Myosin light chain kinase (MYLK) (NM_053031) Human Untagged Clone
CAT＃: SC109473
MYLK (untagged)-Human myosin light chain kinase (MYLK), transcript variant 7
CNY 1200.00
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK1; smMLCK |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC109473 sequence for NM_053031 edited (data generated by NextGen Sequencing)
ATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCCCG CTCAATGCAGAAAAACTAGAATCTGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCT GAGGAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTG GAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTC TGGTTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAG GACGGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGTACACC TGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACG ATGGAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGA Clone variation with respect to NM_053031.2 >OriGene 5' read for NM_053031 unedited
GGGTCACCAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGCAGAACCTC GGCTCTCAGGAGGTCCTTGTTCCAGGGAACAGCTGCTTCTCTGGGGCTGGGCTCTACTCC CTGCAGCCCCTCGCACTACCCAGCTGGAACCAGGGACAACGCCTGAGTCCAACCCTCGTG TCTATTTTCCAGAAAACGGGCAATGCTGTGAGAGCCATTGGAAGACTGTCCTCTATGGCA ATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGCCCGCTCAAT GCAGAAAAACTAGAATCTGAAGAAGATGTGTCCCAAGCTTTCCTTGAGGCTGTTGCTGAG GAAAAGCCTCATGTAAAACCCTATTTCTCTAAGACCATTCGCGATTTAGAAGTTGTGGAG GGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCAGACCCCGAGGTTGTCTGG TTCAAAGATGACCAGTCAATCAGGGAGTCCCGCCACTTCCAGATAGACTACGATGAGGAC GGGAACTGCTCTTTAATTATTAGTGATGTTTGCGGGGATGACGATGCCAAGTACACCTGC AAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATTGTGGAAACGATG GAGGAAGGTGAAGGGGAAGGGGAAGAGGAAGAAGAGTGAAACAAAGCCAGAGAAAAGCAG TTTCTAAGTCATATTAAAAGGACTATNTCTCTAAAACTCAAAANAAAAAAAAAAACTCGA CTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGGCATCCCTG TGACCCCTNNCCCAGTGCCTCTCCTGGCCCCTGGAAGNNTGCACTNNCAGTNCCCACCCA GCCCTGTCCTAATAAAANTAAAGTGCATCANTTTGTCTGACTAGGTGTCT >OriGene 3' read for NM_053031 unedited
CGTNACAACTATGNAACCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTGAGT TTTAGAGAAAAGTCCTTTTAATATGACTTAGAAACTGCTTTTCTCTGGCTTTGTTTCACT CTTCTTCCTCTTCCCCTTCCCCTTCACCTTCCTCCATCGTTTCCACAATGAGCTCTGCTG TGCAGGTGGCTTCTCCAAGACTGTTGACAGCCTTGCAGGTGTACTTGGCATCGTCATCCC CGCAAACATCACTAATAATTAAAGAGCAGTTCCCGTCCTCATCGTAGTCTATCTGGAAGT GGCGGGACTCCCTGATTGACTGGTCATCTTTGAACCAGACAACCTCGGGGTCTGGGTATC CTTCAATCTTGCAGTCAAATCTAGCAGCACTTCCCTCCACAACTTCTAAATCGCGAATGG TCTTAGAGAAATAGGGTTTTACATGAGGCTTTTCCTCAGCAACAGCCTCAAGGAAAGCTT GGGACACATCTTCTTCAGATTCTAGTTTTTCTGCATTGAGCGGGCTGGTTGGTGACCCTG TTGAGGATTTCCTGCCACTGAGCCCTGAGATCATTGCCATAGAGGACAGTCTTCCAATGG CTCTCACAGCATTGCCCGTTTTCTGGAAAATAGACACGAGGGTTGGACTCAGGCGTTGTC CCTGGTTCCAGCTGGGTAGTGCGAGGGGCTGCANGGAGTAGAGCCCAGCCCCAGAGAAGC AGCTGTTCCCTGGAACAAGGACCTCCTGAGAGCCGAGGTTCTGCTCGTGCCGAATTCGCG GCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTA TATAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCACGGGGCGGGGTTATTACG ACATTTTGGAAAGTCCCGTTGATTTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_053031 |
| Insert Size | 800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053031.2, NP_444259.1 |
| RefSeq Size | 2676 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 4638 |
| UniProt ID | Q15746 |
| Domains | ig, IGc2, IG |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction |
| Gene Summary | This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (7) encodes the shorter isoform of kinase related protein, telokin. The first exon corresponds to intron 30 and the remainder of the transcript corresponds to the last two exons of the gene. It is shorter than variant 8 by one codon at the splicing junction between the first two exons. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218513 | MYLK (Myc-DDK-tagged)-Human myosin light chain kinase (MYLK), transcript variant 7 |
CNY 1200.00 |
|
| RC218513L1 | Lenti ORF clone of Human myosin light chain kinase (MYLK), transcript variant 7, Myc-DDK-tagged |
CNY 3600.00 |
|
| RC218513L2 | Lenti ORF clone of Human myosin light chain kinase (MYLK), transcript variant 7, mGFP tagged |
CNY 5890.00 |
|
| RC218513L3 | Lenti ORF clone of Human myosin light chain kinase (MYLK), transcript variant 7, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC218513L4 | Lenti ORF clone of Human myosin light chain kinase (MYLK), transcript variant 7, mGFP tagged |
CNY 5890.00 |
|
| RG218513 | MYLK (tGFP-tagged) - Human myosin light chain kinase (MYLK), transcript variant 7 |
CNY 2800.00 |
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