PDZD11 (BC012996) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AIPP1; ATPase-interacting PDZ protein; OTTHUMP00000023469; OTTHUMP00000023470; PDZ domain containing 11; PDZK11; PISP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105917 sequence for BC012996 edited (data generated by NextGen Sequencing) ATGGACAGCCGGATTCCTTATGATGACTACCCGGTGGTTTTCTTGCCTGCCTATGAGAAT CCTCCAGCATGGATTCCTCCTCATGAGAGGGTACACCACCCGGACTACAACAATGAGTTG ACCCAGTTTCTGCCCCGAACCATCACACTGAAGAAGCCTCCTGGAGCTCAGTTGGGATTT AACATCCGAGGAGGAAAGGCCTCCCAGCTAGGCATCTTCATCTCCAAGGTGATTCCTGAC TCTGATGCACATAGAGCAGGACTGCAGGAAGGGGACCAAGTTCTAGCTGTGAATGATGTG GATTTCCAAGATATTGAGCACAGCAAGGCTGTTGAGATCCTGAAGACAGCTCGTGAAATC AGCATGCGTGTGCGCTTCTTTCCCTACAATTATCATCGCCAAAAAGAGAGGACTGTGCAC TAG Clone variation with respect to BC012996.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012996 unedited ACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGTCGCT GTGGACTGCCCACTGGGCCTTGCCCGAGATGGACAGCCGGATTCCTTATGATGACTACCC GGTGGTTTTCTTGCCTGCCTATGAGAATCCTCCAGCATGGATTCCTCCTCATGAGAGGGT ACACCACCCGGACTACAACAATGAGTTGACCCAGTTTCTGCCCCGAACCATCACACTGAA GAAGCCTCCTGGAGCTCAGTTGGGATTTAACATCCGAGGAGGAAAGGCCTCCCAGCTAGG CATCTTCATCTCCAAGGTGATTCCTGACTCTGATGCACATAGAGCAGGACTGCAGGAAGG GGACCAAGTTCTAGCTGTGAATGATGTGGATTTCCAAGATATTGAGCACAGCAAGGCTGT TGAGATCCTGAAGACAGCTCGTGAAATCAGCATGCGTGTGCGCTTCTTTCCCTACAATTA TCATCGCCAAAAAGAGAGGACTGTGCACTAGAAAGTTGCAGCCCACAGCCCTTCATGTGG ACTCTGTCATGACATGCTAACTAGACTTCAGGGGAGCCACTTCTGTTTTCAGCCCCTCCC TGNAATAGTGAGTTGGGAGGATGGGGAGACAGCTAACCAACTGCATTACCCANACCATAT TGCACTTTTAGTTCCCTAGTNTTCTANGTGAGCTTCATTCCCTGAAGGAGGATGATGATA TCTAGGCATAACCTAGCCTGTGAGGAACCTAGTTAGGAAAGACCACTGACATTTATTNGA TATCATGCACTAGTCCCTTACATATGTCATATTTTAATTATAGAAATCAGTAGCANAAGA ATCTGGNGGATTNTCATCTGACTTCCTGGCCATCTATNCCATCCTTGCACTACAGAGATN CTACACTTTGAGACTCAGTGAGAGCTGNNTTCACCCTTNCTCTNCTAGNCTTTNCCAAAA GTAANCCATGCTGAGAANAAAAAAAAAAAAAAANACTGACCTAATTGCGGCGGNCNAGNT TTNCTGACAAACCGGGGGATCCTGGACCTCCAAGCTTCTGGCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC012996 unedited GTACTCTATGNACCGCGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTCTTCAGCATTGTGTTTTACTTTTTGGGAGAAAGGCTAGGAGGAGGAAGGGGTGAA AACAGCGTCTCACTGGAGTCTCAAAAGTGTATGAATCTTCTGGTAGTGCAAGGATGGGAT AAGATGGCCAGGGAAGTCAGATGGAAAATCCCCAAGATTCTTTTTGCTACTGATTTCTAT AATTAAAATATGACATATGTAAGGGACTAGTGCATGATATTCAATAAATGTCAGTTGTCT TTCCTAACTAGGTTCCTCACAGGCTAGGTTATGCCTAGATATCATCATCCTCCTTTCAGG GAATGAAGCTCACCTAGAAAACTAGGGAACTAAAAGTGCAATATGGTTTGGGTAATGCAG TTGGTTAGCTGTCTCCCCATCCTCCCAACTCACTATTCCAGGGAGGGGCTGAAAACAGAA GTGGCTCCCCTGAAGTCTAGTTAGCATGTCATGACAGAGTCCACATGAAGGGCTGTGGGC TGCAACTTTCTAGTGCACAGTCCTCTCTTTTTGGCGATGATAATTGTAGGGAAAGAAGCG CACACGCATGCTGATTTCACGAGCTGTCTTCAGGATCTCAACAGCCTTGCTGTGCTCAAT ATCTTGGAATCCACATCATTCACAGCTAGAACTTGGTCCCCTTCCCTGCAGTCTGCTCTA TGTGCCTCAAAGTCAGGAATTCCCCTTGGAAATGAAGATGCCCTACCTGGAGGCCTTTTC TCCTCCGGAGTTAAAACCCCACTGAACTCCAGGAGGCTTCTCCTTGGGGATGTTCGGGGC CGAAACTGGGCCACTCTTTGTTGGTATCCCGGTGGGGGCACCTCTCTGAGGAGAATCCCT GCTGGAGGATTCTCATAGCCGGCAAA
Restriction Sites	NotI-NotI
ACCN	BC012996
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012996.2, AAH12996.1
RefSeq Size	967 bp
RefSeq ORF	423 bp
Locus ID	51248
Domains	PDZ
Protein Families	Secreted Protein
Gene Summary	Mediates docking of ADAM10 to zonula adherens by interacting with PLEKHA7 which is required for PLEKHA7 to interact with the ADAM10-binding protein TSPAN33.[UniProtKB/Swiss-Prot Function]

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