SLC25A42 (AF521885) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC104674 sequence for AF521885 edited (data generated by NextGen Sequencing) ATGCTGCTGATTCTAGTGACCCCTGTCCCCACCAGGCTCAGAGCCAGACTGCGCCTGGAC CTTCTTGTTCTGACTCCACGTGCCTGCCCGGCCTCCAGGGTGCGGGGGCGCCTTTCTTGC AGGCGGACCCTGCCCAGGATGGGGCCAGCCTCGTGCTCAGCTTTGGCCACAAATGCAGCC CCTGGCCCACCCCACCCTGCCGGCCCTGCCTTCTCCAGTATTTCCCACATGGCCACGACT CCTCAGTCACTAGAGCCTCCTGCTGGGAACAGTGTCCCCCAGAGCCTCATGTCTATCCTA GACCCTGCAAGCAGCTGGGTCCCCAAGAGTGCATCTCCCCCTAGAGTTGCCTGCCCATGC CCACCTGCTTTGTAA Clone variation with respect to AF521885.1 50 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF521885 unedited GGCGTGTTCAAAATTTGTAATACGACTCACTATAGGCGGCCGCGAAATTCGCACCAGGGG CCTCTACAAAGGCTTGAGCATGAACTGGGTCAAGGGTCCCATCGCCGTGGGCATCAGCTT CACCACCTTCGACCTCATGCAGATCCTGCTGCGGCACCTGCAGAGCTAGGGGACCCTGAG CTGCTCTCAGGACGGTGGACCGGTGACCCCTTTGTATTCTGGGCCCATGGAACGGTGGGG GGGTGCGCTTGATTCTACTTCAGGAGGCACATGGGGCGCTTTATGGAACGAGCAGGTGGG CCTGAGGGGCCTGGGCTCAGAGTCCACGTCCAAACGCAAAGCTGGCAGCCCTGGAAGTGC AGTGTTGGGGCGATGGTGTGGGGGGTCCCGCAGCTCCCCTCTTCCTTCCTCTGCAGACGC TGGCGCTGTCTGCCGGAGGTGTTGCCCAAAAGGCCCTAGTGGGGCGTGGTCAGCTCCACC TCCTGATCCTGTGTGTCCTCCGACATGCTGCTGATTCTAGTGACCCCTGTCCCCACCAGG CTCAGAGCCAGACTGCGCCTGGACCTTCTTGTTCTGACTCCACGTGCCTGCCCGGCCTCC AGGGTGCGGGGGCGCCTTTCTTGCAGGCGGACCCTGCCCAGGATGGGGCCAGCCTCGTGC TCAGCTNTGGCCACAAATGCAGCCCCTGGCCCACCCCACCCTGCCGGCCCTGCCTTCTCC AGTATTTCCCACATGGCCACGACTCCTCAGTCACTAGAGCCTNCTGCTGGGAACAGTGTN CCCCGAGCCTCATGTCTATCCTAGACCCTGCAGCAGCTGGGTCCCCAAGATGCATCTCCN NCTAAAGTGCCTGCCATGCCCACTGCTTTGTACCTTCCAGAGATCATGCTTGCTTGCACA CAGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF521885 unedited ATCGCTGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTCAT TTAAGTTTTTATTAAATATACTCTTTTGGGGCAAATGTTTAAAATATATAAACCTTAGGA TATAAACAGTGTGTTCATGGGGTCAAAATATAACTCCAGTACCAAGACCAGTGACCAGCA TGGAGGCAAGTGACCGCAGAGCCTGAGGGCCTGGCTGTGTGCAAGGGTGGGGGACAGCAC TGTCCCCAACTGCTGCCGGGTCCTACTTATCTAGGAGCCATCTTCGGGGAGACACACTGG GGTCAGCAGGGACTGGGGGACCCTCCCACCTACCCCTTGTCTATACTGTCTAGCCTGTGG CCCCGAATGCACTCATTTGGTCTCACCCACCTGGGGCAGACCCCCAATTTCTACTGATGT TGCATGCTTGTGGGAATTGGGTGCCCTCCCATCTCCCTGTGAGGGTTGCCCTGGCTCTAC TGCCAAGTTTAAATGCTGGGTAAGGGATACCCGCACAGTGGGACAGCCGCTCACAGGCAT CACAGGCTGACTGGGCCTATGGGGACAAGATCACGGATGGCTGGTGGCCACATGCTAAGA ATGCAGACATAGCGGTCTGCACACCTGCTTGTCCCAAGCAAGAACCCGAGGAGGACAGAA TGAATGCAAATGAAGTCACGACTGGGCATGAACAGCTCACATGCTTGCAACAGTGTGTGC TGATCCAGCTGTGGAAAGGAACATCATGGGCACGCTGACCCTGTCACCAGCCAGACAGTC AGGGTTCCATGTGAATAGATCATGAACCCGGGTCAGCCCGTTTGAATACGAATCNGATCC TTCAAAAAAAGGAAGCCCGAACCCACGCTGTCCTTCGATATGGGAGATAATGAAACCTGG GATAACTGCCAGGCCCTGGGGGGTCCTCTGAGAACCAGTAGCTGGGGGTTCTCCTGGGCA CTCACATTACCCTACTTCCACCCTAGATTACTC
Restriction Sites	NotI-NotI
ACCN	AF521885
Insert Size	5500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF521885.1, AAM81327.1
RefSeq Size	2338 bp
RefSeq ORF	375 bp
Locus ID	284439
Protein Families	Druggable Genome
Gene Summary	This gene encodes a solute carrier family 25 protein. Solute carrier family 25 proteins are localized to mitochondria and play critical roles in the transport of molecules across the inner mitochondrial membrane. The encoded protein is a mitochondrial transporter for coenzyme A (CoA) and adenosine 3',5'-diphosphate. [provided by RefSeq, Feb 2012]

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