KIAA1731 (CEP295) (AL833615) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL833615, the custom clone sequence may differ by one or more nucleotides GAAGATTTTAAAAATTCAACATACTTATAGATGGTTTCTCTTTAACTGTTTTGATTTGAGAATTTTCAGA GACATGAATTTTATTCCTTATTTCTTTCTGCCTCTGGTGGGATCTCTCCATAAATGATTTTTTCCTCTTT ATAAATGCTTCTTGTAAGCTCCCTGGTACAGGTGTAAGCCTGCGAGGGGTTTCTGCTGGGGGAAAAGAAA TTTATGACAGAAATAGGCAAAGTATCTACTTTCCATTAGAAGTGTGGAGGCCGTCCAAGGCTTACCTGTA GATGCTGTCCTTGGTTTTTCTATGGAAAGTTCTGATACAGCTGGATAATCTGAGGCTTCTGTTGCTGATA CAAGAGGCAGAAACACACTCACCTAATGGCAAGAAAGTTGCATGTTTTACTCTTTTTAAACGCTTTAAGC GGGTCTTGCTCTGCTGTCAGATTAGAGTGCAGCGGCATGATCACACCTCACTGCAGCCTCAATCTTAGCT GGGTCTACAGAGACCCGCCACCATGCCTGATTTTTGTATTTTTTGTAGGGATTGGGTTTTGCCTTGTTGC CCAGGCTGGTCTTGAACTGGGCTTAGGCTCCACTGCCCTCACACCTCTAGGCCTCTGTTTAGATAGCTTT ATTCTTATTGCCTCCCTACTCCCCTAGTAAAATTACCAGTTGTTCTGAAGTCATTTTTCAATATCTGGGA ATAAGTTTAGGTAAAACTTCCAACTTGGCACTATAACACTAGGTGTATAAGATTAGTTCTGCCAGTCTTG GAGACAGGCATAAATTGGAGTGGGGATTAAAAGAAAATGCACAATGGTTGACAGAATAGTGAAGGTTTAA GACAAATTGAGAGCTGGTGGGGGTGTCGGGTTAGTTAAATGCATAGCATAATTCCCTCCAATGAGTACCT TCTGATAAGTTGTAAGAGACAAATCAACTGGTTTGCTTGTAATAGAATAAAATAGAAGGAAAGCTTAAAT TTACCTGAAAGAAGCATTTTGCTTCATTCTCGCTCTTCTCTTCTAGGGTTAAATTTGCAAAATCCATTTC AGCAATACTGGTATCAGTGGTGCTTATTAAAGTAAGTTCTGGTTCTTCCATTATACCATGGCCAGTTTCT GTTTCCTGTGGACCACAATTAAAAGGAAAACCATGTCAAAATAGCCTTAAAAGACTTAAACTAATCGTTT TGTATATAATGCACATTTTTTGCAAGTGTATATAAATGTGAAAATGGAGGAAGAAAAGCAATGATTGGCA AAGACCAACAGATCAGATTTCCTTAGGCTTCCATAACTCCAGCATTCAGTTGTAGAAATACTGGAGCCTA CTTTGGGGAGGGGATGAAATAGTGTGACAATGGACTGAAAGGTATCTACCACAATGATTACAAGCCTAAA TGCTTGTAGTGTTGGGCAAAAAATATAAATGCAGGTTGGCTGTGCCGAATTGTATCCTTTAAAGGGAGCA GGGGTCGACTGGGCTGCTACCAAATGTGGGCCACTGACTGCTAACAGATAATGTTTCAAAAGTATCTAAA CACAACAAGAACAAAAGCAGCCACATAAAATGTGGTTTAGTTGTAATTCTTATTTTCAATCTGTTATGTT TATTTGTGGAAGGATAAACTGCACATACTGCTAGATGAAAACCAGTCACCAGTTGACTGGTGTTGAAAGC AAGCAGATTATAAGTTGCTTAATATGAGGAGCAGGTTTCCAATACTTATTCCAATAATGGATGAATTCAT TGCACTGGAATGAGGCTCAACACTGAAAGTGCTGAAGCACAGGCAGAACCACCATTTTTCAGGGAAGTTG GAGAAAGGATATCTGAATTGGGGGAAAAAGGATCTTTTGTAAAATACTCTCAGAAGACAGTTTTCAAATT