CMYA5 (BC020856) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2310076E16Rik; C5orf10; cardiomyopathy associated 5; DKFZp451G182; DKFZp451G223; genethonin-3; myospryn; SPRYD2; TRIM76; tripartite motif-containing 76
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC020856, the custom clone sequence may differ by one or more nucleotides AGTCGGAGGGAGAACACCAGGCGCGGCGCGGGCGGCTCCGGCTCCGGCCCCGGCCCAGGCCCGGGAGAGG CGATGGCGAGCCGCGATAGCAACCACGCTGGCGAGAGCTTTCTCGGCTCCGACGGGGACGAGGAGGCGAC CCGGGAGCTGGAGACCGAGGAGGAGTCGGAGGGCGAGGAGGACGAGACGGCGGCGGAGTCGGAGGAGGAG CCGGACTCCAGGTTATCAGACCAGGATGAAGAGGGAAAGATCAAGCAGGAGTATATCATATCTGACCCCT CCTTTTCCATGGTGACAGTCCAAAGGGAAGATAGTGGGATAACCTGGGAAACCAATTCAAGTAGATCTTC TACTCCTTGGGCTTCAGAAGAAAGTCAGACTTCTGGTGTGTGTAGTCGGGAAGGGTCAACTGTGAATTCT CCTCCTGGAAATGTTTCCTTTATTGTGGATGAAGTGAAAAAGGTTCGGAAAAGGACTCATAAGTCAAAGC ATGGTTCACCATCATTACGCCGGAAAGGCAACAGAAAAAGAAATTCTTTTGAATCCCAAGATGTTCCAAC AAACAAAAAAGGCAGTCCTTTAACTTCAGCAAGCCAGGTACTAACCACGGAGAAAGAGAAGTCATATACT GGCATTTATGATAAAGCAAGAAAAAAGAAGACCACTTCAAATACACCTCCGATTACTGGGGCAATATACA AAGAACACAAGCCATTAGTGTTAAGACCAGTCTACATAGGAACAGTACAATATAAAATTAAGATGTTTAA TTCGGTTAAAGAAGAATTAATTCCTCTACAATTTTATGGAACATTGCCAAAGGGTTATGTAATTAAAGAA ATACATTATAGGAAAGGGAAAGATGCATCCATTAGTCTAGAGCCAGATTTGGACAATAGTGGTTCTAATA CAGTGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020856 unedited TCAAAATTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGAGAACACC AGCGCGGCGCGGGCGGCTCCGGCTCCGGCCCCGGCCCAGGCCCGGGAGAGGCGATGGCGA GCCGCGATAGCAACCACGCTGGCGAGAGCTTTCTCGGCTCCGACGGGGACGAGGAGGCGA CCCGGGAGCTGGAGACCGAGGAGGAGTCGGAGGGCGAGGAGGACGAGACGGCGGCGGAGT CGGAGGAGGAGCCGGACTCCAGGTTCAATTAGCAGAATTTCTAGAAAATTTACAAGAAAA GTCCTTGAGGATTGAAGCCTTTGTTAGTGAGATAGAATCCTTTTTTAATACCATTGAGGA AAACTGTAGTAAAAATGAGAAAAGGCTAGAAGAACAGAATGAGGAAATGATGAAGAAGGT TTTAGCACAGTATGATGAGAAAGCCCAGAGCTTTGAGGAAGTGAAGAAGAAGAAGATGGA GTTCCTGCATGAGCAGATGGTCCACTTTCTGCAGAGCATGGACACTGCCAAAGACACCCT GGAGACCATCGTGAGAGAAGCAGAGGAGCTTGATGAGGCCGTCTTCCTGACTTCGTTTGA GGAAATCAATGAAAGGTTGCTTTCTGCAATGGAGAGCACTGCTTCTTTAGAGAAAATGCC TGCNTGCGTTTTCCCTTTTCGAACATTATGATGACAGCTCGGCAAGAAGTGANCAGATGT TAAAACAAGTGGGCTGTTCCACAGCCTCCTAGATTAGAACCTCAGGAACCAAATTCTGCC ACCAGCACAACAATNGCAGTTTACTGGAGCATGACANGGNAGATGTCNATGATCATNTCA GGTTACTGCTGGGAGAGCCACAGAGATCAGAGTAAATGAGTGGNGAAGATACGACTGACA TGAAGAAGCTCTGCATTTTGAGATCTGACTGACAGCTTTAAGGGGGGGGAGGGCTGGACT NACTGAT
Restriction Sites	NotI-NotI
ACCN	BC020856
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020856.1, AAH20856.1
RefSeq Size	948 bp
RefSeq ORF	948 bp
Locus ID	202333
Protein Families	Druggable Genome
Gene Summary	May serve as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) via binding to PRKAR2A (By similarity). May function as a repressor of calcineurin-mediated transcriptional activity. May attenuate calcineurin ability to induce slow-fiber gene program in muscle and may negatively modulate skeletal muscle regeneration (By similarity). Plays a role in the assembly of ryanodine receptor (RYR2) clusters in striated muscle (By similarity).[UniProtKB/Swiss-Prot Function]

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