MRPL45 (NM_032351) Human Untagged Clone
CAT＃: SC105391
MRPL45 (untagged)-Human mitochondrial ribosomal protein L45 (MRPL45), nuclear gene encoding mitochondrial protein
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | L45mt; MRP-L45 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_032351, the custom clone sequence may differ by one or more nucleotides
ATGGCAGCCCCCATACCTCAAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTTCGGCAGCCAG TTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGTTTCACACCTCCTATTTATCA ACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCCCGGAAAGCAGGATTGGTTATTCCTCCAGAA AAATCGGACCGTTCCATACATCTGGCCTGTACAGCTGGTATATTTGATGCCTATGTTCCTCCTGAGGGTG ATGCACGCATATCATCTCTTTCAAAGGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGC ATCACAAGTGTCAATCCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAA GCTAAGGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTTCATACCTTGG TAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACCGTCCGCTGGAGCTTTGTGGA ATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCAAGTATGATGAACCAGGGCAACGTGTACGGC CAGATCACCGTACGCATGCACACCCGGCAGACTCTGGCCATCTATGACCGGTTTGGCCGGTTGATGTATG GACAGGAAGATGTACCCAAGGATGTCCTGGAGTATGTTGTATTCGAAAAGCAGTTGACAAACCCCTATGG AAGCTGGAGAATGCATACCAAGATCGTTCCCCCATGGGCACCCCCTAAGCAGCCCATCCTTAAGACGGTG ATGATCCCTGGCCCTCAGCTGAAACCAGAAGAAGAATATGAAGAGGCACAAGGAGAGGCCCAGAAGCCTC AGCTAGCCTGA >OriGene 5' read for NM_032351 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGGAACAAGATGG CAGCCCCCATACCTCAGGGTTCTCTTGTTTATCGAGGTTTTTGGGCTGGTGGTTTCGGCA GCCAGTTCTGGTGACTCAGTCCGCAGCTATAGTTCCAGTAAGAACTAAAAAACGTTTCAC ACCTCCTATTTATCAACCTAAATTTAAAACAGAAAAGGAGTTTATGCAACATGCCCGGAA AGCAGGATTGGTTATTCCTCCAGAAAAATCGGACCGTTCCATACATCTGGCCTGTACAGC TGGTATATTTGATGCCTATGTTCCTCCTGAGGGTGATGCACGCATATCATCTCTTTCAAA GGAGGGACTGATAGAGAGAACTGAACGAATGAAGAAGACTATGGCATCACAAGTGTCAAT CCGGAGGATAAAAGACTATGATGCCAACTTTAAAATAAAGGACTTCCCTGAAAAAGCTAA GGATATCTTTATTGAAGCTCACCTTTGTCTAAATAACTCAGACCATGACCGACTTCATAC CTTGGTAACTGAACACTGTTTTCCAGACATGACTTGGGACATCAAATATAAGACCGTCCG CTGGAGCTNTGTGGAATCTTTAGAGCCCTCTCATGTTGTTCAAGTTCGCTGTTCAAGTAT GATGAACCAGGGGCAACGTGTACGGCCAGATCACCGTACGCATGCACACCCGGCAGACTC TGGGCATCTATGACCGGGTTGGCCGGNTGATGTATGGACAGGAAAATGTACCCAGGNATG CCTGAAGTATTGTTGATCGAAAAGCAGTGACCAAACCCTATGGAGCTGGAGAAGCTACAA GAACGTTNCCATGGGGACCCCTAGCAGCCATCTTAAGACGGGAAGATCCCTGGCCCTANC TGAACCAGAGAGATTTGAAGAGC >OriGene 3' read for NM_032351 unedited
GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATTATTTCTTTG GATTTATATTTTTCCATAAAATGCAAATGCTGATTCATCAGTGAGTCAGTATATGAAAAA GGGCCTCTTAAATGTCTTATAAACACTAATTATTCTTCCCCAGTCTTCATTTCCTTAAAG TCACATCGCTCACAAGTAGGCTCATCTTCCACTTCTGCCATCTGAAGGCTGGTCCATGCC CAGCCTGAACCAGGGGAAATGTGCAGAACTCACCAAAATTTTTCCAACACCCTGACAACA TTTCATTTCAAACTCTGATCCCTGCCCTGTGATTACAAAGAGGATGCTGCTGGTTGTCTC TCACAGTCCCTGCTGTGGGAAAAACTGATATCCAATGTTCTCTGAAACATACTGTCTTTC ATCTAGACTCAGAAGCTAGACATAAAATTTAAAAAAGAAGAGTGTCCATGGCCATGTTAT ACCTGCCACCTGCTAGGGCCCAGTCATCAGTCATGGTTGCTGATGATGAGACTGCTGAAA AGACCTGAGCAGGATGGGAGAGAACAAAGGTAGTTCTTTTTATAGCATGAGGGGAATGGG AGACTTCAAAGCTTCCAGCAGCCTCATCACCCAGGCTTCACCCTAGAAGTCATTNTTGTC ATCAGGCTAGCTGAGGCTTCTGGGCCTCTCCTTGTGCCTCTTCATATTCTTCTTCTGGTT TCAGCTGAGGGCCAGGGATCATCACCGTCTTAAGATGGGCTNGCTAGGGGGNCCCATGGN GGAACGATCTNGTATGCATTCTCCAGCTTCATAGGGGTTGTCAACTGCTTTCGAAACACA TACTCAGGAATNCTTGGTACATTTNCTGTCCTACATAACCGGCAAACCGTATANATGCCA NATCTGCCGGTGTGCATGCCTCGGGATCTGCCGACACGTGGCCTGGTCATCTACTGAACA GGACTTGGACACTGAAAGCTTTAAAATCCCAAGCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032351 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032351.3, NP_115727.3 |
| RefSeq Size | 1713 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 84311 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. [provided by RefSeq, May 2013] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218954 | MRPL45 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L45 (MRPL45), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
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| RC218954L3 | Lenti-ORF clone of MRPL45 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L45 (MRPL45), nuclear gene encoding mitochondrial protein |
CNY 5890.00 |
|
| RC218954L4 | Lenti-ORF clone of MRPL45 (mGFP-tagged)-Human mitochondrial ribosomal protein L45 (MRPL45), nuclear gene encoding mitochondrial protein |
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|
| RG218954 | MRPL45 (tGFP-tagged) - Human mitochondrial ribosomal protein L45 (MRPL45), nuclear gene encoding mitochondrial protein |
CNY 4370.00 |
