SH3BP5L (AK056345) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK056345, the custom clone sequence may differ by one or more nucleotides ATGCACAACGCTGCTCGAGAAATGGTGTTTGTGGCTGAGCAGGGCGTCATGGCTGACAAGAACCGACTGG ACCCCACGTGGCAGGAGATGCTGAACCATGCTACCTGCAAGGTGAATGAGGCGGAGGAAGAGCGGCTTCG AGGTGAGCGGGAGCACCAGCGAGTGACTCGGCTGTGCCAACAGGCTGAGGCTCGGGTCCAAGCCCTGCAG AAGACCCTCCGGAGGGCCATCGGCAAGAGCCGCCCCTACTTTGAGCTCAAGGCCCAGTTCAGCCAGATCC TGGAGGAGCACAAGGCCAAGGTGACAGAACTGGAGCAGCAGGTAGCTCAGGCCAAGACGCGCTACTCCGT GGCCCTTCGTAACCTGGAGCAGATCAGCGAGCAGATTCACGCACGGCGCCGCGGGGGTCTGCCTCCCCAC CCCCTGGGCCCTCGGCGCTCCTCCCCCGTGGGGGCCGAGGCAGGACCCGAGGACATGGAGGACGGAGACA GCGGGATTGAGGGGGCCGAGGGTGCGGGGCTGGAGGAGGGCAGCAGCCTGGGGCCCGGCCCCGCCCCCGA CACCGATACCCTGAGTCTGCTGAGCCTGCGCACGGTGGCTTCAGACCTGCAGAAGTGCGACTCCGTGGAG CACTTGCGAGGCCTCTCGGACCACGTCAGTCTGGACGGCCAAGAGCTGGGAACGCGGAGTGGAGGGCGCC GGGGCAGCGACGGCGGAGCCCGTGGGGGTCGGCACCAGCGCAGCGTCAGCCTGTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK056345 unedited NGGTAACCGTACAAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACCAGN AGAGGAGGAAGAACTGGCTCCTAGAATACAGGAGGAGTTGGAGCACCTGAACCAGGCCAG CGAGGAGATCAACCAGGTGGAACTACAGCTGGATGAGGCCAGGACCACCTATCGGAGGAT CCTACAGGAGTCGGCGAGGAAACTGAATACACAGGGTTCCCACTTGGGGAGCTGCATCGA GAAAGCCCGGCCCTACTATGAGGCTCGGCGGCTGGCTAAGGAGGCTCAGCAGGAGACACA GAAGGCAGCGCTGCGGTACGAGCGGGCCGTAAGCATGCACAACGCTGCTCGAGAAATGGT GTTTGTGGCTGAGCAGGGCGTCATGGCTGACAAGAACCGACTGGACCCCACGTGGCAGGA GATGCTGAACCATGCTACCTGCAAGGTGAATGAGGCGGAGGAAGAGCGGCTTCGAGGTGA GCGGGAGCACCAGCGAGTGACTCGGCTGTGCCAACAGGCTGAGGCTCGGGTCCAAGCCCT GCAGAAGACCCTCCGGAGGGCCATCGGCAAGAGCCGCCCCTACTTTGAGCTCAAGGCCCA GTTCAGCCAGATCCTGGAGGAGCACAAGGCCAAGGTGACAGAACTGGAGCAGCAGGTAGC TCAGGCCAAGACGCGCTACTCCGTGGCCCTTCGTAACCTGGAGCAGATCAGCGAGCAGAT TCACGCACGGCGCCCGCGGGGGTCTGCCTCCCCACCCCCTGGGCCCTCGGCGCTCCTCCC CCGTGGGGGCCGAGGCAGGACCCCGAGACATGGNAGACGGAGACAGCGGGATTGAGGGGC CCCAGGGTGCCGGGCTGGNAAGAGGGCAACAACCTGGGGGCCCGGCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK056345 unedited NNNNCGCCCCTACTATGNACCGCGGCACGCAATCTAAGATCGATTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTAACTGTTACAAAAACTACTTTATTTAAATAAAACCAGGG AAAGACCCTTTCCCCCTTACACACCAGCACATGCACACACGCACACCCACACCCTCCCTC CCTTTCGGCTGTCTGCCCATGGCCACCTCAAACCCCGAGGTTCTCATTGGGACCAACCTC CCTCAACCTGTAAACCCCCGTGCAAAGTCCAGTTGCCCACGCATCCCGGTTGCTGGCAAA ACATGGGCCTAAAAAAAAACACTCCATCTTCCTGGGGGGGGTCAAAAGGAAAGACTGGTT CTCCCAAGGCACCCAAATCTGGGGGGGCTTCCCTACAAACTGGGAGCACTGCCAGGCAGC TGGAGCTGCTCCCTGCGGAGGGCAAAACTTTTCACCCCAAGCCAACCCCTCCCTGTAACA AAGGAAGTAAAAAGGTTGGCAGCCAAGCTTCAGTGTGCCCCGAGGGATCCTGCAGTAGGT ATTTTCAGTTTGGGGAAAAACAAGGGAGAGCAATTGAGCACCAAATTCCCTAACCCCGTC CTGCAGGAAGGTGCTGAGTCAGTTCAACATCCTCTACCCAGAAAAAGTAAACACAACTGT GCAGCAAGAGTGAGGGTGGGAATGACAGGGGCCTAAGCCTTCAGGACCTCATGACTTCAC CCCCAACATGGGCCCTCTTAAGGACCCACTAGCACCTTGGCAATGTTGAGGACCCAGCCC AGCGGAAACACCATACCCACAGGAGCCAGTCTGGAGTTGGGGGATGCTCCCTATGTTGAC AAGGGGGGTTGGGTTT
Restriction Sites	NotI-NotI
ACCN	AK056345
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK056345.1, BAB71158.1
RefSeq Size	2367 bp
RefSeq ORF	2367 bp
Locus ID	80851
Domains	SH3BP5
Gene Summary	Functions as guanine nucleotide exchange factor (GEF) for RAB11A.[UniProtKB/Swiss-Prot Function]

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