FCHSD2 (NM_014824) Human Untagged Clone
CAT＃: SC108752
FCHSD2 (untagged)-Human FCH and double SH3 domains 2 (FCHSD2)
CNY 7528.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NWK; NWK1; SH3MD3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108752 sequence for NM_014824 edited (data generated by NextGen Sequencing)
ATGCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAACTGAAAAACATTCAAGTTGAG CAGATGACAAAACTTCAAGCCAAACATCAAGCAGAATGTGATTTGCTTGAAGATATGAGG ACATTCAGTCAGAAGAAGGCTGCTATTGAAAGAGAGTATGCACAGGGTATGCAGAAGTTG GCTAGTCAATACCTGAAGAGAGATTGGCCTGGAGTAAAAGCTGATGATCGGAATGATTAC AGGAGCATGTATCCCGTTTGGAAATCTTTTCTCGAGGGAACAATGCAGGTAGCCCAGTCT CGGATGAATATATGTGAAAACTATAAAAACTTCATTTCTGAGCCTGCAAGGACAGTGAGA AGCTTAAAAGAACAGCAACTAAAAAGGTGTGTGGACCAGTTGACAAAGATCCAAACTGAA TTACAAGAGACAGTGAAAGATTTAGCTAAAGGCAAAAAGAAATACTTTGAGACTGAACAG ATGGCTCATGCAGTACGAGAGAAAGCTGACATCGAGGCAAAATCTAAACTTAGTCTTTTT CAATCAAGAATCAGTTTACAGAAGGCAAGTGTAAAGTTAAAAGCCCGGCGATCTGAGTGT AATTCCAAAGCTACCCACGCAAGGAATGATTATCTTCTTACCCTAGCGGCAGCAAATGCA CATCAGGATCGCTACTATCAAACAGATTTAGTTAACATTATGAAGGCTCTTGATGGAAAT GTGTATGATCATCTCAAGGATTATTTAATAGCCTTCAGCCGGACTGAGCTAGAAACATGC CAAGCTGTGCAGAACACATTCCAGTTTTTATTAGAAAACTCCAGCAAGGTGGTCCGGGAC TACAATCTTCAGCTGTTTTTGCAAGAAAACGCTGTATTTCACAAACCCCAGCCCTTCCAG TTCCAGCCTTGTGACAGTGATACTAGCCGACAGTTAGAATCAGAAACTGGGACCACAGAG GAGCACAGTCTAAATAAGGAAGCTCGAAAATGGGCCACACGTGTGGCACGTGAGCATAAA AACATTGTTCACCAACAACGGGTTCTAAATGATCTGGAGTGTCATGGAGCTGCTGTATCA GAACAAAGCCGAGCAGAGCTAGAACAGAAAATAGATGAAGCTAGAGAAAATATTCGTAAA GCAGAGATAATTAAATTGAAAGCTGAAGCCCGGTTGGACCTGCTAAAGCAGATTGGTGTT TCTGTGGACACATGGCTAAAGAGTGCCATGAACCAAGTAATGGAAGAACTGGAAAATGAG CGATGGGCCCGCCCTCCTGCAGTGACCAGTAATGGCACTTTACACTCGCTTAATGCAGAT ACCGAAAGAGAAGAAGGCGAAGAGTTTGAAGATAACATGGATGTTTTCGATGACAGCAGT TCCAGCCCTTCTGGCACCTTAAGAAATTATCCACTCACCTGCAAAGTTGTTTATTCCTAC AAGGCTTCTCAACCAGATGAGTTGACCATTGAGGAACATGAGGTGTTAGAAGTGATTGAA GATGGAGATATGGAAGACTGGGTAAAGGCTCGAAATAAAGTTGGCCAAGTGGGTTATGTG CCAGAAAAGTACCTACAGTTTCCCACCTCGAACAGCCTCCTGAGCATGCTGCAGTCCCTG GCCGCTTTGGACAGTCGGTCACACACGTCCAGCAATTCCACGGAAGCAGAACTCGTTTCA GGCAGCCTCAACGGAGATGCCAGTGTATGTTTTGTGAAAGCACTTTATGATTATGAGGGC CAGACAGATGATGAGTTATCTTTTCCTGAGGGAGCAATAATCCGTATCTTGAACAAAGAA AACCAAGATGATGATGGCTTCTGGGAAGGGGAATTCAATGGGCGTATTGGAGTTTTCCCA TCGGTGCTAGTGGAAGAACTTTCAGCCTCAGAAAATGGTGACACTCCATGGATGAGAGAG ATTCAGATCTCTCCTTCCCCCAAGCCACACGCCTCCCTGCCTCCACTGCCGTTGTACGAC CAGCCTCCCAGCAGCCCCTACCCCAGCCCAGATAAGAGGAGCTCCCTGTACTTTCCCCGG TCTCCTTCAGCAAACGAAAAAAGCCTTCATGCTGAGTCACCAGGATTCTCACAGGCATCA AGGCATACTCCTGAGACCTCATATGGCAAACTGCGACCTGTCCGGGCAGCTCCCCCTCCA CCTACACAGAATCACCGAAGGCCAGCAGAGAAGATTGAAGATGTGGAAATCACACTGGTG TGA Clone variation with respect to NM_014824.2 >OriGene 5' read for NM_014824 unedited
