VPS50 (NM_024553) Human Untagged Clone
CAT＃: SC107550
CCDC132 (untagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 2
CNY 2400.00
CNY 6270.00
| Cited in 1 publication. |
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCDC132; VPS54L |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_024553 edited
CAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACC GTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGCTT CCTAGTGTCAGGGCCAGCTGTGTAGTGGCTCGGTGTGATTTGTTAGCTCTTTGAGGCAGG GTACCCTCCTCAGGATTTCGATATGCAAAAAATCAAATCTCTCATGACCCGACAGGGTCT GAAAAGCCCTCAAGAAAGCCTCAGTGATCTTGGTGCCATAGAGAGTCTCCGGGTCCCTGG AAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTGAACAAGAGCT TATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTGTTAAATATGA GCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATAGAGACAAATT GAAACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTGAAAAACAGCC TGCTTATGTAAAGGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTCAATTAGCTGC TGTTATCTGTACAAATGGGAGAAGACACTTGAATATTGCAAAGGAAGGTTTTACTCAAGC TAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGTCAGTTGCTGATTGGACTTCTGAAATC TCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGTTAAGTGAAATGCTGGAGGA GGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCTGCCAGCACTTT TAAACATTACAGTTGTATAAGTGAACTGAATTCAAAGCTGCAAGATACTTTGGAACAGAT TGAGGAACAGCTGGACGTAGCTCTTTCCAAAATCTGCAAGAATTTTGACATTAACCATTA TACCAAGGTTCAACAAGCTTATCGACTTCTTGGAAAAACACAGACAGCAATGGATCAACT TCATATGCACTTCACCCAAGCCATTCACAACACCGTGTTTCAAGTTGTTCTTGGTTATGT GGAACTATGTGCAGGAAACACAGACACAAAATTCCAAAAGCTGCAATATAAGGATCTCTG TACACATGTTACACCAGACAGCTATATTCCATGCCTTGCAGACCTGTGCAAAGCACTATG GGAAGTTATGCTCAGCTATTATAGGACTATGGAATGGCATGAAAAGCATGACAATGAGGA TACTGCTTCAGCTTCTGAAGGGAGTAATATGATAGGTACTGAAGAAACTAATTTTGATCG TGGCTACATAAAAAAGAAATTAGAACATGGACTTACACGAATATGGCAGGATGTTCAGCT AAAAGTAAAAACCTACTTGCTTGGAACTGATTTGTCTATATTCAAATATGATGATTTCAT CTTTGTTTTGGATATAATCAGCAGGTTGATGCAAGTTGGAGAAGAATTTTGTGGTAGCAA GTCTGAAGTTTTACAGGAATCTATTAGAAAACAAAGTGTCAATTATTTCAAGAATTACCA TAGAACACGGCTCGATGAACTGAGAATGTTCTTAGAGAATGAGACTTGGGAACTTTGTCC TGTTAAGTCAAATTTCAGCATCTTGCAACTTCATGAATTTAAATTCATGGAACAGTCTCG CTCCCCATCAGTTTCACCTAGTAAACAGCCAGTCTCAACTTCTTCAAAAACAGTGACCTT GTTTGAGCAGTACTGTAGTGGTGGGAATCCATTTGAAATTCAGGCCAACCACAAAGATGA AGAAACAGAAGATGTCTTAGCTTCTAATGGGTATGAATCTGATGAACAAGAAAAGAGTGC CTATCAAGAGTATGACAGTGACAGTGATGTTCCTGAGGAACTCAAACGAGACTATGTGGA TGAGCAGACAGGAGATGGTCCTGTGAAAAGTGTTTCTCGGGAAACTCTAAAAAGCAGGAA GAAATCAGATTACAGTCTAAATAAAGTGAATGCACCTATCTTAACAAATACAACATTGAA CGTCATAAGACTTGTTGGAAAATATATGCAGATGATGAACATTCTTAAGCCAATTGCCTT TGATGTTATTCATTTCATGTCTCAACTATTTGATTATTACTTGTATGCAATATATACCTT TTTTGGTCGGAATGATTCATTGGAATCAACTGGACTCGGCCTTAGTAGTAGTAGACTAAG AACAACTCTAAACAGAATACAAGAAAGCCTTATTGATCTAGAAGTTTCAGCTGATCCTAC TGCCACACTCACAGCAGCAGAAGAAAGAAAGGAGAAGGTGCCAAGTCCACACCTCAGTCA CCTAGTGGTTTTGACATCTGGGGATACGTTGTATGGGTTGGCAGAAAGAGTGGTAGCCAC GGAATCCTTGGTATTCTTGGCTGAACAGTTTGAGTTCCTTCAGCCACATCTGGATGCTGT GATGCCTGCAGTCAAAAAGCCCTTTCTTCAGCAGTTCTATTCTCAGACAGTCTCAACCGC CAGTGAACTACGGAAACCAATTTACTGGATTGTAGCTGGTAAAGCCCTTGATTATGAACA GATGCTGCTTCTCATGGCTAATGTGAAATGGGATGTAAAAGAAATTATGTCACAGCACAA CATATATGTAGATGCACTATTAAAGGAATTTGAGCAGTTTAACAGGAGGCTAAATGAAGT TTCTAAGAGAGTTCGCATACCCTTGCCTGTGTCTAATATACTTTGGGAACATTGTATACG