TTTAAGTTTCTGCATTATGCTGGATATTTCTAGTGATCACAAGCTGGATAGCAATATTAACAAGCAGTTT TGCTTGTATTTTGTTTCAGGTTTTTCCTGCCAGGCATTGTGGTGTGTCCAAACTACTTCGGAAGTTGAGG CAGGAGGATCCCTTGATCCTAGGAGTTTGAGTTCAGCCTGGGTAGCATAGTGGGAACTTCATCTCTAATT TTAGAAGAAAAAAGTTTTTTCCCAAAAAGGAATTTAAATGTTATTTATACAACACTGATTTCACTTACCC AGACTGGTATAGAGCTCTGTAATGAAAATGATCCAGAACTTGCAAAAAGCTCTGATTCTCCTAGTTGAGA TAGTTTTTTGGTGATTTTTGGAATGTCTGTTTCAGCTGAATTCTCAAAATATTTTTCATTTTGAGTAGTT AAGGAATATGGTGCCTGAATTGAAGTTCCCATCTCCACTGTTCTTACACATAATCGAGAGTCAGTTTCCT CTACCTGTGGATTTATGTCTAAAACCCTACAGGTTTCATTTTTATTATCTTCAATGAGTCCTTGACTTTG TAACATTGTAACAACCCCTCTTTTTGATAGTTCTTCAAAGCCCATACTTTCCTTTCTGCTTTCTAGTTGG TGTTTTGTTGAGCACTCACTAGAGTTAGAACCACATGGAGTGCTATGCTGTACATTTAACTGATCAAATA CATTAGCTTCATCAGATGAACTGTAGACTGAACTTAAGTTTCCTATAGAAAGCTGGAAATGAACAATTTT TTTCTTGTTTTGTAATATTTCAGTATGTTCTTCAGAGGGATAGTTCTGATTTTTCATGCCTTGGGAAGAA TCTCTTGCTTCAATGCTAAAAATACTTGGTAGATCAGCATGCTGGCTCTCCTCCTGTGAAGAATATTCTG GCTGAAGCTGTTCAAAACATTGTTCAGATCCTGCATTAAGAAATTCAAAAATCAGTTAATCATCGTTACA TGTTGTAAAAGTTGGATACTGACAGTCTCTAATAGTTTATCATCCAATTGCAAGTAACCTACAATTTTAG GCATAAAAGTTGATGTGTATAAACATGAATACAAAGATAAGGATGGTCACAGTGATTTCTCAAACTACAG CTCACACCAAGTATTTTTTCTTTTTCTATTAATATTTAATAAGGACAATATGGCTATAAACTGAGGATGT TTTCCTCTATAAGAACTGAATATGACTTTAATAACAGAAAAGAGAATAAAGCATCCATGTGTATCTCAAT TAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL833615 unedited CAAATTTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGATTAAAGTAAGT TCTGGTTCTTCCATTATACCATGGCCAGTTTCTGTTTCCTGTGAACCACAATTAAAAGGA AAACCATGTCAAAATAGCCTTAAAAGACTTAAACTAATCGTTTTGTATATAATGCACATT TTTTGCAAGTGTATATAAATGTGAAAATGGAGGAAGAAAAGCAATGATTGGCAAAGACCA ACAGATCAGATTTCCTTAGGCTTCCATAACTCCAGCATTAGTTGTAGAAATACTGGAGCC TACTTTGGGGAGGGGATGAAATAGTGTGACAATGGACTGAAAGGTATCTACCACAATGAT TACAAACCTAAATGCTTGTAGTGTTGGGCAAAAAATATAAATGCAGGTTGGCTGTGCCGA ATTGTATCCTTTAAAGGGAGCAGGGGTCGACTGGGCTGCTACCAAATGTGGGCCACTGAC TGCTAACAGATAATGTTTCAAAAGTATCTAAACACAACAAGAACAAAAGCAGCCACATAA AATGTGGTTTAGTTGTAATTCTTATTTTCAATCTGTTATGTTTATTTGTGGAAGGATAAA