RNF123 (AL832034) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FP1477; KPC1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832034, the custom clone sequence may differ by one or more nucleotides CCTGAACGGACGCCGTAGCCAAGAGGTTGGGCGGATGTTGTGAGCCGGGTCGCTGCGGCCGAGGCTCCGG CCCCCAGGACCACTGGCTGCCCATGAGAGATGAAGGATGGCATCCAAGGGGGCCGGCATGTCTTTCTCCC GCAAGAGCTATAGGCTGACCTCAGATGCTGAGAAATCCAGGGTCACAGGCATTGTGCAGGAGAAGCTGCT GAATGACTACCTGAACCGCATCTTTTCCTCTTCTGAACATGCACCCCCAGCAGCCACCAGCAGGAAACCC CTGAACTTCCAGAACCTGCCAGAACATTTGGACCAGTTGCTACAGGTGGACAATGAGGAGGAGGAAAGCC AGGGACAGGTTGAAGGGCGGCTTGGCCCATCCACTGTGGTCCTGGACCACACAGGCGGCTTTGAGGGGCT TCTCCTGGTGGATGATGACCTGCTGGGGGTGATTGGACACAGCAACTTTGGCACCATCCGCTCTACCACA TGCGTGTACAAAGGGAAATGGCTCTACGAGGTCCTCATCTCCTCCCAGGGGCTCATGCAGATCGGCTGGT GCACCATCAGCTGCCGCTTCAACCAGGAGGAGGGGGTTGGAGATACACACAACTCCTATGCCTATGATGG CAACCGCGTGCGCAAGTGGAATGTGACCACAACGAATTATGGCAAGGCGTGGGCAGCGGGGGACATCGTG AGCTGCCTGATTGACCTGGATGATGGCACTCTGTCCTTCTGCCTGAACGGTGTATCACTGGGCACTGCCT TTGAGAACCTGTCCAGGGGCCTGGGTATGGCCTACTTCCCAGCCATCAGCCTCTCTTTCAAGGAGTCCGT GGCCTTCAACTTTGGCAGCCGTCCTCTGCGCTACCCAGTGGCAGGCTACCGGCCCCTGCAGGACCCACCG AGTGCTGACCTGGTGCGGGCACAGAGGTTGCTGGGCTGCTTCCGGGCAGTGCTGAGTGTGGAGCTGGACC CTGTGGAGGGGCGGCTGTTGGACAAGGAGAGCTCCAAGTGGCGGTTGCGGGGCCAGCCCACCGTCCTCCT CACACTGGCCCACATCTTCCATCACTTTGCACCGCTTCTGCGCAAGGTGTATCTGGTGGAGGCTGTGCTC ATGAGCTTCTTGCTGGGCATCGTGGAGAAGGGCACACCCACACAGGCACAGTCCGTGGTGCACCAGGTCC TGGACCTCTTGTGGCTCTTCATGGAGGACTACGAGGTACAAGATTGCCTCAAGCAGTTGATGATGTCTCT GCTTCGGCTGTACCGATTCTCACCCATTGTCCCAGACCTGGGCCTACAGATCCATTACCTGCGGCTCACT ATCGCCATCCTGAGGCATGAGAAGTCCCGCAAGTTTCTGCTTAGCAATGTCCTCTTCGACGTGCTCCGCT CCGTCGTCTTCTTTTACATCAAGAGCCCCCTGCGTGTGGAGGAGGCCGGCCTGCAGGAGCTCATTCCCAC CACCTGGTGGCCCCACTGCTCCAGTAGGGAGGGCAAAGAGAGCACGGAGATGAAGGAGGAGACCGCAGAG GAGCGGCTGCGGCGGCGAGCCTACGAACGGGGCTGTCAGCGGCTCAGGAAGCGCATCGAAGTGGTGGAAG AACTACAGGTCCAGATCCTGAAGCTGCTGCTGGACAATAAAGATGACAATGGGGGTGAAGCTTCTAGGTA TATCTTCCTGACCAAGTTTCGCAAGTTTCTGCAGGAGAACGCCAGTGGCCGGGGGAACATGCCCATGCTC TGCCCCCCTGAGTACATGGTCTGCTTCTTACACCGGCTGATCTCTGCCCTGCGCTACTATTGGGATGAAT ACAAGGCTTCCAATCCTCATGCTTCCTTCAGTGAGGAGGCCTACATCCCGCCCCAGGTCTTCTATAATGG CAAGGTGGACTACTTTGACCTGCAGCGCCTGGGGGGCCTCCTCTCGCGCCTGCGGAAGACCCTCAAAGAT GACCTTGCTTCCAAAGCCAACATTGTGATCGACCCACTGGAGCTCCAGTCAACCGCCATGGATGACCTAG