAAGACACCCAATCGGCTTTTTTAAAAGAGCG CCCAGGGCCCTTGTCCTGAGAGCTGGGAGCTGGTCGGAGTGACAGAGAAGCCATGGAAGC AGCAGTGAAAGAGGAAATAAGTGTTGAAGATGAAGCTGTAGATAAAAACATTTTCAGAGA CTGTAACAAGATCGCATTTTACAGGCGTCAGAAACAGTGGCTTTCCAAGAAGTCCACCTA TCGGGCATTATTGGATTCAGTCACAACAGATGAAGACAGCACCAGGTTCCAAATCATCAA TGAAGCTAGTAAGGTTCCTCTCCTGGCTGAAAGTTATGGTATAGAAGGAAACATTTTCAG GCTTAAAATTAACGAAGAGACTCCTCTAAAACCCAGAGTTGACAGTCCTGGATGTCCTCA CAAGCTAGCCAAGCACTTGTAGGGCTGATTTCATGCTCNTGGGACACAGGCAGTCTGATA TTGGCAGATGGAAAAGGAGACCTGAAGTGCCATATCACAGCAAACCATTCANGTAGACTT GGTGTCTGAGAACGAGTTGTGATAGCATACATTCCTGGCCATTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK074037 unedited TTTCCAGAAATACTATGNACCGCGCCGCTATCTAGATCGAGTTTTTTTTTTTTTTTTTTA CATTCAATCGTAATTTTTTAATCAATTAAATCCACCAGCTAACTAATCACAGCTAACAGT TACTGAAAGTCAGGTATAATAGTGCTCTGTCAGACAGAAGAGAAACAGAACATGCTCTTG AGGGACATACAATCTAGTAGGAGAACGCAGACATACTGAAAACATTAAGGTATGATCCCT GAGAATGCTAAGTAGAGCACAGTTATAAGTGGGACAGCATTACGGGGAAGGGCAAAATCA CTGCTAATGGGTATGGGGGTAGGGGTGTTCACGCAGTTAGGCTTCATGGGAAAGTGGGAT TTAAGTTGGGCCTGGAAGGATGGTAAGGGCAGGAGGATATTGGCGCCCAGGGATCAGAAG GCACAGAGGTGGCCGTTAGCAAGTTGCTAAGTGGAGGAAGCCTGATAAGAAAAGGTGTGT GCTGAGGAGTGCTGAAGAGTCCAGGCTGCTAAAACAGATGCTGTGGCAGGCAGCAGGGAG CCACTGAAGTCTGTGGAGCCTTGGGCACCATGACACGACTGGGATGTTGGTATGTTCTGC CCCATGCCAAGCCCTATTAGAACCTGGGACAAAAGGGAAACTCACGTATACTAATCCTGG CTTTATTTTAAATTTTAAAAATTTGGTTCTCAAGGGTTTTTTTGCATTAAATCCTGATTT TAAAAAAATTGGATTGGAAAATTACTTACCTTGGATACCGGGATTTTTTTTGGGCAACCC CTTAATTTTTGTGCCCCAAGGCCAGGGTCTCCCCTTGGCCTATCCCTAGCCCCAGTCATG GATTTTTACTTTTGGAAGTCCCGTGGGGGG
Restriction Sites	NotI-NotI
ACCN	AK074037
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK074037.1, BAB84863.2
RefSeq Size	4477 bp
RefSeq ORF	2745 bp
Locus ID	2595
Domains	Glyco_hydro_31
Protein Families	Druggable Genome
Protein Pathways	Galactose metabolism, Metabolic pathways, Starch and sucrose metabolism
Gene Summary	Glycosyl hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. This gene encodes a member of glycosyl hydrolases family 31. This enzyme hydrolyses terminal, non-reducing 1,4-linked alpha-D-glucose residues and releases alpha-D-glucose. This is a key enzyme in glycogen metabolism and its gene localizes to a chromosomal region (15q15) that is associated with susceptibility to diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.