GGCCGCGAATTCGGCACGAGGCTTCCCGGTGCTCCTCCCTCGTCTCCTCACACTCGCTCT CTGGCTGGGGTCCGCCTGCCGCCCGCTGGCCTGCTCCCTCCGGGGCAAGTCTTGCTTCTT AAGCGCTCCTTTCACACACGCGCGCGCTCGCTGGGCCGTCCCCTCCTCTCCGGCCCTCCT CCTCCTCCTGGTCCTTGCTAACGCTGCCGTCGGGGAGGATTGATGTCCCTTCAGCATCAT GCAGCCGCCGCCGAGGAAGGTGAAAGTTACACAAGAACTGAAAAACATTCAAGTTGAGCA GATGACAAAACTTCAAGCCAAACATCAAGCAGAATGTGATTTGCTTGAAGATATGAGGAC ATTCAGTCAGAAGAAGGCTGCTATTGAAAGAGAGTATGCACAGGGTATGCAGAAGTTGGC TAGTCAATACCTGAAGAGAGATTGGCCTGGAGTAAAAGCTGATGATCGGAATGATTACAG GAGCATGTATCCCGTTTGGAAATCTTTTCTCGAGGGAACAATGCAGGTAGCCCAGTCTCG GATGAATATATGTGAAAACTATAAAAACTTCATTTCTGAGCCTGGCAGGACAGTGAGAAG CTTAAAAGAACAGCACTAAAAAGGTGTGTGGACCAGTTGACAAAGATCCCAACTGGGATT ACAGAGACAGTGAAAGATTTAGCTAAAGGCNNAAAGAAATACTNTGAGACTNGACAGATG GCTCATGCAGTACGAGAGAAAGCTGACATCGA >OriGene 3' read for NM_014824 unedited
TTTAGCTTGGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAATTCC ATTTTTTTTATTATTCAACATTTTATACATAATAAATACAAACTTTTTACAGCCACTGTA AAGAAAGCACATCTGCACAGAGGCTCCCTCCGAGCCCTGACCTGTGCACTGTGGTGCTGG ATTCCATATCCTGGATGGAGGGGTTATTTTTTAAAAAGGAGGCATGTTTTCACAACTTTG TTTTTTAAAATAAAATTAGCAGCTCTTCCAAAAAATATTTTAAAATACAACAAAAGATTT CAAATAACCCTTTGAGGTTGTGGAAAACCCAAATTCAAGGACAATTTGGCTAGCTAAAGA CAGAATACAAGACTGGGTGGCAAGAACTGCTCTATTTAATAAGCATTTGAAGATTTTTAA TATGTTAGCAAACTTTAAAATCTTAAAAAAACCACCCTTTTAATTTCTTTTTTAAACTTA AGAATAACTTTGATAAACACAAAAGACCTAAAATAGATCAACAGGTTAAGCAAGTGTAAT ACACAACACCGAATAACAACCCCAACCCATGGCAAATATTCCTTACCGGCGTTTTCGTAC CTGACGTGTTCCACGATTTACAAATTTCTTCACTCCCCACTTTACTCTTCGCCCATACTT CCATGGCGCAATTCCCCGCACCCCCCCCACTTTCTTAATTCCCCTTCCACTTCCGCCCCC CGCTTGTCTCCCCCCTCTCCCCCATTCCCACCCCCCCCCCCCCCGGATTTCCACCACCCA CAATATCTCGCACGGTTTACTTTTTCCCTCACCCTCTCTCACCTACAATTAGCCTACCCA CCCCATCCACCCCTATTAACCCCTTTTACGCCACCCTTTATACACTTAACCATCCCTTTC TCCTTTTTTTCATATACCCCCTACGCTCTTTACACCTCCATTTATCCCCACTATCCACTT CGTATTTTTTACTCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014824 |
| Insert Size | 4310 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014824.1, NP_055639.1 |
| RefSeq Size | 4507 bp |
| RefSeq ORF | 2055 bp |
| Locus ID | 9873 |
| UniProt ID | O94868 |
| Domains | SH3 |
| Gene Summary | Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed:29887380). Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed:30249660). Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed:29887380). Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (PubMed:29887380). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes (PubMed:29887380).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221241 | FCHSD2 (Myc-DDK-tagged)-Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 7512.00 |
|
| RC221241L1 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), Myc-DDK-tagged |
CNY 9600.00 |
|
| RC221241L2 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), mGFP tagged |
CNY 9912.00 |
|
| RC221241L3 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), Myc-DDK-tagged |
CNY 9600.00 |
|
| RC221241L4 | Lenti ORF clone of Human FCH and double SH3 domains 2 (FCHSD2), mGFP tagged |
CNY 9600.00 |
|
| RG221241 | FCHSD2 (tGFP-tagged) - Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 9112.00 |
|
| SC327995 | FCHSD2 (untagged)-Human FCH and double SH3 domains 2 (FCHSD2) |
CNY 14160.00 |