ATGAGGATGAGGAGCCAGCCCCAGCTATGGCCCAGCGCCCCATGCAGGCCCTGGCTGTTGGGGGGCCACT GCCCCTGCCCCGGCCCGGCTGGCTCAGTTCTCCAACTTTGGGCCGAGCCAACCGCTTCCTCAGCACAGCG GCTGTGAGCCTCATGACCCCACGGCGGCCTCTGAGCACCTCGGAGAAAGTGAAGGTCCGCACGCTGAGCG TGGAGCAGAGGACCCGTGAGGACATTGAAGGCAGCCACTGGAATGAGGGCTTGCTGCTGGGGCGGCCCCC CGAGGAGCCTGAGCAGCCCCTCACCGAGAACTCGCTGCTGGAAGTCCTGGATGGGGCGGTCATGATGTAC AACCTCAGCGTACACCAGCAGCTGGGCAAGATGGTGGGTGTCTCCGATGATGTCAATGAATACGCTATGG CTCTGAGGGGCACAGAGGACAAGCTCCGCCGGTGCCCCAAGAGGAGGAAGGACATCCTTGCAGAGTTGAC CAAGAGCCAGAAGGTTTTCTCAGAAAAGCTGGACCACCTGAGCCGCCGTCTTGCCTGGGTCCATGCCACT GTCTACTCCCAGGAGAAGATGCTGGACATCTACTGGCTGCTGCGCGTCTGCCTGCGGACCATTGAGCACG GTGATCGCACAGGGTCTCTCTTTGCCTTCATGCCCGAGTTCTACCTGAGCGTGGCCATCAACAGCTACAG TGCTCTCAAGAATTACTTTGGTCCCGTGCACAGCATGGAGGAGCTCCCAGGCTATGAAGAGACCCTGACC CGCCTGGCTGCCATTCTCGCCAAACACTTTGCCGACGCACGCATTGTGGGCACTGACATCCGAGACTCAC TGATGCAGGCCCTGGCCAGCTACGTGTGCTACCCACACTCCCTGCGGGCTGTGGAGCGAATCCCCGAGGA GCAGCGTATCGCCATGGTGAGGAACCTCCTGGCGCCCTATGAGCAGCGGCCCTGGGCCCAGACCAACTGG ATCCTGGTGCGGCTCTGTAGGGGCTGTGGCTTCGGGTACCGCTATACACGGCTGCCACATCTGCTGAAAA CCAAACTTGAGGACGCCAATTTGCCCAGCCTCCAGAAGCCCTGCCCTTCCACCCTGCTGCAGCAGCACAT GGCGGACCTCCTACAGCAGGGTCCTGATGTGGCACCCAGCTTCCTCAACAGCGTCCTCAATCAGCTCAAC TGGGCCTTCTCTGAATTCATTGGCATGATCCAAGAGATCCAGCAGGCTGCTGAGCGCCTGGAGCGGAACT TTGTGGACAGCCGGCAGCTCAAGGTATGTGCCACCTGCTTTGACCTCTCGGTCAGCCTGCTGCGTGTCTT GGAGATGACTATCACACTGGTGCCTGAGATATTCCTTGACTGGACCCGGCCTACCTCTGAGATGCTGCTG CGGCGTCTTGCACAGCTGCTAAACCAGGTGCTGAACCGGGTGACAGCTGAGAGGAACCTGTTTGATCGTG TGGTCACCCTACGGCTGCCTGGCCTAGAGAGCGTGGACCACTATCCCATTCTGGTGGCAGTGACGGGCAT CCTGGTGCAGCTCCTGGTGCGTGGCCCAGCCTCAGAGAGAGAGCAAGCCACATCAGTGCTCCTGGCAGAT CCCTGCTTCCAGCTACGCTCAATATGCTATCTCCTGGGACAGCCAGAGCCCCCAGCACCTGGCACTGCTC TGCCAGCCCCTGACCGGAAGCGCTTCTCCCTGCAGAGCTATGCGGATTATATCAGTGCCGATGAGCTGGC CCAAGTGGAACAGATGCTGGCGCACCTGACCTCTGCATCTGCCCAGGCAGCAGCTGCCTCCCTGCCCACC AGTGAGGAGGACCTCTGCCCCATCTGCTATGCCCACCCCATCTCTGCTGTGTTCCAGCCCTGTGGCCACA AGTCCTGCAAAGCCTGTATCAACCAGCACCTGATGAACAACAAGGACTGCTTCTTCTGCAAAACCACCAT CGTGTCTGTAGAGGACTGGGAGAAGGGAGCCAATACGAGTACTACCTCCTCAGCTGCCTAGCCCTCACAG CCTGTGCCATCCTGGAACCTCCACCTTTGAACCCAGAGCCAGGCTGGGCCCTATTTATGGGCTCCCTTTG CCCTTCTCCTGTATCCCACACCACCACATCCAACCTCCTTGCCTGCCTGTATCCTCATTGGTGGGAGCCC