RNF187 (BC008022) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RACO-1; RACO1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC008022, the custom clone sequence may differ by one or more nucleotides ATCGCAACCTGGGCCTCAGCATGCTGCTGCAGTGATGGCGCCAACCCGTGGCAGTCCCAGAGCTGGAGGC AGGAGGATGGATCCTCATCTCCATGGGAAGTGTCAGCGTGTGGCTGCCAGGGAAGCGTGGCAGGCGCCTG GCCTTGGGTCCATCTACATAGTTGCGTGTTTCAACAATGTCCATTTATCCTTCACCCCGAGGCGTGTTTT GGGGGCTGCAAACACCTCCCGGTAGAGGCTGGACCTGAGGACCCTTCCCACCTGTGCCCGTCCCTTCCTG AAGTCCTAGCCACAGCCCATCCTCCATGAGTCCCGGCAGCTCTGGGTCATGCCCTTCCCTGGTCACCCAT CTGCCCCTCACCTCGTCATCCAGGGACCCAGACCCTGCACCTTCCATGTGGGCCCACAGATCCTTGGCAG GTACCTGAGGTGCACCATTGAGTGTCGGATTTGGGGTTAGCATCCAGAAAGAAGAATGCGCATGACGCTC TGTGAAGGCTGGAACTCAGGTCTTCAGGGAGAGAAAGGAAGACTGGATTGCACCTTGATGCCTCCTGAGG AGGCGGCCCCCCTCTTGAGGTGGGCGTGGGCCCGGCCCAGCCTTATCCAAGTCGCTCTGTCCACCTCCCC CTTCCTGGCCCCCACCCCACTCCTGTGCCTCCCAGGAGCCCTCCCTGTGCTCCACCTGCCTCCGCAGAAG GAAGCCTCTTTCTCTGTTTCCCTGGGTGAGGGGGCTGGCAGGTGGCTAACCCCATTTAGCATCTCCAGGC CCTGCCATGGTGTCTCATCTTGCTGTTATCTCTAGCTCTTTCCCTCCTCCCATTTCCTTTAGTAGTTGAA TTTTGCAAAGCTTGTAGCAGTAGCTCAGTTGCCTGCAGCATCCTTGTGTGTAGATAAATTAGTCGACAGA AACTCAGCACTGGGGACAGGATTGCAAAGTCGGGGACATAGATGCAGACAGTTGTTGAGATTTGGGGATA GCCGGGCTTGTGAGCGGTGCCCATTTCCAGATGAAGCCTTTCAGCCCTTCTGAGTCCCCGGCCCTTGGTG CGATGTCTGTGAGTTTGACCTGCCCAGCGTGTGGGCTGGCTCAATGCTGAATAAAGTGGGTTTGTGTCAA AAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008022 unedited TTCAGCTTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGGTCTC GGAGCTGGAGAAGAAGCATCGCAACCTGGGCCTCAGCATGCTGCTGCAGTGATGGCGCCA ACCCGTGGCAGTCCCAGAGCTGGAGGCAGGAGGATGGATCCTCATCTCCATGGGAAGTGT CAGCGTGTGGCTGCCAGGGAAGCGTGGCAGGCGCCTGGCCTTGGGTCCATCTACATAGTT GCGTGTTTCAACAATGTCCATTTATCCTTCACCCCGAGGCGTGTTTTGGGGGCTGCAAAC ACCTCCCGGTAGAGGCTGGACCTGAGGACCCTTCCCACCTGTGCCCGTCCCTTCCTGAAG TCCTAGCCACAGCCCATCCTCCATGAGTCCCGGCAGCTCTGGGTCATGCCCTTCCCTGGT CACCCATCTGCCCCTCACCTCGTCATCCAGGGACCCAGACCCTGCACCTTCCATGTGGGC CCACAGATCCTTGGCAGGTACCTGAGGTGCACCATTGAGTGTCGGATTTGGGGTTAGCAT CCAGAAAGAAGAATGCGCATGACGCTCTGTGAAGGCTGGAACTCAGGTCTTCAGGGACAG AAAGGAAGACTGGATTGCACCTTGATGCCTCCTGGNGAGGCGGCCCCCCTCTTGAGGTGG GCGTGGGCCCGGCCCAGCCTTATCCAATCGCTCTGTCCACCTNCCCCTTNCTGGCCCCCA CCCCACTCCTGTGCCTNCCAGGAGCCCTCCCTGTGCTCCACCTGCCTCCGCAGAAGGAAG CCTCTNTCTCTGTTTCCCCTGGTGAGGGGGCTGGCANGTGGCTAACCCCATTTAGCATCT CCAGGCCCTGCCATCGNGTCTCATCTTGCTGGTATCTCTAGCTCCTTCCCTNCTCCCTTT NCTTTTATAGNTGAATTTTGCAAGCCTGTAGCAGTAGCTCAGTGCCTGCAGCATCCTGTG TGTGATAAATAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC008022 unedited AAATCACTGTGNACCGCGGCCCGCTATCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTGACACAAACCCACTTTATTCAGCATTGAGCCAGCCCACACGC TGGGCAGGTCAAACTCACAGACATCGCACCAAGGGCCGGGGACTCAAAAGGGCTGAAAGG CTTCATCTGGAAATGGGCACCGCTCACAAGCCCGGCTATCCCCAAATCTCAACAACTGTC TGCATCTATGTCCCCGACTTTGCAATCCTGTCCCCAGTGCTGAGTTTCTGTCGACTAATT TATCTACACACAAGGATGCTGCAGGCAACTGAGCTACTGCTACAAGCTTTGCAAAATTCA ACTACTAAAGGAAATGGGAGGAGGGAAAGAGCTAGAGATAACAGCCAGATGAGACACCAT GGCAGGGCCTGGAGATGCTAAATGGGGTTAGCCACCTGCCAGCCCCCTCACCCAGGGAAA CAGAAAAAGAGGCTTCCTTCTGCGGAGGCAGGTGGAGCACAAGGAGGGCTCCTGGGAGGC ACAAGAATGGGGTGGGGGCCAAGAAGGGGGAGGTGGACAGAGCGACTTGGATAAGGCTGG GCCGGGCCCACGCCCACCTCAAGAGGGGGGCCGCCTCCTCAGGAGGCATCAAGGTGCAAT CCAGTCTTCCTTTCTCTCCCTGAAGACCTGAGTTCCAGCCTTCACAGAGCGTCATGCGCA TTCTTCTTTCTGGATGCTAACCCCAAATCCGACACTCAATGGTGCACCTCAGGTACCTGC CAGGAATCTGTGGGCCCCACATGAANGTGCAAGGTCTGGNTCCCTGNATGACNAGGTGAG GGGCANATGGNTGACCAGGGAAGGGCATGACCCANACTGCCGGNACTCATGGAGGAGGNN CTGTGGCTAGACTTCAGAAGGNACGGCACCAGTGG
Restriction Sites	NotI-NotI
ACCN	BC008022
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008022.1
RefSeq Size	1144 bp
RefSeq ORF	1144 bp
Locus ID	149603
Gene Summary	E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.