NSUN3 (NM_022072) Human Untagged Clone
CAT＃: SC108287
NSUN3 (untagged)-Human NOP2/Sun domain family, member 3 (NSUN3)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MST077; MSTP077 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_022072, the custom clone sequence may differ by one or more nucleotides
ATGCTGACCCAGCTGAAAGCAAAATCAGAGGGGAAGCTTGCAAAACAGATTTGCAAAGTTGTGTTGGATC ATTTTGAAAAACAGTATTCCAAAGAACTCGGAGATGCCTGGAATACAGTAAGGGAGATACTAACATCTCC ATCATGCTGGCAATATGCTGTCCTGCTTAACCGATTCAATTATCCTTTTGAACTGGAAAAGGATTTACAT TTGAAGGGCTATCACACACTCTCTCAGGGATCTTTACCCAACTATCCTAAATCAGTGAAGTGTTACCTTA GCAGAACTCCGGGCCGAATCCCTTCAGAAAGACACCAAATTGGAAACCTGAAAAAATATTATCTCCTAAA TGCTGCTTCTCTTCTCCCAGTGTTGGCTCTGGAATTAAGGGATGGGGAGAAGGTTCTGGATCTCTGTGCT GCTCCTGGAGGGAAATCAATAGCTCTGCTGCAGTGTGCTTGTCCAGGTTATCTTCATTGTAATGAATATG ATAGTCTGAGATTGAGGTGGCTAAGGCAGACGTTGGAATCTTTCATCCCACAGCCTTTGATAAATGTAAT TAAAGTGTCTGAATTGGATGGCAGAAAAATGGGAGATGCCCAGCCTGAAATGTTTGACAAGGTGTTAGTG GATGCTCCGTGTTCAAATGATCGAAGCTGGTTGTTTTCTTCTGACTCTCAGAAGGCATCCTGTAGGATAA GTCAAAGGAGGAATTTGCCTCTTCTACAGATAGAGCTGTTAAGGTCTGCAATTAAGGCCTTACGTCCTGG AGGGATACTTGTATACTCTACATGCACGCTTTCCAAGGCAGAAAATCAAGATGTGATCAGTGAAATTTTA AACTCCCACGGTAACATCATGCCTATGGACATTAAAGGAATAGCAAGGACTTGCTCCCACGACTTCACAT TTGCTCCCACTGGCCAGGAATGTGGGCTCTTAGTGATTCCAGATAAGGGCAAAGCCTGGGGCCCAATGTA TGTAGCCAAATTGAAGAAATCATGGAGCACAGGAAAATGGTGA >OriGene 5' read for NM_022072 unedited
TTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACCAGCGAATCCCGGA AGTCAGACTGTTTTTTTCAGTTCCCTGGAGGCTTTTTGATACTGATTCGCGTACACCTGT TGTTTGAAAGCTCTCAGCGGGACAATGCTGACCCAGAGACACGTCCTTGATATGTTTTCC AGTCTGGTCTTGAACTGGGAAATGATCCTCTCGCCTCGTCCCTGCAAAGCATGAGCCAGC TGGAGTACAGTGGCGCGATCTCGGCTCACTGCAACCTCCACCTCCTGAGTTCAAGCGATT CTCCCACCTCAGCCCCCTGAGTAGCTGAAAGCAAAATCAGAGGGGAAGCTTGCAAAACAG ATTTGCAAAGTTGTGTTGGATCATTTTGAAAAACAGTATTCCAAAGAACTCGGAGATGCC TGGAATACAGTAAGGGAGATACTAACATCTCCATCATGCTGGCAATATGCTGTCCTGCTT AACCGATTCAATTATCCTTTTGAACTGGAAAAGGATTTACATTTGAAGGGCTATCACACA CTCTCTCAGGGATCTTTACCCAACTATCCTAAATCAGTGAAGTGTTACCTTAGCAGAACT CCGGGCCGAATCCCTTCAGAAAGACACCAAATTGGAAACCTGAAAAAATATTATCTCCTA AATGCTGCTTCTCTTCTCCCAGTGTTGGCTCTGGAATTAAGGGATGGGGAGAAGGTTCTG GATCTCTGTGCTGCTCCTGGAGGGAAATCAATAGCTCTGCTGCAGTGTGCTTGTCCAGGT TATCTTCATTGTAATGAATATGATAGTCTGAGATTGANGTGGCTAANGCAGACGTTGGAA ATCTTCATCCCACAGCCTTTGATANATGTACTTAAAGTGTCTGAATTGCATGCCAGAAAA TGGGAGATCCCCACCCTGAATGTTGACAAGGTGTAGTGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022072 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022072.2, NP_071355.1 |
| RefSeq Size | 1466 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 63899 |
| UniProt ID | Q9H649 |
| Domains | Nol1_Nop2_Sun |
| Gene Summary | Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met) (PubMed:27497299, PubMed:27214402, PubMed:27356879). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position (PubMed:27497299, PubMed:27214402, PubMed:27356879). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27497299, PubMed:27356879).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204612 | NSUN3 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 3 (NSUN3) |
CNY 3656.00 |
|
| RC204612L1 | Lenti ORF clone of Human NOP2/Sun domain family, member 3 (NSUN3), Myc-DDK-tagged |
CNY 6056.00 |
|
| RC204612L2 | Lenti ORF clone of Human NOP2/Sun domain family, member 3 (NSUN3), mGFP tagged |
CNY 5890.00 |
|
| RC204612L3 | Lenti ORF clone of Human NOP2/Sun domain family, member 3 (NSUN3), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204612L4 | Lenti ORF clone of Human NOP2/Sun domain family, member 3 (NSUN3), mGFP tagged |
CNY 6056.00 |
|
| RG204612 | NSUN3 (tGFP-tagged) - Human NOP2/Sun domain family, member 3 (NSUN3) |
CNY 5256.00 |
|
| SC320959 | NSUN3 (untagged)-Human NOP2/Sun domain family, member 3 (NSUN3) |
CNY 3656.00 |
