CEECAM1 (CERCAM) (BD131097) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD131097, the custom clone sequence may differ by one or more nucleotides GAGCTCCGGGGGCCGCTGCAGCCGCCCAAGCGCCCGCCATGCGCGCTGCCCGCGCCGCGCCGCTGCTCCA GCTGCTGCTCCTGCTGGGGCCGTGGCTGGAGGCTGCGGGCGTTGCGGAGTCGCCGCTGCCCGCCGTGGTC CTTGCCATCCTGGCCCGCAATGCCGAACACTCGCTGCCCCACTACCTGGGCGCTCTGGAGCGGCTGGACT ACCCCCGGGCCAGGATGGNCCTCTGGTGTGCCACGGACCACAATGTGGACAACACCACAGAGATGCTGCA GGAGTGGCTGGCGGCTGTGGGCGATGACTATGCTGCTGTGGTCTGGAGGCCTGAGGGCGAGCCCAGGTTC TACCCAGATGAAGAGGGTCCCAAGCACTGGACCAAAGAAAGGCACCAGTTTCTGATGGAGCTGAAGCAGG AAGCCCTCACCTTTGCCAGGAACTGGGGGGCCGACTATATCCTGTTTGCAGACACAGACAACATTCTGAC CAACAATCAGACTCTGCGGCTTCTCATGGGGCAGGGGCTTCCAGTGGTGGCCCCAATGCTGGACTCCCAG ACCTACTACTCCAACTTCTGGTGTGGGATCACCCCCCAGGGCTACTACCGCCGCACAGCCGAGTACTTCC CCACCAAGAACCGCCAGCGCCGGGGCTGCTTCCGTGTCCCCATGGTCCACTCCACCTTCCTTGCATCCCT GCGGGCTGAAGGGGCAGACCAGCTTGCTTTCTACCCGCCACATCCCAACTACACTTGGCCTTTCGACGAC ATCATCGTCTTCGCCTATGCCTGCCAGGCTGCTGGGGTCTCCGTCCACGTGTGCAATGAGCACCGTTATG GGTACATGAATGTGCCGGTGAAATCCCACCAGGGGCTGGAAGACGAGAGGGTCAACTTCATCCACCTGAT CTTAGAAGCACTAGTGGACGGCCCCCGCATGCAGGCCTCAGCTCATGTGACTCGGCCCTCTAAGAGGCCC AGCAAGATAGGGTTTGACGAGGTCTTTGTCATCAGCCTGGCTCGCAGGCCTGACCGTCGGGAACGCATGC TCGCCTCGCTCTGGGAGATGGAGATCTCTGGGAGGGTGGTGGACGCTGTGGATGGCTGGATGCTCAACAG CAGTGCCATCAGGAACCTCGGCGTAGACCTGCTCCCGGGCTACCAGGACCCTTACTCGGGCCGCACTCTG ACCAAGGGCGAGGTGGGCTGCTTCCTCAGCCATTACTCCATCTGGGAAGAGGTGGTTGCCAGGGGCCTGG CCCGGGTCCTGGTGTTTGAGGATGACGTGCGCTTTGAGAGCAACTTCAGGGGGCGGCTGGAGCGGCTGAT GGAGGATGTGGAGGCAGAGAAACTGTCTTGGGACCTGATCTACCTCGGACGGAARCAGGTGAACCCTGAG AAGGAGACGGCCGTGGAGGGGCTGCCGGGCCTGGTGGTGGCTGGGTACTCCTACTGGACGCTGGCCTATG CCCTGCGTCTGGCGGGTGCCCGCAAGCTGCTGGCCTCACAGCCTCTGCGCCGCATGCTGCCCGTGGACGA GTTCCTGCCCATCATGTTCGACCAGCACCCCAACGAGCAGTACAAGGCACACTTCTGGCCACGGGACCTG GTGGCCTTCTCCGCCCAGCCCCTGCTCGCTGCCCCTACCCACTATGCCGGGGACGCCGAGTGGCTCAGTG ACACGGAGACATCCTCTCCATGGGATGATGACAGCGGCCGCCTCATCAGCTGGAGCGGCTCCCAAAAGAC CCTGCGCANCCCCGCCTGGACCTGACTGGCAGCAGCGGGCACAGCTCCAACCCCAGCCCCGAGATGAGCT CTAGGTCCAGGTGATGACTGCAAAGCAKKGTCCAGGAGCAGGCCACTACTGCCCAGAGAGCAGAGGAGGA GGTTGTTGGCAGGGACTNCAGATCCTGTCAGACCTGGCCACCACCTTGGGCATGGCCACTCTGCCCTCTG GACCTGTCTTTCATCGGGAGAAACCACTCAGAGATGGATCCCATTCCCTAAAGGTCTCACAGCAAAGGAG CAGGACTCCCAGGCCCCTGTACCCTGCCTGGCCTGATTCAGGGCCTTGTGGCCCCCAGCTTCTGTTTCAA GCTGGGCAGACCCCAGGATCCCTTCCCTCCCTAAGGACTCAGCTGAGGGGCCCCTCTGCCCCCTTCTACC TCCACCTCAGCACCCTCCCCCAGCTTGATGTTTGGGTCTCCCCAGCACCCTCCTCCCTGGCCGGTGCAAA