Calpastatin (CAST) (AL832349) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BS-17; PLACK
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832349, the custom clone sequence may differ by one or more nucleotides CACTCAGAGAGCTGGGCTGGAGCTCCAAGCGGAAACCGCGGGAGCCGAGCCCAGCTAGGAATGCAGACCT CCTGAAAACCAAGCCGAGGACTGCGGGGTCCGGTGTCCACGCAGAGTGTCAGCTTCCTCTGGTGCAACCA GCAAGTCTTCCAGTATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTC AACCAAGCTGTCTGTGGTTCATGAGAAAAAATCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAA AGCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAG CTGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGG AGAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTA ACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGG ATGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCAGA AGTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAA TATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGC CAGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAAC TGAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCC CAAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCAC TGGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAA AGAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCC ACGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAAC TCATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAA GACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTCTCGGACCTCCATGTGTAGT ATACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGG CTGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAA GGCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAA GAGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAATAGCTTCCACCCATGAGTG AAGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGA AGATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGA GCCCCACCCCGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTC TAGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGC TGAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGAT AATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACC AAGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACAC AGCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGAT TCAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGACTAAAGAAATACAAGTTAAGGTATCTGGT ATCTGCATGTAAAATCTTCAGCTGGTGGATGGTGACTTTTGAAGAACAAAAGGCTTTGGCAACAGAAAAC AATTGTTCTGGGTGATTTCTAGAATGGTTTTTGTTGAGTCTCTGAACATCCTAAATATTGGTTTGTTATT CTTTTCCAGAAAGAAAATGAATTTGACTGGTTCACCTGTGTACTGAGTATTGATAAACTTTGAATTTTTT TAATTGCCTTCAATTGGGAGAGAAAGCTTTATATTTGTAAGAAATATATTTGATAAAGTTTCTTAAAGCA ACACCAAAAAAACAAAAGAAAAGCTAAGTGAATTTTTGCACATTCTACACACAGTGCCTGTAAATCTCAT TTGTATTTTCAGTTTGCCCTTAATTTTTTTTGTTAGTGTTTAGAAAACAATGTTTTAAACATTCTTCAGT GTTCTGATTTCTTATTACCCCCTTTCCTCTTGGGCTTTTGAACTGTATTTGATGTTGCTTTGGGATAATG TTTATAAGTCAAACATAAGATATTGTACATTGGGCACATATCTCCTCTTGGGCTGCTAATAATAAATTAA TAACAGGTAACCTGGACAAACCAGGAAGCACCAAACCCCTTTTCAGTTTGAACTCTTCTTTGCCAGGTGT GAGGACTTCTGCATCTTACAGTCAGCACAGAACACACTGAGACTTGAATCAAGTCAGCAACAGAGCAAAA TAAAGGTTAGATAAGTCCTTGTGTAGCAAATTTCGAGCATAAGAAATAAAATCTAATTAATTCTTAGGGT ACTCATCTGACTTGAACTCTGTTGGTTTACTGTGTTAGTAAACTGTGCTTTCTATTATCTATACATAAAA CCTGAGCAGCAACTGTGTCTTTAGAGCTATTGCCACATTAGCCTTTGCACTGTATAGCATCTGGCTTTAT GGAACTTAAGTTTACCAAATATAAAAAGAAACTTCTGCTTTTAAAAAAATTATATATATATATTAAATTT GAAACCTGCATTTCTCCCACAGCAATGTAAGAAGTAGGCTCTGATGTCCTACCACTTTGAATGGTTTTCT AATATCTTAATGAATAGTTCCTGAACATTGCACTGATATCATCGATTAGAATTTTGATATTTAATTTCAT