MRPS25 (NM_022497) Human Untagged Clone
CAT＃: SC108433
MRPS25 (untagged)-Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein
CNY 2400.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COXPD50; MRP-S25; RPMS25 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108433 sequence for NM_022497 edited (data generated by NextGen Sequencing)
ATGCCCATGAAGGGCCGCTTCCCCATCCGCCGCACCCTGCAATATCTGAGCCAGGGGAAC GTGGTGTTCAAGGACTCCGTGAAGGTCATGACAGTGAATTACAACACGCATGGGGAGCTG GGCGAGGGCGCCAGGAAGTTTGTGTTTTTCAACATACCTCAGATTCAATACAAAAACCCT TGGGTGCAGATCATGATGTTTAAGAACATGACGCCGTCACCCTTCCTGCGATTCTACTTA GATTCTGGGGAGCAGGTCCTGGTGGATGTGGAGACCAAGAGCAATAAGGAGATCATGGAG CACATCAGAAAAATCTTGGGGAAGAATGAGGAAACCCTCAGGGAAGAGGAGGAGGAGAAA AAGCAGCTTTCTCACCCAGCCAACTTCGGCCCTCGAAAGTACTGCCTGCGGGAGTGCATC TGTGAAGTGGAAGGGCAGGTGCCCTGCCCCAGCCTGGTGCCATTACCCAAGGAGATGAGG GGGAAGTACAAAGCCGCTCTGAAAGCCGATGCCCAGGACTAA Clone variation with respect to NM_022497.3 >OriGene 5' read for NM_022497 unedited
TATAGGCGGCCCGCGAATTCGCACGAGGCTGGCTCTGGGGAGAAGCCGCGTGAGATCCGC GCGGGTGCTAGCTAGTCCTTTCTCGTCGCTGCTCGGCTCGCGGCCCGTGGGGTCGGCCCC GCCACCGTTGCCGCCATGCCCATGAAGGGCCGCTTCCCCATCCGCCGCACCCTGCAATAT CTGAGCCAGGGGAACGTGGTGTTCAAGGACTCCGTGAAGGTCATGACAGTGAATTACAAC ACGCATGGGGAGCTGGGCGAGGGCGCCAGGAAGTTTGTGTTTTTCAACATACCTCAGATT CAATACAAAAACCCTTGGGTGCAGATCATGATGTTTAAGAACATGACGCCGTCACCCTTC CTGCGATTCTACTTAGATTCTGGGGAGCAGGTCCTGGTGGATGTGGAGACCAAGAGNCAT AAGGAGATCATGGAGCACATCAGAAAAATCTTGGGGAAGAATGAGGAAACCCTCAGGNAA GAGGANGAGGAGAANAAGCAGCTTTCTCACCCAGCCAACTTCGCCCTCGAAAGTACTGNC TGCGGNAGTGCATCTGTGAGNNGGAAGGGGGCAGTGCCCTGCCCAGCCTGGTGCCCTTAC CCAGGAAATGAGGGGGGAATACAAAACCCGTCTGAAAGCCGATGCCCAGACTAAGCCCAN GTACTGNGGCTGGGGGAAAATGGTTCCTACCTGGGGAAATGGAAAGGGGATTCCTTGGCC CGGAACCCCCCCGGTTTTTGCCTGGAAACTGGGAAAACTTTTGTTTAAAGGCTTTCAACC TGGTAAAGGGTGGGGAAAGAGGGGGGGNAAAACCAACCCCAAAAAAAAACCCCGGCTGGG GAAAAAAACCTTCTATAACCTTTACGGTTTGGGTTTAAAAAAAAGGGGCCTGAAAAAAGA GTTG >OriGene 3' read for NM_022497 unedited
NTTTCTCTGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTGAAAAACTGTTAAAATTTAAGGTTGGNGAATAACATTTGGGAAGTAGTAGAACT TTTGCATATAAAAAATGGCTTTTTCTTTAGGAAAAGTATAATCCAATTTATTTTAATGCA CCAATAGCATTCATGGGACTCAAATGCTGGCTGCCACACACAAACTCAAGGGCTTGGAAT ATTTTNTCTACAGTTCTCTCTGTTCTACGGATTATCTTGTAAACCAGGGTTCAACTAGTT TTATAACTGAAAAGCTGCACATTTTTCACAGTACAAGCCGAAGTTTTTACCTGTAGTTTG GACTTCTCTGTTAAAATGTAACTGCATTACCAGCCCTTTTAAAGGCATCTATCTATCAAA GGAAAATTTGGGGGTTAAATTTTCTTGGGACCGTTTCTGTAACCTTTGCCCTTCACAATA TAAAAAATATTGGTTTTGCCATTACATTTTAATGCCAGGTTTAAAACCTGTTGAAAGCTG CAGCTTTATACAGCTTTCTTCTCCCTGTGAAATCACTTTGTTTATGTGATGGCATTTAAA AAATAGCATGTTCTTTTTAAACTGAATTTTATATCTAATCAATGTCTTCAGTCTTGAAGA AGAATTTGCATTGTTGCGTTTGTATATAGAGTATTGCAGCGCATGAATTACCTTTATGCA TTTTATTAAATGCTGTTTCAATGTGCCATTATTGTTGGGGCCTAATCCACCACCCTAATG AGGTTTATCCTATTAAAGGGGAATTAAAGACTAACATGCCTCCCTGCCCTTAAATTTTTC CACATGAAAAGAAGAAAGGCCCTGGCCCCCAAACCAATGCACGGCTTTTTTTGGGTTATG GAACAAGAAGCATCCTGGGGGGCCCGGCCTTTAACAGTCTTGGCGCCCCACCTTAGCCTT AGCGGCCAGAGGGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022497 |
| Insert Size | 5270 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022497.3, NP_071942.1 |
| RefSeq Size | 4574 bp |
| RefSeq ORF | 522 bp |
| Locus ID | 64432 |
| UniProt ID | P82663 |
| Domains | L51_S25_CI-B8 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201353 | MRPS25 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
|
| RC201353L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC201353L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG201353 | MRPS25 (tGFP-tagged) - Human mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein |
CNY 4000.00 |