CTGCACATACTGCTAGATGAAAACCAGTCACCAGTTGACTGGTGTTGAAAGCAAGCAGAT TATAAGTTGCTTAATATGAGGAGCAGGTTTCCAATACTTATTCCAATAATGGATGAATTC ATTGCACTGGAATGAGGCTCAACACTGAAAGTGCTGAAGCACAGGCAGAACCACCATTTT TCAGGGAAGTTGNAGAAAAGGATATCTGATTGGGAGAAAAAGGATCTTTTGTAAATACTC TCAAAGACAGTTNTCAAATTTTAAGTTCTGCATTATGCTGAATTNCTAGTGATCACAAGC TGGTAGCATATTACAAGCANTNTGCTGTATTTGNNTCAGTTTTTCTGCAGCATTGTGTGG TCAACTCTTCGAGTGAGCAGAGATC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL833615 unedited GTCCAACTATGNACCGCGGCACGCAATCTATGATCGAGTTTTTTTTTTTTTTTTTTATTG AGAACACATGGATGCTTTATTCTCTTTTCTGTTATTAAAGTCATATTCAGTTCTTATAGA GGAAAACATCCTCAGTTTATAGCCATATTGTCCTTATTAAATATTAATAGAAAAAGAAAA AATACTTGGTGTGAGCTGTAGTTTGAGAAATCACTGTGACCATCCTTATCTTTGTATTCA TGTTTATACACATCAACTTTTATGCCTAAAATTGTAGGTTACTTGCAATTGGATGATAAA CTATTAAAGACTGTCAGTATCCAACTTTTACAACATGTAACGATGATTAACTGATTTTTG AATTTCTTAATGCAGGATCTGAACAATGTTTTGAACAGCTTCAGCCAGAATATTCTTCAC AGGAGGAGAGCCAGCATGCTGATCTACCAAGTATTTTTAGCATTGAAGCAAGAGATTCTT CCCAAGGCATGAAAAATCAGAACTATCCCTCTGAAGAACATACTGAAATATTACAAAACA AGAAAAAAATTGTTCATTTCCAGCTTTCTATAGGAAACTTAAGTTCAGTCTACAGTTCAT CTGATGAAGCTAATGTATTTGATCAGTTAAATGTACAGCATAGCACTCCATGTGGTTCTA ACTCTAGTGAGTGCTCAACAAAACACCAACTAGAAAGCAGAAAGGAAAGTCTGGGCTTTG AAGAACTATCAAAAAGAGGGGTTGTTACAATGTTACAAAGTCAAGGACTCATTGGAGATA ATAAAAATGAAACCTGTAGGGTTTTTAGACATAAATCCACAGGTAGAGGAAACTGACTCT CGATTATGTGTAAGAACAGTGGAGATGGGGACTTCCATTCAGGCCCCTNATTCCTTAACT ACTCAAA
Restriction Sites	NotI-NotI
ACCN	AL833615
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL833615.1
RefSeq Size	3169 bp
RefSeq ORF	3169 bp
Locus ID	85459
Gene Summary	Centriole-enriched microtubule-binding protein involved in centriole biogenesis (PubMed:20844083, PubMed:25131205, PubMed:27185865). Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure (PubMed:27185865). Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles (PubMed:27185865). Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement (PubMed:25131205). Binds to and stabilizes centriolar microtubule (PubMed:27185865).[UniProtKB/Swiss-Prot Function]

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