CTTTATTTCCTGGTAGAGAATGCAGGAAAAGATGTCAGGTACATAACATAAAACAGATTGGGAATTTATT GTTTCCAAAGGGCATGGCCTTCCTTAGCATCAGTTTGAAGCTTTTGTTATGACTTAGCTGACTTGTGGCA GCGGAGCAAGCAAAAACAATAACACTGCTTATAAATGGCACCACATCTTGTTAACCTCCCCCCCAAATAC TCTCTGAAAGTCATGCACATACCTATGGGATTTTACACACCACCAGCTTAAAATGCTATGTCTCTATCCA TCAGAAATAGTCATTATTCTATTTTTAAGGCAGCAACAAGAAAAGAAAAAACACTTTTCCTGAGGGATTT CTAACCATGTATCTAATCCTCCCATTTGGGCAGTATAGGTGTTTGCTTTTTTGTTTTCTTTTTTTAAGAA AAACCTTGAAACCTTTGACACTGACAGATGTGTTTGCAAGGATACGGCTGCAGTATTACTAATTTCCATG TGTATCTGGAAGTATTTTTAAATGGCATACCAAAATCCAGAAGTTTAAAGATGCCTATAAAAGTAAACAA CATTTATTTAAAAAGAACTCTGAATATGCCTTCTTTTTTAATTAGAAATATCTTCGAGACTTGGGTGTTT GTTAATAACTAATAACTGGAGTAAGCTACAGGATCTAAAGCAGCCCTTTTTACAGTCTAGTTAGGAGAGA GAAAATAATTGCAAATATCCACTTAGAGGCAAAGAACAATTTTTTTTTATCAAAAAGGTTTCTGCACATT GTTGTGGCAATATTGTATCTGTTTAGAAAATGGGCTTTTCCAAAAGCAAACAAAGATAGGTTCCTCAGGT GACCAAAACTGAAAATCAATATTTCCATGTTTCATTAATCAAGGCATAAAATACAATTAAAGCAAAATAT TTTACATTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832349 unedited GATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGCCGCCCCGTCGC ACTCAGAGAGCTGGGCTGGAGCTCCAAGCGGAAACCGCGGGAGCCGAGCCCAGCTAGGAA TGCAGACCTCCTGAAAACCAAGCCGAGGACTGCGGGGTCCGGTGTCCACGCAGAGTGTCA GCTTCCTCTGGTGCAACCAGCAAGTCTTCCTTTATGAATCCCACAGAAACCAAGGCTGTA AAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCTGTCTGTGGTTCATGAGAAAAAA TCCCAAGAAGGAAAGCCAAAAGAACACACAGAGCCAAAAAGCCTACCCAAGCAGGCATCA GATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAG CCATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGA GAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAG AAATCATTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCA GGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTANGAGGACCTGAAGAAAACTGA GAAGAAAATANCACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTTCACCTACATA GAGGAATTGGGTAAAAGAGAAGTCACATTTCTCCNAAATATAGGGAACTATTGCCTAANA AGGAAAGGATCACAGGNCCTCCTGCAGACTCTTCGAAACCCATAGGCCAGATGATGCTTT ANACGCCTGTCATCTGACTTCAACTGTGGGTCGNCTACAGCTGCTGGGAAGAAAAACTGA AAAGAGGATCTACAGAATTTTAAAAGCTTATTCCNCAGGGCAGTCAGAGTGCTGCTCCAC CCNAGAAAGAAAGAAAGTGGAAAAGAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832349 unedited GTACATTATGNACGGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTGGGGCTTCCTGGTTTGGCCAGGTTACCTGGTATTAATTTATTATTAGCAGCCC AAGAGGAGATATGTGCCCAATGTACAATATCTTATGTTTGACTTATAAACATTATCCCAA AGCAACATCAAATACAGTTCAAAAGCCCAAGAGGAAAGGGGGTAATAAGAAATCAGAACA CTGAAGAATGGTTAAAACATTGGTTTCTAAACACTAACAAAAAAAATTAAGGGCAAACTG AAAATACAAATGAGATTTACAGGCACTGTGTGTAGAATGTGCAAAAATTCACTTAGCTTT TCTTTTGTTTTTTTGGAGTTGCTTTAAGAAACTTTATCAAATATATTTCTTACAAATATA AAGCTTTCTCTCCCAATTGAAGGCGATTAAAAAAATTCAAAGTTTATCAATACTCAGTAC ACAGGTGAACCAGTCAAATTCATTTTCTTTCTGAAAAAGATAACAAACCAATNTTTAGGA TGGTCAGAGACTCAACAAAAACCATTCTAGAAATCACCCAGAACAATTGTTTTCTGTTGC CAAAGCCTTTTGTTCTTCAAAAGTCACCATCCACCAGCTGAAGATTTTACATGCAGATAC CAGATACCTTAACCTGTATTTCTTTAGTCATCCTTTTGCTTGGAAGTTTCTCCGGTGGCC TTGCTGAATCCTCGCTTTACCTCCATTCTTAGTGGCTTTGGAACTGGAANCAACCTTCTT TGCACTATTCCTTGGCGGGGTTCTGAGAAGGTTCTGTANTGGGAAGGACAGCTGTCCACA ATTTCCTGAGAAGAGCATCAAGGGGGTCTTGGGCATCTGCAAGGTTTCCTTG
Restriction Sites	NotI-NotI
ACCN	AL832349
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832349.1
RefSeq Size	4296 bp
RefSeq ORF	4296 bp
Locus ID	831
Domains	Calpain_inhib
Gene Summary	The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

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