MED29 (AL137304) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IXL; MED2
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106176 sequence for AL137304 edited (data generated by NextGen Sequencing) ATGGCTGCATCCCAGCAGCAAGCTTCAGCGGCTTCCTCAGCTGCTGGTGTATCGGGTCCT AGTTCGGCTGGCGGCCCGGGTCCCCAGCAGCAGCCGCAACCGCCAGCACAACTGGTGGGC CCTGCCCAGAGCGGCCTCCTGCAGCAACAGCAACAGGACTTCGATCCTGTGCAGCGTTAT AAGATGCTCATCCCGCAGCTGAAGGAGAGTCTACAGACCTTGATGAAGGTTGCGGCCCAA AACTTGATTCAGAACACTAACATCGACAATGGACAAAAGAGCAGTGATGGACCCATACAG CGCTTTGACAAGTGCCTGGAAGAGTTCTATGCACTCTGTGACCAGCTGGAGCTGTGCCTG CGCCTGGCGCATGAGTGCCTGTCACAGAGTTGTGACAGTGCCAAGCACTCTCCAACGTTG GTGCCCACAGCCACCAAGCCCGACGCAGTGCAGCCTGACAGCCTCCCCTACCCACAGTAC CTGGCGGTCATCAAAGCCCAGATTTCCTGTGCCAAGGACATTCACACCGCCCTGCTGGAC TGTGCCAACAAGGTCACGGGCAAGACACCCGCACCACCTGCTGGCCCTGGGGGCACTCTG TGA Clone variation with respect to AL137304.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL137304 unedited TTTTTACCCCCGCCCGACCNCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGGAAGATGGCTGCATCCCAGCAGCAAGCTTCAGCGGCTTCCTCA GCTGCTGGTGTATCGGGTCCTAGTTCGGCTGGCGGCCCGGGTCCCCAGCAGCAGCCGCAA CCGCCAGCACAACTGGTGGGCCCTGCCCAGAGCGGCCTCCTGCAGCAACAGCAACAGGAC TTCGATCCTGTGCAGCGTTATAAGATGCTCATCCCGCAGCTGAAGGAGAGTCTACAGACC TTGATGAAGGTTGCGGCCCAAAACTTGATTCAGAACACTAACATCGACAATGGACAAAAG AGCAGTGATGGACCCATACAGCGCTTTGACAAGTGCCTGGAAGAGTTCTATGCACTCTGT GACCAGCTGGAGCTGTGCCTGCGCCTGGCGCATGAGTGCCTGTCACAGAGTTGTGACAGT GCCAAGCACTCTCCAACGTTGGTGCCCACAGCCACCAAGCCCGACGCAGTGCAGCCTGAC AGCCTCCCCTACCCACAGTACCTGGCGGTCATCAAAGCCCAGATTTCCTGTGCCAAGGAC ATTCACACCGCCCTGCTGGACTGTGCCAACAAGGTCACGGGCCAGACACCCGCACCACCT GCTGGCCCTGGGGGCACTCTGTGAAGTGGNGGACAGGGAGTGGGGCANGCANTGGTTGGT GGGTGGTGTGCANAGGGAATGAANAGCGTTCTGGGCCCTAACACAGCAGCCTCCTCTCTT CCTGCCTGAGCACTGCAGCGGGAGCCCACCAGGGGGCACCAAAGGCAACAGGAGACTCGC AAGCCGGGCCCCTGCGTCCTGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL137304 unedited NCCCGTTAGCTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAG TTCTCAAACTTTTAATGTTTTTCAATAAAGTAACACGATTCTCCATATGCAGATGTTACA TTTCATGTGTTAGATTTGCTAAGTATGATTTAATTATACATTTCCATTTTTTGATCTTCA ACTTAATTACACTTAAAAAATGAGTATTTTGCTGGTGTAAACACCTCCACTTCTTTTTTT AAAAAAACAGGGTCTCCATGAACCAAGCTGGAGTGCAGTGGTGCGATCACAGCTCACTGC AGCTTCAACCTCTCAAGCTCAAGGCATCCTCCCACCTCTCAGCCTCCGAGTACCTGGGAA CCTGGGCACGCACCACCACACCTGGCTAATTTTTGAATTTTTTGCAGATAAGGGCTTTTG CTATGTTGCTCGGACTGGTCTTGAATTCCTGGGCTCAAGCCATCTGCCTACCTGCACCTC CCAAAGTGATGGGATTACAGGCCTGGGCCACTGTGCCTGGCCCCAACATCTCCACTTCTG ATCTCATTTTTCTGTTTTCTCACCATCTGATATATTTTACTTTTGTCACATTTACTGTCT CTCACTAGAACATCAGCTCCACAAATACAGGCCTTGGGCACAAGATACCCAATGTGTTTG CTGAGTGCAACTCAGTGGTGCCCACCTCCGNCCTGTGCCTGATAGATGTGTATCCATCAC CCCACCACCCACTGAGTGCCTCTGTGGGGCTCCCTGGGGTGTGGTTTTAAACATTGGTAG GCCCCTGTTTCATGGGTGCTGGGCTATTCAGCCCCCCCACCTGTGTGTGATGTTGTGTTG ACACCCCCCTGGGGAGGCCCGACAGTCCCTCCAGTTTGTGGC
Restriction Sites	NotI-NotI
ACCN	AL137304
Insert Size	3700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL137304.1, CAB70687.2
RefSeq Size	3539 bp
RefSeq ORF	603 bp
Locus ID	55588
Gene Summary	MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]

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