AGCCATGGCCCTAATTGTGCCTGAGCTTGACTTTCAGTCAGGGCCACAGTGAGCATTAAATTATTATTCC ATAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832034 unedited CTCACTATAGGGGCGGCCGCGATTCGGCACGAGGGGAGAGAGAGAGCCGGGTCGCTGCGG CCGAGGCTCCGGCCCCCAGGACCACTGGCTGCCCATGAGAGATGAAGGATGGCATCCAAG GGGGCCGGCATGTCTTTCTCCCGCAAGAGCTATAGGCTGACCTCAGATGCTGAGAAATCC AGGGTCACAGGCATTGTGCAGGAGAAGCTGCTGAATGACTACCTGAACCGCATCTTTTCC TCTTCTGAACATGCACCCCCAGCAGCCACCAGCAGGAAACCCCTGAACTTCCAGAACCTG CCAGAACATTTGGACCAGTTGCTACAGGTGGACAATGAGGAGGAGGAAAGCCAGGGACAG GTTGAAGGGCGGCTTGGCCCATCCACTGTGGTCCTGGACCACACAGGCGGCTTTGAGGGG CTTCTCCTGGTGGATGATGACCTGCTGGGGGTGATTGGACACAGCAACTTTGGCACCATC CGCTCTACCACATGCGTGTACAAAGGGAAATGGCTCTACGAGGTCCTCATCTCCTCCCAG GGGCTCATGCAGATCGGCTGGTGCACCATCAGCTGCCGCTTCAACCAGGAGGAGGGGGTT GGAGATACACACAACTCCTATGCCTATGATGGCAACCGCGTGCGCAAGTGGAATGTGACC ACAACGAATTATGGCAAGGCGTGGGCAGCGGGGGACATCGTGAGCTGCCTGATTGACCTG NATGATGGCACTCTGTCCTTCTGCCTGAACGGTGTATCACTGGGCACTGCCTTTGAGACC TGTCCAGGGCCTGGGTATGGCCTACTTNCCACCATCAGCCTCTCTTTCAGGAGTCCGTGC CTTCACTTGGCAGCCGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832034 unedited CCCCCCAATGNGGATAATTAACCGCATCGGTGGTTCCTGAAAGAACATCAAGCTCAGGCA CAATTAGGGCCATGGCTGGGCTCCCACCAATGAGGATACAGGCAGGCAAGGAGGTTGGAT GTGGTGGTGTGGGATACAGGAGAAGGGCAAAGGGAGCTCATAAATAGGGCCCAGCCTGGC TCTGGGTTCAAAGGTGGAGGCTCCAGGATGGCATTGGTTGTGAGGGCTAGGCAGCTGAGG AGGTAGTACTCGTATTGGCTCCCTTCTCCCAGTCCTCTACAGACACGATGGTGGTTTTGC AGAAGAAGCAGTCCTTGTTGTTCATCAGGTGCTGGTTGATACAGGCTTTGCAGGACTTGT GGCCACAGGGCTGGAACACAGCAGAGATGGGGTGGGCATAGCAGATGGGGCAGAGGTCCT CCTCACTGGTGGGCAGGGAGGCAGCTGCTGCCTGGGCAGATGCAGAGGTCAGGTGCGCCA GCATCTGTTCCACTTGGGCCAGCTCATCGGCACTGATATAATCCGCATAGCTCTGCAGGG AGAAGCGCTTCCGGTCACGGGCTGGCAGAGCAGTGCCAGGTGCTGGGGGCTCTGGCTGTC CCAGGAGATAGCATATTGAGCGTAGCTGGAAGCAGGGATCTGCCAGGAGCACTGATGTGG CTTGCTCTCTCTCTGAGGCTGGGCCACGCACCCAGAGCTGCACCAGGATGCCCGTCACTG CCCCCAAAAGGGGTAGTGGTCCCAGCTTCTTTAGCCCAGGCAGCCGTAGGGTGCCACCCG ATCAAACAGGGTCCTCTCAGCTGTCACCCGGTTCAACACCTGGTTTTAACACCTGTTCCA AGGACGCCGCACGAGCA
Restriction Sites	NotI-NotI
ACCN	AL832034
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832034.1
RefSeq Size	4284 bp
RefSeq ORF	4284 bp
Locus ID	63891
Domains	RING, SPRY
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

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