ATTGGCTAATCGAACTATTGTAGAAGGATATGCCAATGTCAAGAAATGCAGTAATGAGGG TCGTGCCCTGATGCAACTGGATTTTCAACAGTTTTTAATGAAACTTGAAAAACTAACAGA TATTAGACCCATTCCTGATAAAGAATTTGTAGAAACTTATATTAAAGCTTATTACCTAAC TGAGAATGACATGGAACGGTGGATCAAAGAGCACAGGGAATATTCAACGAAGCAGCTGAC CAATCTGGTGAATGTTTGCCTGGGATCCCATATCAATAAGAAAGCAAGACAAAAACTTCT AGCAGCTATAGATGATATAGACAGACCTAAAAGATAATGAACACAGCTCTCTTTCCTCAA TGGCATTGATCCTCACTCAACATATATGACCTGAAAGCCAGTTTTTTTATGCACTTCTGA CAACTATCTGCTAAGAAAACTTTGTGCATGTTTTTTTGACTGGAAAGTGGAAAATATTGA AATGTGTGTGGTGTTCTCATGACTTTTATATGCTGTGGTCTCTTCAACTTTTGGTCTCAT TTGTTGTAATCTGAAATGATGTTGCCGCCTTGTCATAACAATGGTTATGTGACTACAGTT ATACATTTTACAGAAGAATGTACCATAAGTATATAATTAGAAGAACAGTGGCTTAATATA TGTATGGGAAGTTTATGGAAAATGAAGTTGGCACTTTTCTACCCTCTGAGCTTGGTTCTT AATAAGCATAATGTGAGGGTGAATATGTAGTATCTCCTAATTATGAGCACTGCATGAGAA TTAAAAAACACATGTAAGTAAAATAGTTGAAAAATCAGTATGTTCTCTGTTTTTAAAATG TCAAAGTTTATGTCAGGGTTAATTTAGTTATAACAAAGTGATCATAATGGTGAAATTTAA TAAATATACTCTAGTATGAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCG GTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAG >OriGene 5' read for NM_024553 unedited
GAGGTGGCTTCCTAGTGTCAGGGCCAGCTGTGTAGTGGCTCGGTGTGATTTGTTAGCTCT TTGAGGCAGGGTACCCTCCTCAGGATTTCGATATGCAAAAAATCAAATCTCTCATGACCC GACAGGGTCTGAAAAGCCCTCAAGAAAGCCTCAGTGATCTTGGTGCCATAGAGAGTCTCC GGGTCCCTGGAAAGGAAGAATTCAGGGAACTTCGAGAACAGCCAAGTGACCCTCAAGCTG AACAAGAGCTTATTAATAGTATTGAACAAGTATATTTTTCTGTGGATTCATTTGATATTG TTAAATATGAGCTGGAGAAGCTTCCACCTGTTCTCAATTTGCAAGAATTAGAGGCGTATA GAGACAAATTGAAACAACAGCAAGCTGCAGTATCTAAAAAAGTGGCAGATTTAATCCTTG AAAAACAGCCTGCTTATGTAAAGGAACTTGAAAGAGTTACCTCATTGCAGACAGGTCTTC AATTAGCTGCTGTTATCTGTACAAATGGGAGAAGACACTTGAATATTGCAAAGGAAGGTT TTACTCAAGCTAGTTTAGGCCTTCTTGCAAATCAAAGGAAACGTCAGTTGCTGATTGGAC TTCTGAAATCTCTGAGAACTATAAAAACATTGCAAAGAACAGATGTACGGGTTAAGTGAA ATGCTGGAGGAGGAAGATTATCCAGGAGCTATTCAGTTGTGCCTTGAATGTCAAAAAGCT GCCAGCACTTTTAAACATTACAGNTGTATAAGTGAACTGAATTCAAAGCTGCAGANACTT TGGNACAGATTGAGGAACAGCTGGACGTAACTCTTTCCAAATCTGCAGAATTTTGACATT AACCATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_024553 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024553.2, NP_078829.1 |
| RefSeq Size | 1216 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 55610 |
| UniProt ID | Q96JG6 |
| Gene Summary | Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of the gene encoding alkylglycerol monooxygenase defines a third class of tetrahydrobiopterin-dependent enzymes
,Katrin Watschinger, Markus A. Keller, Georg Golderer, Martin Hermann, Manuel Maglione, Bettina Sarg, Herbert H. Lindner, Albin Hermetter, Gabriele Werner-Felmayer, Robert Konrat, Nicolas Hulo, and Ernst R. Werner,
PNAS, Aug 2010; 107: 13672 - 13677
[VPS50]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221086 | CCDC132 (Myc-DDK-tagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 2 |
CNY 3990.00 |
|
| RC221086L3 | Lenti-ORF clone of CCDC132 (Myc-DDK-tagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 2 |
CNY 5890.00 |
|
| RC221086L4 | Lenti-ORF clone of CCDC132 (mGFP-tagged)-Human coiled-coil domain containing 132 (CCDC132), transcript variant 2 |
CNY 5890.00 |
|
| RG221086 | CCDC132 (tGFP-tagged) - Human coiled-coil domain containing 132 (CCDC132), transcript variant 2 |
CNY 4370.00 |