GTACAGGGAGGTAAAGCAGGACCCTTGCAGACMTGTTGCCCAGCACACAGTAGGCCCTCAATAAAAGCCA TTTGCACTTTAAATATAAAAAAAAAAAAAAAAAAAAAAAAATA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD131097 unedited NTGTCAACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTGCAGCC GCCCAAGCGCCCGCCATGCGCGCTGCCCGCGCCGCGCCGCTGCTCCAGCTGCTGCTCCTG CTGGGGCCGTGGCTGGAGGCTGCGGGCGTTGCGGAGTCGCCGCTGCCCGCCGTGGTCCTT GCCATCCTGGCCCGCAATGCCGAACACTCGCTGCCCCACTACCTGGGCGCTCTGGAGCGG CTGGACTACCCCCGGGCCAGGATGGCCCTCTGGTGTGCCACGGACCACAATGTGGACAAC ACCACAGAGATGCTGCAGGAGTGGCTGGCGGCTGTGGGCGATGACTATGCTGCTGTGGTC TGGAGGCCTGAGGGCGAGCCCAGGTTCTACCCAGATGAAGAGGGTCCCAAGCACTGGACC AAAGAAAGGCACCAGTTTCTGATGGAGCTGAAGCAGGAAGCCCTCACCTTTGCCAGGAAC TGGGGGGCCGACTATATCCTGTTTGCAGACACAGACAACATTCTGACCAACAATCAGACT CTGCGGCTTCTCATGGGGCAGGGGCTTCCAGTGGTGGCCCCAATGCTGGACTCCCAGACC TACTACTCCAACTTCTGGTGTGGGATCACCCCCCAGGGCTACTACCGCCGCACAGCCGAG TACTTCCCCACCAAGAACCGCCAGCGCCGGGGCTGCTTCCGTGTCCCCATGGTCCACTCC ACCTTCCTTGCATCCCTGCGGGCTGANAGGGCAGACCAGCTTGCTNTCTACCCGCCACAT CCCAACTACACTTGGGCCTTTCGACGACATCATCGTCTTCGCCTATGCCTGCCANGCTGC TGGGGTCTCCGTCCACGTGTGCAATGAGCACCGTTATGGGTACATGAATGTGCCNNGTGA AAATCCACCACNNGGCTGAAGACGAGAGGGTCAACTCCATNCACTGATCTTAAAGCACTA C 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD131097 unedited NACGTNACACTATGACCGCGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTTTACA TATATATATATATATATATATATATACATATATATACATACATATATATATTTAAAGTGC AAATGGCTTTTATTGAGGGCCTACTGTGTGCTGGGCAACATGTCTACAAGGGTCCTGCTT TACCTCCCTGTACTTTGCACCGGCCAGGGAGGAGGGTGCTGGGGAGACCCAAACATCAAG CTGGGGGAGGGTGCTGAGGTGGAGGTAGAAGGGGGCAGAGGGGCCCCTCAGCTGAGTCCT TAGGGAGGGAAGGGATCCTGGGGTCTGCCCAGCTTGAAACAGAAGCTGGGGGCCACAAGG CCCTGAATCATGCCAGGCAGGGTACAGGGGCCTGGGAGTCCTGCTCCTTTGCTGTGAGAC CTTTAGGGAATGGGATCCATCTCTGAGTGGTTTCTCCCGATGAAAGACAGGTCCAGAGGG CAGAGTGGCCATGCCCAAGGTGGTGGCCAGGTCTGACAGGATCTGCAGTCCCTGCCAACA ACCTCCTCCTCTGCTCTCTGGGCAGTAGTGGCCTGCTCCTGGACACTGCTTTGCAGTCAT CACCTGGACCTAGAGCTCATCTCGGGGCTGGGGTTGGAGGCTGTGCCCGCTGCTGCCAGT CAAGTCCCAGGCGGGGCTGCGCAAGGTCTTTTGGGACCCGCTTCAGCTGATGAAGCCGCC GCTGTCATCATCCCATGGAGAGGATGTCTCCGTGTCACTGAGCCATTCGGCGTCCCCGGC TATTGGGTAAGGGCATCCATCCAGGGCCTGGGCGGAGAAAGCCACCCAGTCCCCGTGCCA GGATTGTGCCCTT
Restriction Sites	NotI-NotI
ACCN	BD131097
Insert Size	2450 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD131097.1
RefSeq Size	2353 bp
RefSeq ORF	2353 bp
Locus ID	51148
Domains	Glyco_transf_25
Gene Summary	Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.