VPS50 (AL833112) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC132; VPS54L
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106299 sequence for AL833112 edited (data generated by NextGen Sequencing) ATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCT GCCAGCACTTTTAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACT TTGGAACAGATTGAGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGAC ATTAACCATTATACCAAGGTTCAACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCA ATGGATCAACTTCATATGCACTTCACCCAAGCCATTCACAACACCGTGTTTCAAGTTGTT CTTGGTTATGTGGAACTATGTGCAGGAAACACAGACACAAAATTCCAAAAGCTGCAATAT AAGGATCTCTGTACACATGTTACACCAGACAGCTATATTCCATGCCTTGCAGACCTGTGC AAAGCACTATGGGAAGTTATGCTCAGCTATTATAGGACTATGGAATGGCATGAAAAGCAT GACAATGAGGATACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAAACT AATTTTGATCGTGGCTACATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAG GATGTTCAGCTAAAAGTAAAAACCTACTTGCTTGGAACTGATTTGTCTATATTCAAATAT GATGATTTCATCTTTGTTTTGGATATAATCAGCAGGTTGATGCAAGTTGGAGAAGAATTT TGTGGTAGCAAGTCTGAAGTTTTACAGGAATCTATTAGAAAACAAAGTGTCAATTATTTC AAGAATTACCATAGAACACGGCTCGATGAACTGAGAATGTTCTTAGAGAATGAGACTTGG GAACTTTGTCCTGTTAAGTCAAATTTCAGCATCTTGCAACTTCATGAATTTAAATTCATG GAACAGTCTCGCTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACTTCTTCAAAA ACAGTGACCTTGTTTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAAC CACAAAGATGAAGAAACAGAAGATGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAA GAAAAGAGTGCCTATCAAGAGTATGACAGTGACAGTGATGTTCCTGAGGAACTCAAACGA GACTATGTGGATGAGCAGACAGGAGATGGTCCTGTGAAAAGTGTTTCTCGGGAAACTCTA AAAAGCAGGAAGAAATCAGATTACAGTCTAAATAAAGTGAATGCACCTATCTTAACAAAT ACAACATTGAACGTCATAAGACTTGTTGGAAAATATATGCAGATGATGAACATTCTTAAG CCAATTGCCTTTGATGTTATTCATTTCATGTCTCAACTATTTGATTATTACTTGTATGCA ATATATACCTTTTTTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTTAGTAGT AGTAGACTAAGAACAACTCTAAACAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCA GCTGATCCTACTGCCACACTCACAGCAGCAGAAGAAAGAAAGGAGAAGGTGCCAAGTCCA CACCTCAGTCACCTAGTGGTTTTGACATCTGGGGATACGCTGTATGGGTTGGCAGAAAGA GTGGTAGCCACGGAATCCTTGGTATTCTTGGCTGAACAGTTTGAGTTCCTTCAGCCACAT CTGGATGCTGTGATGCCTGCAGTCAAAAAGCCCTTTCTTCAGCAGTTCTATTCTCAGTCT CAACCGCCAGTGAACTACGGAAACCAATTTACTGGATTGTAG Clone variation with respect to AL833112.1 1600 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL833112 unedited TGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAAAAGTTACCTCA TTGCAGACAGGTCTTCAATTAGCTGCTGTTTTCTGTACAAATGGGAGAAGACACTTGAAT ATTGCAAAGGAAGGTTTTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGT CAGTTGCTGATTGGACTTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGAT GTACGGTTAAGTGAAATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTT GAATGTCAAAAAGCTGCCAGCACTTTTAAACATTACAGTTGTATAAGTGAACTGAATTCA AAGCTGCAAGATACTTTGGAACAGATTGAGGAACAGCTGGACGTAGCTCTTTCCAAAATC TGCAAGAATTTTGACATTAACCATTATACCAAGGTTCAACAAGCTTATCGACTTCTTGGA AAAACACAGACAGCAATGGATCAACTTCATATGCACTTCACCCAAGCCATTCACAACACC GTGTTTCAAGTTGTTCTTGGTTATGTGGAACTATGTGCAGGAAACACAGACACAAAATTC CAAAAGCTGCAATATAAGGATCTCTGTACACATGTTACACCAGACAGCTATATTCCATGC CTTGCAGACCTGTGCANAGCACTATGGGGAGTTATGCTCAGCTATTATAGGACTATGGAA TGGCATGAAAAGCATGANCATGAGGATACTGCTTCANCTTCTGAANGGGAGTATATGATA NGTACTGAAGAAACTAATTTTGATCGTGGCTACATAAAAAAGAAATAGAACATGGACTTA CACGAATATGGCAGGGATGTCAGCTAAAGTAAAAACTACTTGCTNGAAAACTGATTGCTA TATTCAATATGN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL833112 unedited GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGGCTGATCATACTA GAGTATATTTATTAAATTTCACCATTATGATCACTTTGTTATAACTAAATTAACCCTGAC ATAAACTTTGACATTTTAAAAACAGAGAACATACTGATTTTTCAACTATTTTACTTACAT GTGTTTTTTAATTCTCATGCAGTGCTCATAATTAGGAGATACTACATATTCACCCTCACA TTATGCTTATTAAGAACCAAGCTCAGAGGGTAGAAAAGTGCCAACTTCATTTTCCATAAA CTTCCCATACATATATTAAGCCACTGTTCTTCTAATTATATACTTATGGTACATTCTTCT GTAAAATGTATAACTGTAGTCACATAACCATTGTTATGACAAGGCGGCAACATCATTTCA GATTACAACAAATGAGACCAAAAGTTGAAGAGACCACAGCATATAAAAGTCATGAGAACA CCACACACATTTCAATATTTTCCACTTTCCAGTCAAAAAAACATGCACAAAGTTTTCTTA GCAGATAGTTGTCAGAAGTGCATAAAAAAACTGGCTTTCAGGTCATATATGTTGAGTGAG GATCAATGCCATTGAGGAAAGAGAGCTGTGTTCATTATCTTTTANGTCTGTCTATATCAT CTATAGCTGCTAGAAGTTTTTGTCTTGCTTTCTTATTGATATGGGATCCCAGGCAAACAT TCACCAGATTGGTCAGCTGCTTCGTTGAATATTCCCTGTGCTCTTTGATCCACCGTTCCA TGTCATTCTCAGTTAGGTAATAAAGCTTAATATAAGTTTCTACAAATTCTTATCANGAAT GGGGTCTATATCTCGTAAGTTTTTCAGTTTCATAAAAACTGTTGAAAATCCATTGATCAG GNCACGACCCTCATACTGCATTCTNGACTTGNCTATCCTTTACATN
Restriction Sites	NotI-NotI
ACCN	AL833112
Insert Size	2900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL833112.1, CAI46135.1
RefSeq Size	3505 bp
RefSeq ORF	1782 bp
Locus ID	55610
Gene Summary	Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function]

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