FBXO11 (AF174599) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FBX11; PRMT9; UBR6; UG063H01; VIT1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF174599, the custom clone sequence may differ by one or more nucleotides CGGCCGCGCCCGGTGCAGCAACAGCAGCAGCAGCCCCCGCAGCAGCCGCCGCCGCAGCCGCCCCAGCAGC AGCCGCCCCAGCAGCAGCCTCCGCCGCCGCCGCAGCAGCAGCAGCAGCAGCAGCCTCCGCCGCCGCCACC GCCGCCTCCGCCGCTGCCTCAGGAGCGGAACAACGTCGGCGAGCGGGATGATGATGTGCCTGCAGATATG GTTGCAGAAGAATCAGGTCCTGGTGCACAAAATAGTCCATACCAACTTCGTAGAAAAACTCTTTTGCCGA AAAGAACAGCGTGTCCCACAAAGAACAGTATGGAGGGCGCCTCAACTTCAACTACAGAAAACTTTGGTCA TCGTGCAAAACGTGCAAGAGTGTCTGGAAAATCACAAGATCTATCAGCAGCACCTGCTGAACAGTATCTT CAGGAGAAACTGCCAGATGAAGTGGTTCTAAAAATCTTCTCTTACTTGCTGGAACAGGATCTTTGTAGAG CAGCTTGTGTATGTAAACGCTTCAGTGAACTTGCTAATGATCCCAATTTGTGGAAACGATTATATATGGA AGTATTTGAATATACTCGCCCTATGATGCAT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF174599 unedited TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCTGCCCCGAA GCTCGCTGTGCGTGTGCGTGTGTGTGTCCCTCCGCCAACGCCGCCACCTCAGCCCGGCAA ATGAACTCCGTCCGAGCCGCCAACCGGAGACCCAGGCGAGTGTCGCGGCCGCGCCCGGTG CAGCAACAGCAGCAGCAGCCCCCGCAGCAGCCGCCGCCGCAGCCGCCCCAGCAGCAGCCG CCCCAGCAGCAGCCTCCGCCGCCGCCGCAGCAGCAGCAGCAGCAGCAGCCTCCGCCGCCG CCACCGCCGCCTCCGCCGCTGCCTCAGGAGCGGAACAACGTCGGCGAGCGGGATGATGAT GTGCCTGCAGATATGGTTGCAGAAGAATCAGGTCCTGGTGCACAAAATAGTCCATACCAA CTTCGTAGAAAAACTCTTTTGCCGAAAAGAACAGCGTGTCCCACAAAGAACAGTATGGAG GGCGCCTCAACTTCAACTACAGAAAACTTTGGTCATCGTGCAAAACGTGCAAGAGTGTCT GGAAAATCACAAGATCTATCAGCAGCACCTGCTGAACAGTATCTTCAGGAGAAACTGCCA GATGAAGTGGTTCTAAAAATCTTCTCTTACTTGCTGGAACAGGATCTTTGTAGAGCAGCT TGTGTATGTAAACGCTTCAGTGAACTTGCTAATGATCCAATTTTGTGGAAACGATTATAT ATGGAAGTATTTGAATATACTCGCCCTATGATGCATCCTGAACCTGGAAAATTCTACCAG ATTAATCCAGAAGAGTATGAACATCCAAATCCCTGGAAAAGAGAGTTCCAGCANGTGTAT AANAGTGCACATNGTAAGCCAGGATTTGCTGAACATTTCTACAGTACN
Restriction Sites	NotI-NotI
ACCN	AF174599
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF174599.1, AAF04520.1
RefSeq Size	591 bp
RefSeq ORF	591 bp
Locus ID	80204
Domains	F-box
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]

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